Information on EC 2.5.1.86 - trans,polycis-decaprenyl diphosphate synthase

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The expected taxonomic range for this enzyme is: Mycobacterium

EC NUMBER
COMMENTARY hide
2.5.1.86
-
RECOMMENDED NAME
GeneOntology No.
trans,polycis-decaprenyl diphosphate synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate = 7 diphosphate + trans,octacis-decaprenyl diphosphate
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
mono-trans, poly-cis decaprenyl phosphate biosynthesis
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isoprenoid biosynthesis
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Terpenoid backbone biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(2Z,6E)-farnesyl-diphosphate:isopentenyl-diphosphate farnesylcistransferase (adding 7 isopentenyl units)
The enzyme is involved in the biosynthesis of decaprenyl phosphate, which plays a central role in the biosynthesis of essential mycobacterial cell wall components, such as the mycolyl-arabinogalactan-peptidoglycan complex and lipoarabinomannan [2].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate
diphosphate + decaprenyl diphosphate + nonaprenyl diphosphate + octaprenyl diphosphate
show the reaction diagram
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate
7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate
show the reaction diagram
geranyl diphosphate + isopentenyl diphosphate
diphosphate + decaprenyl diphosphate + nonaprenyl diphosphate + octaprenyl diphosphate
show the reaction diagram
geranylgeranyl diphosphate + isopentenyl diphosphate
diphosphate + decaprenyl diphosphate + octaprenyl diphosphate + nonaprenyl diphosphate
show the reaction diagram
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the major product synthesized is octaprenyl diphosphate, followed by nonaprenyl diphosphate, decaprenyl diphosphate, and heptaprenyl diphosphate
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?
neryl diphosphate + isopentenyl diphosphate
diphosphate + decaprenyl diphosphate + nonaprenyl diphosphate + octaprenyl diphosphate
show the reaction diagram
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decaprenyl diphosphate, nonaprenyl diphosphate, and octaprenyl diphosphate are synthesized. The relative amounts of both decaprenyl diphosphate and octaprenyl diphosphate are greater than that of nonaprenyl diphosphate
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate
7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
absolute requirement for divalent cations. Enzyme activity is optimal in the presence of 1 mM Mg2+. MnCl2 at 0.1 mM also supports the activity. CaCl2 and ZnCl2 are much less effective
Mg2+
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absolute requirement for divalent cations. Enzyme activity is optimal in the presence of 1 mM Mg2+. MnCl2 at 0.1 mM also supports the activity. CaCl2 and ZnCl2 are much less effective
Mn2+
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absolute requirement for divalent cations. Enzyme activity is optimal in the presence of 1 mM Mg2+. MnCl2 at 0.1 mM also supports the activity. CaCl2 and ZnCl2 are much less effective
Zn2+
-
absolute requirement for divalent cations. Enzyme activity is optimal in the presence of 1 mM Mg2+. MnCl2 at 0.1 mM also supports the activity. CaCl2 and ZnCl2 are much less effective
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
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stimulates activity
Triton X-100
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detergent stimulates activity, maximally active in presence of 0.1% Triton X-100
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.084
(2E,6E)-farnesyl diphosphate
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pH 7.9, 37C
0.29
(2Z,6E)-farnesyl diphosphate
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pH 7.9, 37C
0.49
geranyl diphosphate
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pH 7.9, 37C
0.04
geranylgeranyl diphosphate
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pH 7.9, 37C
0.089
isopentenyl diphosphate
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pH 7.9, 37C
0.029
neryl diphosphate
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pH 7.9, 37C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.07
(2E,6E)-farnesyl diphosphate
Mycobacterium tuberculosis
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pH 7.9, 37C
11.5
(2Z,6E)-farnesyl diphosphate
Mycobacterium tuberculosis
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pH 7.9, 37C
0.06
geranyl diphosphate
Mycobacterium tuberculosis
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pH 7.9, 37C
0.03
geranylgeranyl diphosphate
Mycobacterium tuberculosis
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pH 7.9, 37C
0.08
isopentenyl diphosphate
Mycobacterium tuberculosis
-
pH 7.9, 37C
0.16
neryl diphosphate
Mycobacterium tuberculosis
-
pH 7.9, 37C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
39.66
(2Z,6E)-farnesyl diphosphate
Mycobacterium tuberculosis
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pH 7.9, 37C
1810
0.122
geranyl diphosphate
Mycobacterium tuberculosis
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pH 7.9, 37C
175
0.75
geranylgeranyl diphosphate
Mycobacterium tuberculosis
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pH 7.9, 37C
231
0.899
isopentenyl diphosphate
Mycobacterium tuberculosis
-
pH 7.9, 37C
113
5.5
neryl diphosphate
Mycobacterium tuberculosis
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pH 7.9, 37C
1451
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystallization using sparse matrix screens and vapor diffusion, structures of Rv2361c in the apo form, with isopentyl diphosphate bound and with a substrate analogue, citronellyl diphosphate
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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