Information on EC 2.6.1.64 - glutamine-phenylpyruvate transaminase

Word Map on EC 2.6.1.64
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.6.1.64
-
RECOMMENDED NAME
GeneOntology No.
glutamine-phenylpyruvate transaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-glutamine + phenylpyruvate = 2-oxoglutaramate + L-phenylalanine
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
L-glutamine:phenylpyruvate aminotransferase
A pyridoxal-phosphate protein. L-Methionine, L-histidine and L-tyrosine can act as donors. The enzyme has little activity on pyruvate and glyoxylate (cf. EC 2.6.1.15 glutamine---pyruvate transaminase).
CAS REGISTRY NUMBER
COMMENTARY hide
68518-06-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bovine
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
broiler chick, Hubbard strain
-
-
Manually annotated by BRENDA team
mouse
-
-
Manually annotated by BRENDA team
Sprague-Dawley, Buffalo strain
-
-
Manually annotated by BRENDA team
Donryu strain
-
-
Manually annotated by BRENDA team
Sprague-Dawley
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(-)allocystathionine + phenylpyruvate
? + L-phenylalanine
show the reaction diagram
-
-
-
-
r
5-S-L-cysteinyl-L-DOPA + 4-(methylsulfanyl)-2-oxobutanoate
?
show the reaction diagram
-
-
-
-
?
5-S-L-cysteinyldopamine + 4-(methylsulfanyl)-2-oxobutanoate
?
show the reaction diagram
-
transamination of 5 mM 5-S-Lcysteinyldopamine with 1 mM 4-(methylsulfanyl)-2-oxobutanoateat pH 7.4 is about 0.07% the rate at which the enzyme catalyzes transamination between 20 mM L-phenylalanine and 5 mM alpha-oxo-gamma-methiolbutyrate
-
-
?
albizziin + glyoxylate
3-carbamido-2-oxopropanoate + glycine
show the reaction diagram
-
-
-
-
r
CH3Hg-S-Cys + phenylpyruvate
? + L-phenylalanine
show the reaction diagram
-
-
-
-
?
Cys-S-Hg-S-Cys + phenylpyruvate
? + L-phenylalanine
show the reaction diagram
-
-
-
-
?
DL-2,6-diaminoheptanedioate + phenylpyruvate
2-amino-6-oxoheptanedioate + L-phenylalanine
show the reaction diagram
-
-
-
r
DL-homoserine + glyoxylate
4-hydroxy-2-oxobutanoate + glycine
show the reaction diagram
-
-
-
-
r
DL-homoserine + phenylpyruvate
4-hydroxy-2-oxobutanoate + phenylalanine
show the reaction diagram
DL-methionine-SR-sulfoximine + phenylpyruvate
4-methylsulfanyl-2-oxobutanoate-SR-sulfoximine + L-phenylalanine
show the reaction diagram
-
-
-
-
r
DL-penicillamine + phenylpyruvate
3-methyl-3-mercapto-2-oxo-butanoate + L-phenylalanine
show the reaction diagram
-
-
-
r
DL-threonine + phenylpyruvate
3-hydroxy-2-oxobutanoate + L-phenylalanine
show the reaction diagram
-
-
-
-
r
L-3-(1-naphthyl)alanine + 4-(methylsulfanyl)-2-oxobutanoate
?
show the reaction diagram
-
about 0.2% the rate exhibited with L-phenylalanine and alpha-oxo-gamma-methiolbutyrate
-
-
?
L-3-(2-naphthyl)alanine + 4-(methylsulfanyl)-2-oxobutanoate
?
show the reaction diagram
-
about 0.5% the rate exhibited with L-phenylalanine and alpha-oxo-gamma-methiolbutyrate
-
-
?
