Information on EC 2.6.1.71 - lysine-pyruvate 6-transaminase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.6.1.71
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RECOMMENDED NAME
GeneOntology No.
lysine-pyruvate 6-transaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-lysine + pyruvate = (S)-2-amino-6-oxohexanoate + L-alanine
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-lysine degradation IX
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SYSTEMATIC NAME
IUBMB Comments
L-lysine:pyruvate aminotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
114189-79-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain H17
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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growth on L-lysine as a nitrogen source can be enhanced when the aruH gene encoding an arginine/lysine:pyruvate transaminase is expressed constitutively from plasmids. The AruH gene product cannot substķtute for loss of lysine decarboxylase A, indicating a minor role of this transaminase in L-lysine catabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-amino-2-oxobutyrate + pyruvate
2,4-dioxobutanoate + L-alanine
show the reaction diagram
L-lysine + pyruvate
2-aminoadipate 6-semialdehyde + L-alanine
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-lysine + pyruvate
2-aminoadipate 6-semialdehyde + L-alanine
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CaCl2
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80% remaining activity at 1 mM, 7% remaining activity at 10 mM
CoCl2
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9% activity retained at 1 mM, complete inhibition at 10 mM
CuCl2
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complete inhibition at 1 mM or higher
EDTA
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89% activity retained at 1 mM, 36% activity retained at 10 mM
FeCl2
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23% activity retained at 1 mM, 13% activity retained at 10 mM
HgCl2
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40% activity retained at 1 mM, 31% activity retained at 10 mM
hydroxylamine
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37% activity retained at 1 mM, 6% activity retained at 10 mM
MgCl2
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98% activity retained at 1 mM, 22% activity retained at 10 mM
MnCl2
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57% activity retained at 1 mM, 3% activity retained at 10 mM
p-chloromercuribenzoate
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5% activity retained at 1 mM, 1% activity retained at 10 mM
SnCl2
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97% activity retained at 1 mM, 40% retained activity at 10 mM
additional information
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KF, KCN, sodium azide, Na2HAsSO4, NaHSO4, 2-mercaptoethanol, dithioerythritol, reduced glutathione, proteinogenic amino acids, none of them has inhibitory effects even at 10 mM
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 30
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activity increases linearly with temperature
15 - 45
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50% of maximal activity at 15°C and 45°C
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
90000
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gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
centrifugation and DEAE-cellulose, partial purification
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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growth on L-lysine as a nitrogen source can be enhanced when the aruH gene encoding an arginine/lysine:pyruvate transaminase is expressed constitutively from plasmids