Information on EC 2.6.1.35 - glycine-oxaloacetate transaminase

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The expected taxonomic range for this enzyme is: Alphaproteobacteria

EC NUMBER
COMMENTARY hide
2.6.1.35
-
RECOMMENDED NAME
GeneOntology No.
glycine-oxaloacetate transaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
glycine + oxaloacetate = glyoxylate + L-aspartate
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
glycine:oxaloacetate aminotransferase
A pyridoxal-phosphate protein.
CAS REGISTRY NUMBER
COMMENTARY hide
37277-90-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
inducible
-
-
Manually annotated by BRENDA team
inducible; strain E1F1
-
-
Manually annotated by BRENDA team
strain E1F1
-
-
Manually annotated by BRENDA team
inducible; strain 7050, DSM 137
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-
Manually annotated by BRENDA team
strain 7050, DSM 137
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glyoxylate + L-aspartate
glycine + oxaloacetate
show the reaction diagram
glyoxylate + L-serine
hydroxypyruvate + glycine
show the reaction diagram
-
transamination at about 30% the rate of L-aspartate
-
r
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glyoxylate + L-aspartate
glycine + oxaloacetate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
requirement
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no metal ion requirement
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
erythro-L-hydroxyaspartate
-
competitive versus L-aspartate and L-serine
L-serine
-
competitive versus L-aspartate
additional information
-
no inhibition by methionine sulfoximine
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
L-asparagine
-
enzyme induction when used as sole nitrogen source during growth
L-glutamine
-
enzyme induction when used as sole nitrogen source during growth
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.43
glyoxylate
-
pH 7.1, 25°C, with substrate L-aspartate
1.9
L-aspartate
-
pH 7.1, 25°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.043
-
partially purified enzyme
0.23
-
activity depends on growth substrates
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
quite stable at 2°C
639966
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2
-
quite stable at pH 7.0
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
2°C, partially purified enzyme, pH 7.0, quite stable
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial, 40fold
-