L-5-N-hydroxyglutamine + phenylpyruvate
4-(N-hydroxycarbamoyl)-2-oxobutanoate + L-phenylalanine
show the reaction diagram
-
-
-
-
r
L-5-N-methylglutamine + glyoxylate
4-(N-methylcarbamoyl)-2-oxobutanoate + glycine
show the reaction diagram
-
-
-
-
r
L-5-N-methylglutamine + phenylpyruvate
4-(N-methylcarbamoyl)-2-oxobutanoate + L-phenylalanine
show the reaction diagram
-
-
-
-
r
L-arginine + pyruvate
5-guanidino-2-oxopentanoate + L-alanine
show the reaction diagram
-
-
-
-
r
L-asparagine + phenylpyruvate
2-oxosuccinamate + L-phenylalanine
show the reaction diagram
L-aspartate + pyruvate
oxaloacetate + L-alanine
show the reaction diagram
-
-
-
-
r
L-cyano-L-alpha-aminobutyrate + glyoxylate
4-cyano-2-oxobutanoate + glycine
show the reaction diagram
-
-
-
-
r
L-cyano-L-alpha-aminobutyrate + phenylpyruvate
4-cyano-2-oxobutanoate + L-phenylalanine
show the reaction diagram
-
-
-
-
r
L-cystathionine + phenylpyruvate
S-(2-oxo-2-carboxyethyl)homocysteine + L-phenylalanine
show the reaction diagram
L-cysteine + glyoxylate
3-mercapto-2-oxopropanoate + glycine
show the reaction diagram
-
-
-
-
r
L-cysteine + phenylpyruvate
3-mercapto-2-oxopropanoate + L-phenylalanine
show the reaction diagram
L-cystine + phenylpyruvate
cystine ketimine + phenylalanine
show the reaction diagram
-
-
-
r
L-DOPA + 4-(methylsulfanyl)-2-oxobutanoate
?
show the reaction diagram
-
about 0.02% the rate exhibited with L-phenylalanine and alpha-oxo-gamma-methiolbutyrate
-
-
?
L-ethionine + glyoxylate
4-ethylsulfanyl-2-oxobutanoate + glycine
show the reaction diagram
-
-
-
-
r
L-ethionine + phenylpyruvate
4-ethylsulfanyl-2-oxobutanoate + L-phenylalanine
show the reaction diagram
-
-
-
-
r
L-gamma-glutamylhydrazone of glyoxylate + glyoxylate
?
show the reaction diagram
-
-
-
-
r
L-gamma-glutamylhydrazone of glyoxylate + phenylpyruvate
?
show the reaction diagram
-
-
-
-
r
L-gamma-glutamylhydrazone of phenylpyruvate + glyoxylate
?
show the reaction diagram
-
-
-
-
r
L-gamma-glutamylhydrazone of phenylpyruvate + phenylpyruvate
?
show the reaction diagram
-
-
-
-
r
L-glutamate + phenylpyruvate
2-oxoglutarate + L-phenylalanine
show the reaction diagram
-
-
-
-
r
L-glutamate-5-ethylester + phenylpyruvate
2-oxoglutarate-5-ethylester + L-phenylalanine
show the reaction diagram
-
-
-
-
r
L-glutamate-5-methylester + phenylpyruvate
2-oxoglutarate-5-methylester + L-phenylalanine
show the reaction diagram
-
-
-
-
r
L-glutamic acid-gamma-methyl ester + glyoxylate
2-oxo-pentandioate-5-methyl ester + glycine
show the reaction diagram
-
liver enzyme L
-
-
r
L-glutamic acid-gamma-methyl ester + phenylpyruvate
2-oxo-pentandioate-5-methyl ester + L-phenylalanine
show the reaction diagram
-
liver enzyme L
-
-
r
L-glutamine + 2-oxo-4-methiolbutyrate
2-oxoglutaramate + L-methionine
show the reaction diagram
-
-
-
-
r
L-glutamine + 2-oxo-4-methyl valerate
2-oxoglutaramate + L-4-methyl-norvaline
show the reaction diagram
-
-
-
-
r
L-glutamine + 2-oxobutyrate
2-oxoglutaramate + 2-aminobutanoate
show the reaction diagram
L-glutamine + 2-oxoglutaramate
2-oxoglutaramate + L-glutamine
show the reaction diagram
-
-
-
-
r
L-glutamine + 2-oxoglutarate
2-oxoglutaramate + L-glutamate
show the reaction diagram
-
-
-
-
r
L-glutamine + 2-oxoisovalerate
2-oxoglutaramate + L-valine
show the reaction diagram
-
-
-
-
r
L-glutamine + 2-oxovalerate
2-oxoglutaramate + L-norvaline
show the reaction diagram
-
-
-
-
r
L-glutamine + 3-bromopyruvate
2-oxoglutaramate + 2-amino-3-bromopropionic acid
show the reaction diagram
-
-
-
-
r
L-glutamine + 3-hydroxypyruvate
2-oxoglutaramate + L-serine
show the reaction diagram
-
-
-
-
r
L-glutamine + 3-indolepyruvate
2-oxoglutaramate + L-tryptophan
show the reaction diagram
-
-
-
-
r
L-glutamine + 3-methyl-2-oxo-pentanoate
