Information on EC 2.5.1.84 - all-trans-nonaprenyl-diphosphate synthase [geranyl-diphosphate specific]

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.5.1.84
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RECOMMENDED NAME
GeneOntology No.
all-trans-nonaprenyl-diphosphate synthase [geranyl-diphosphate specific]
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geranyl diphosphate + 7 isopentenyl diphosphate = 7 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
nonaprenyl diphosphate biosynthesis I
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isoprenoid biosynthesis
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Terpenoid backbone biosynthesis
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Biosynthesis of secondary metabolites
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SYSTEMATIC NAME
IUBMB Comments
geranyl-diphosphate:isopentenyl-diphosphate transtransferase (adding 7 isopentenyl units)
(2E,6E)-Farnesyl diphosphate and geranylgeranyl diphosphate are less effective as substrates than geranyl diphosphate. The enzyme is involved in the synthesis of the side chain of menaquinone-9 [1]. In Oryza sativa the enzyme SPS2 is involved in providing solanesyl diphosphate for plastoquinone-9 formation [3].
CAS REGISTRY NUMBER
COMMENTARY hide
83745-07-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
isoform SPS2 weakly complements the yeast coq1 disruptant, which does not form ubiquinone. Transformants produce ubiquinone 9 instead of the native yeast product ubiquinone 6. SPS2 is involved in providing solanesyl diphosphate for plastochinone-9 formation
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + 6 isopentenyl diphosphate
6 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram
44% of the rate with geranyl diphosphate
products range from C 15 to C45
-
?
all-trans-farnesyl diphosphate + 6 isopentenyl diphosphate
6 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram
dimethylallyl diphosphate + 8 isopentenyl diphosphate
8 diphosphate + nonaprenyl diphosphate
show the reaction diagram
18.1% of the activity with geranyl diphosphate, OsSPS2
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-
?
dimethylallyl diphosphate + isopentenyl diphosphate
diphosphate + ?
show the reaction diagram
18% of the rate with geranyl diphosphate
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-
?
farnesyl diphosphate + 6 isopentenyl diphosphate
6 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram
farnesyl diphosphate + 6 isopentenyl diphosphate
6 diphosphate + nonaprenyl diphosphate
show the reaction diagram
farnesyl imidodiphosphate + isopentenyl diphosphate
?
show the reaction diagram
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-
-
-
?
farnesyl methylenediphosphonate + isopentenyl diphosphate
?
show the reaction diagram
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-
-
-
?
farnesyl phosphosulfate + isopentenyl diphosphate
?
show the reaction diagram
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-
-
-
?
geranyl diphosphate + 7 isopentenyl diphosphate
7 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram
geranyl diphosphate + 7 isopentenyl diphosphate
7 diphosphate + nonaprenyl diphosphate
show the reaction diagram
geranyl diphosphate is the preferred substrate, OsSPS2
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-
?
geranyl diphosphate + isopentenyl diphosphate
?
show the reaction diagram
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-
-
-
?
geranyl imidodiphosphate + isopentenyl diphosphate
?
show the reaction diagram
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-
-
-
?
geranyl methylenediphosphonate + isopentenyl diphosphate
?
show the reaction diagram
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-
-
?
geranyl phosphosulfate + isopentenyl diphosphate
?
show the reaction diagram
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-
-
-
?
geranylgeranyl diphosphate + 5 isopentenyl diphosphate
5 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram
geranylgeranyl diphosphate + 5 isopentenyl diphosphate
5 diphosphate + nonaprenyl diphosphate
show the reaction diagram
34.6% of the activity with geranyl diphosphate, OsSPS2
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
farnesyl diphosphate + 6 isopentenyl diphosphate
6 diphosphate + nonaprenyl diphosphate
show the reaction diagram
Q75HZ9
OsSP2 is involved in the supply of all-trans-nonaprenyl diphosphate for plastoquinone-9 biosynthesis
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?
geranyl diphosphate + 7 isopentenyl diphosphate
7 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram
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the enzyme is involved in the synthesis of the side chain of menaquinone-9
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
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with MgCl2 nonaprenyl diphosphate is the longest product, with MnCl2 or CoCl2, the longest products are C50 and C55, respectively, partially purified enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-O-octyl-beta-D-glucopyranoside
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0.1%, 44% inhibition
Ca2+
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1 mM (in absence of Mn2+ or Mg2+), complete inhibition
deoxycholate
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0.01%, 70% inhibition
farnesyl methylenediphosphate
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above 0.005 mM
Farnesyl phosphosulfate
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above 0.1 mM
Imidodiphosphate
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Methylenediphosphonate
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SDS
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0.005%, 78% inhibition
Triton X-100
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0.05%, 53% inhibition
Zn2+
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1 mM (in absence of Mn2+ or Mg2+), complete inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bacitracin
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0.03-0.06%, 2fold stimulation
bovine serum albumin
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stimulates
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Digitonin
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; 0.005%, 29fold activation
taurodeoxycholate
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0.005%, 1.3fold activation
Tween 80
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0.05-0.1%, 2fold stimulation
Tween-80
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0.005%, 1.4fold activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00025 - 0.00076
all-trans-farnesyl diphosphate
0.001
all-trans-farnesyl methylenediphosphonate
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pH 7.4, 37C
0.0052
farnesyl diphosphate
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pH 7.4, 37C
0.0057
Farnesyl imidodiphosphate
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pH 7.4, 37C
0.0133
Farnesyl phosphosulfate
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pH 7.4, 37C
0.00075 - 0.0043
geranyl diphosphate
0.0077
Geranyl imidodiphosphate
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pH 7.4, 37C
0.0054
Geranyl methylenediphosphonate
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pH 7.4, 37C
0.0909
Geranyl phosphosulfate
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pH 7.4, 37C
0.017 - 0.023
isopentenyl diphosphate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000787
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8
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in Tris-HCl buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9.5
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pH 7.0: about 60% of maximal activity, pH 9.5: about 60% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
high expression; high expression level
Manually annotated by BRENDA team
high expression; high expression level
Manually annotated by BRENDA team
low expression
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
; isoform SPS2 shows a putative transit peptide of 57 amino acids for plastidal localization at the N-terminus
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
74000 - 78000
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gel filtration
78000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 34000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
isoform SPS2 shows a putative transit peptide of 57 amino acids for plastidal localization at the N-terminus
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, stable for at least 2 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in yeast
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