2-oxoglutaramate + L-3-methyl-norvaline
show the reaction diagram
-
-
-
-
r
L-glutamine + 4-methylsulfanyl-2-oxobutanoate
2-oxoglutaramate + L-methionine
show the reaction diagram
L-glutamine + alpha-keto-delta-carbamidovalerate
2-oxoglutaramate + L-arginine
show the reaction diagram
-
-
-
-
r
L-glutamine + alpha-keto-n-caproate
2-oxoglutaramate + L-norleucine
show the reaction diagram
-
-
-
-
r
L-glutamine + alpha-ketoisocaproate
2-oxoglutaramate + L-leucine
show the reaction diagram
-
-
-
-
r
L-glutamine + alpha-ketoisovalerate
2-oxoglutaramate + L-valine
show the reaction diagram
-
-
-
-
r
L-glutamine + glyoxylate
2-oxoglutaramate + glycine
show the reaction diagram
L-glutamine + oxaloacetate
2-oxoglutaramate + L-aspartate
show the reaction diagram
-
-
-
-
r
L-glutamine + p-hydroxyphenylpyruvate
2-oxoglutaramate + L-tyrosine
show the reaction diagram
-
-
-
-
r
L-glutamine + phenylpyruvate
2-oxoglutaramate + L-phenylalanine
show the reaction diagram
L-glutamine + pyruvate
2-oxoglutaramate + L-alanine
show the reaction diagram
L-glutamylmethylamide + glyoxylate
2-oxoglutarate-5-methylamide + glycine
show the reaction diagram
-
liver enzyme L
-
-
r
L-glutamylmethylamide + phenylpyruvate
2-oxoglutarate-5-methylamide + L-phenylalanine
show the reaction diagram
-
-
-
-
r
L-histidine + glyoxylate
3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
show the reaction diagram
-
-
-
-
r
L-histidine + phenylpyruvate
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-phenylalanine
show the reaction diagram
-
-
-
-
r
L-histidine + pyruvate
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
show the reaction diagram
L-homocysteine + phenylpyruvate
4-mercapto-2-oxobutanoate + L-phenylalanine
show the reaction diagram
-
-
-
r
L-homocystine + phenylpyruvate
? + L-phenylalanine
show the reaction diagram
-
-
-
-
r
L-kynurenine + 4-(methylsulfanyl)-2-oxobutanoate
?
show the reaction diagram
-
about 23.6% the rate exhibited with L-phenylalanine and alpha-oxo-gamma-methiolbutyrate
-
-
?
L-kynurenine + pyruvate
4-(2-aminophenyl)-2,4-dioxobutanoate + L-alanine
show the reaction diagram
-
-
-
-
r
L-lanthionine + phenylpyruvate
2H-1,4-thiazine-5,6-dihydro-3,5-dicarboxylic acid + L-phenylalanine
show the reaction diagram
-
-
-
r
L-leucine + phenylpyruvate
4-methyl-2-oxopentanoate + L-phenylalanine
show the reaction diagram
L-methionine + 2-oxoglutaramate
L-glutamine + 4-methylsulfanyl-2-oxobutanoate
show the reaction diagram
-
isozymes L and K highly active
-
-
r
L-methionine + glyoxylate
4-methylsulfanyl-2-oxobutanoate + glycine
show the reaction diagram
-
-
-
-
r
L-methionine + phenylpyruvate
4-methylsulfanyl-2-oxobutanoate + L-phenylalanine
show the reaction diagram
L-methionine sulfone + glyoxylate
4-methylsulfonyl-2-oxobutanoate + glycine
show the reaction diagram
-
-
-
-
r
L-methionine sulfone + phenylpyruvate
4-methylsulfonyl-2-oxobutanoate + L-phenylalanine
show the reaction diagram
-
-
-
-
r
L-methionine-SR-sulfoxide + glyoxylate
4-methylsulfinyl-2-oxobutanoate + glycine
show the reaction diagram
-
-
-
-
r
L-methionine-SR-sulfoxide + phenylpyruvate
4-methylsulfinyl-2-oxobutanoate + L-phenylalanine
show the reaction diagram
L-methionine-SR-sulfoximine + glyoxylate
4-methylsulfanyl-2-oxobutanoate-SR-sulfoximine + glycine
show the reaction diagram
-
-
-
-
r
L-methionine-SR-sulfoximine + phenylpyruvate
4-methylsulfanyl-2-oxobutanoate-SR-sulfoximine + L-phenylalanine
show the reaction diagram
-
-
-
-
r
L-norleucine + phenylpyruvate
2-oxohexanoate + L-phenylalanine
show the reaction diagram
-
-
-
r
L-phenylalanine + 2-oxoglutaramate
phenylpyruvate + L-glutamine
show the reaction diagram
-
-
-
-
r
L-phenylalanine + 4-(methylsulfanyl)-2-oxobutanoate
?
show the reaction diagram
-
-
-
-
?
L-phenylalanine + 4-(methylsulfanyl)-2-oxobutanoate
phenylpyruvate + L-methionine
show the reaction diagram
L-phenylalanine + 4-hydroxyphenylpyruvate
phenylpyruvate + L-tyrosine
show the reaction diagram
-
-
-
-
r
L-phenylalanine + 4-methylsulfanyl-2-oxobutanoate
phenylpyruvate + L-methionine
show the reaction diagram
L-phenylalanine + alpha-keto-n-caproate
phenylpyruvate + L-norleucine
show the reaction diagram
-
-
-
-
r
L-phenylalanine + alpha-keto-n-valerate
phenylpyruvate + L-norvaline
show the reaction diagram
-
-
-
-
r
L-phenylalanine + alpha-ketobutyrate
phenylpyruvate + aminobutyrate
show the reaction diagram
-
-
-
-
r
L-phenylalanine + glyoxylate
phenylpyruvate + glycine
show the reaction diagram
-
-
-
-
r
L-phenylalanine + indolepyruvate
phenylpyruvate + L-tryptophan
show the reaction diagram
-
-
-
-
r
L-phenylalanine + pyruvate
phenylpyruvate + L-alanine
show the reaction diagram
L-serine + pyruvate
3-hydroxy-2-oxopropanoate + L-alanine
show the reaction diagram
-
-
-
-
r
L-threonine + pyruvate
3-hydroxy-2-oxobutanoate + L-alanine
show the reaction diagram
-
-
-
-
r
L-tryptophan + pyruvate
indolepyruvate + L-alanine
show the reaction diagram
-
-
-
-
r
L-tyrosine + 2-oxoglutaramate
3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamine
show the reaction diagram
L-tyrosine + 4-(methylsulfanyl)-2-oxobutanoate
?
show the reaction diagram
-
about 9% the rate exhibited with L-phenylalanine and alpha-oxo-gamma-methiolbutyrate
-
-
?
L-tyrosine + glyoxylate
3(4-hydroxyphenyl)-2-oxopropanoate + glycine
show the reaction diagram
-
-
-
-
r
O-carbamoyl-L-serine + glyoxylate
O-carbamoyl-(3-hydroxy-2-oxo-propanoate) + glycine
show the reaction diagram
-
-
-
-
r
phenylpyruvate + L-alanine
L-phenylalanine + pyruvate
show the reaction diagram
-
-
-
-
r
phenylpyruvate + L-glutamine
?
show the reaction diagram
-
about 64% the rate exhibited with L-phenylalanine and alpha-oxo-gamma-methiolbutyrate
-
-
?
phenylpyruvate + L-leucine
?
show the reaction diagram
-
about 25% the rate exhibited with L-phenylalanine and alpha-oxo-gamma-methiolbutyrate
-
-
?
phenylpyruvate + L-methionine
?
show the reaction diagram
-
about 30% the rate exhibited with L-phenylalanine and alpha-oxo-gamma-methiolbutyrate
-
-
?
S-(1,1,2,2-tetrafluoroethyl)-L-cysteine + 4-(methylsulfanyl)-2-oxobutanoate
?
show the reaction diagram
-
about 63% the rate exhibited with L-phenylalanine and alpha-oxo-gamma-methiolbutyrate
-
-
?
S-(1,2-dichlorovinyl)-L-cysteine + 4-(methylsulfanyl)-2-oxobutanoate
?
show the reaction diagram
-
about 13% the rate exhibited with L-phenylalanine and alpha-oxo-gamma-methiolbutyrate
-
-
?
S-aminoethyl-L-cysteine + phenylpyruvate
2H-1,4-thiazine-5,6-dihydro-3-carboxylic acid + L-phenylalanine
show the reaction diagram
-
-
-
r
S-aminoethyl-L-cysteine + phenylpyruvate
S-aminoethyl-(3-mercapto-2-oxopropanoate) + L-phenylalanine
show the reaction diagram
-
-
-
-
r
S-carbamoyl-L-cysteine + glyoxylate
S-carbamoyl-3-mercapto-2-oxo-propanoate + glycine
show the reaction diagram
-
-
-
-
r
S-methyl-L-cysteine + glyoxylate
3-methylsulfanyl-2-oxopropanoate + glycine
show the reaction diagram
-
-
-
-
r
S-methyl-L-cysteine + phenylpyruvate
3-methylsulfanyl-2-oxopropanoate + L-phenylalanine
show the reaction diagram
-
-
-
-
r
Se-methyl-L-selenocysteine + 4-(methylsulfanyl)-2-oxobutanoate
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-glutamine + phenylpyruvate
2-oxoglutaramate + L-phenylalanine
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
amino-oxyacetic acid
-
-
CH3-S-Hg-homocysteine
-
50% inhibition at 0.5 mM
CH3Hg-S-Cys
-
substrate and reversible inhibitor, 50% inhibition at 0.5 mM
Cys-S-Hg-S-Cys
-
substrate and reversible inhibitor, 37% inhibition at 0.5 mM
glutamine
-
competitive inhibition of the histidine-pyruvate aminotransferase activity
homocysteine-S-Hg-S-homocysteine
-
67% inhibition at 1 mM
hydroxylamine
Isonicotinic acid hydrazide
p-chloromercuribenzoate
-
0.2 mM, 92% inhibition
Se(4-chlorophenyl)-L-selenocysteine
-
substrate inhibition above 0.5 mM
Se(4-methylphenyl)-L-selenocysteine
-
substrate inhibition above 0.5 mM
Semicarbazide
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.9
(-)Allocystathionine
-
pH 9.0, 37C
0.25
2-oxo-4-methiolbutyrate
-
pH 7.0, 30C, L-glutamine as amino group donor
25
2-oxoadipate
-
pH 8.0, 25C
9.19
2-oxobutyrate
-
pH 7.0, 30C, L-glutamine as amino group donor
0.1 - 20.5
2-Oxoglutaramate
39
2-oxoglutarate
-
pH 8.0, 25C
6.76
2-oxovalerate
-
pH 7.0, 30C, L-glutamine as amino group donor
0.61
3-Mercaptopyruvate
-
pH 7.0, 30C, L-glutamine as amino group donor
0.14
4-hydroxyphenylpyruvate
-
pH 8.0, 25C
0.92
alpha-keto-gamma-methiolbutyrate
-
pH 8.5, 37C, L-phenylalanine as amino group donor
8.7
alpha-keto-n-caproate
-
pH 8.5, 37C, L-phenylalanine as amino group donor
7.8
alpha-keto-n-valerate
-
pH 8.5, 37C, L-phenylalanine as amino group donor
18
alpha-Ketobutyrate
-
pH 8.5, 37C
1.4
cystine
-
pH 9.0, 37C
1.46
glyoxalate
-
pH 7.0, 30C, L-glutamine as amino group donor
1.5 - 4.8
glyoxylate
0.16
Indolepyruvate
-
pH 8.0, 25C
6.91
L-5-N-Methylglutamine
-
pH 7.0, 30C
63
L-alanine
-
pH 8.0, 25C
130
L-arginine
-
pH 8.0, 25C
1
L-asparagine
-
pH 8.0, 25C
19
L-aspartate
-
pH 8.0, 25C
1.9
L-cystathionine
-
pH 9.0, 37C
2.53 - 4.4
L-ethionine
21
L-glutamate
-
pH 8.0, 25C
96
L-glutamate-5-ethylester
-
pH 7.0, 30C
16.5
L-glutamate-5-methylester
-
pH 7.0, 30C
0.6 - 3.5
L-glutamine
18
L-histidine
-
pH 8.0, 25C
3.2
L-kynurenine
-
pH 8.0, 25C
180
L-leucine
-
pH 8.0, 25C
1.96 - 4.2
L-methionine
2.15
L-methionine(SR)sulfoxide
-
pH 7.0, 30C
0.092 - 0.76
L-phenylalanine
2.85
L-S-methylcysteine
-
pH 7.0, 30C
37
L-serine
-
pH 8.0, 25C
55
L-threonine
-
pH 8.0, 25C
6
L-tryptophan
-
pH 8.0, 25C
0.82
L-tyrosine
-
pH 8.0, 25C
5
Lanthionine
-
pH 9.0, 37C
0.023 - 0.13
phenylpyruvate
0.06 - 27
pyruvate
1
S-aminomethyl-L-cysteine
-
pH 9.0, 37C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.1
2-oxoglutarate
Thermus thermophilus
-
pH 8.0, 25C
620
4-hydroxyphenylpyruvate
480
Indolepyruvate
Thermus thermophilus
-
pH 8.0, 25C
0.18
L-alanine
Thermus thermophilus
-
pH 8.0, 25C
0.12
L-arginine
Thermus thermophilus
-
pH 8.0, 25C
0.13
L-asparagine
Thermus thermophilus
-
pH 8.0, 25C
0.016
L-aspartate
Thermus thermophilus
-
pH 8.0, 25C
0.03
L-glutamate
Thermus thermophilus
-
pH 8.0, 25C
830
L-glutamine
Thermus thermophilus
-
pH 8.0, 25C
1.3
L-histidine
Thermus thermophilus
-
pH 8.0, 25C
1100
L-kynurenine
Thermus thermophilus
-
pH 8.0, 25C
1.5
L-leucine
Thermus thermophilus
-
pH 8.0, 25C
1200
L-methionine
Thermus thermophilus
-
pH 8.0, 25C
590
L-phenylalanine
Thermus thermophilus
-
pH 8.0, 25C
1.2
L-serine
Thermus thermophilus
-
pH 8.0, 25C
0.013
L-threonine
Thermus thermophilus
-
pH 8.0, 25C
990
L-tryptophan
Thermus thermophilus
-
pH 8.0, 25C
970
L-tyrosine
Thermus thermophilus
-
pH 8.0, 25C
130
phenylpyruvate
Thermus thermophilus
-
pH 8.0, 25C
15
pyruvate
Thermus thermophilus
-
pH 8.0, 25C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.15
-
recombinant cytosolic isozyme, expressed in COS1 cells
0.36
-
histidine-pyruvate activity
3.3
-
glutamine-phenylpyruvate activity
3.68
-
cytoplasmic isozyme
4.75
-
mitochondrial isozyme
6.48
-
cytoplasmic isozyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
-
S-aminoethyl-L-cysteine as substrate
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.2 - 10
-
about half-maximal activity at pH 8.2 and 10.0
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2
-
pH 9.0, 37C, isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
82000
-
gel filtration
97000
-
PAGE
98000
-
gel filtration
100000
-
gel filtration
110000
-
kidney enzyme, gel filtration
300000
-
cytosolic isozyme with beta-elimination activity, gel filtration
330000
-
cytosolic isozyme with beta-elimination activity, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native form, in complex with 3-phenylpropionate and in complex with 2-keto-gamma-methylthiobutanoate
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-oxo acids protect against heat denaturation
-
rather unstable in concentrated ammonium sulfate solution
-
repeated freeze-thawing causes irreversible inactivation
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 30 mM phosphate buffer, pH 7.0, 1 mM mercaptoethanol, 1 mM EDTA, stable for at least 1 month
-
4C, both isozymes stored in 200 mM potassium phosphate buffer, pH 7.2, 20% glycerol and 5 mM 2-mercaptoethanol, stable for at least 2 months
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cDNA clone encoding KAT/GTK, transfection of HEK-293 cells
-
cytosolic kidney isozyme cloned and sequenced, full-length clone expressed in COS1 cells
-
Escherichia coli K 12 genomic DNA PCR amplified , pET22b, expressed in transformed Escherichia coli BL21(DE3)pLysS
-