Information on EC 2.6.1.80 - nicotianamine aminotransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.6.1.80
-
RECOMMENDED NAME
GeneOntology No.
nicotianamine aminotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
nicotianamine + 2-oxoglutarate = 3''-deamino-3''-oxonicotianamine + L-glutamate
show the reaction diagram
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
2'-deoxymugineic acid phytosiderophore biosynthesis
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-
SYSTEMATIC NAME
IUBMB Comments
nicotianamine:2-oxoglutarate aminotransferase
A pyridoxal-phosphate protein. This enzyme is produced by grasses. They secrete both the nicotianamine and the transaminated product into the soil around them. Both compounds chelate iron(II) and iron(III); these chelators, called mugineic acid family phytosiderophores, are taken up by the grass, which is thereby supplied with iron.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
nicotianamine + 2-oxoglutarate
3''-deamino-3''-oxonicotianamine + L-glutamate
show the reaction diagram
additional information
?
-
-
a point mutation in a gene encoding nicotianamine aminotransferase (NAAT1) stimulates the Fe(II) acquisition system and leads to iron accumulation in rice
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
nicotianamine + 2-oxoglutarate
3''-deamino-3''-oxonicotianamine + L-glutamate
show the reaction diagram
additional information
?
-
-
a point mutation in a gene encoding nicotianamine aminotransferase (NAAT1) stimulates the Fe(II) acquisition system and leads to iron accumulation in rice
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-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4 - 0.9
nicotianamine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0005
-
-
0.00088
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-
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8
deduced from nucleotide sequence
6.8
deduced from nucleotide sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
OsNAAT1 is induced in all the cells of Fe-deficient leaves
Manually annotated by BRENDA team
OsNAAT1, but not OsNAAT2-6, is strongly up-regulated by Fe deficiency, both in roots and shoots; OsNAAT1, but not OsNAAT2-6, is strongly up-regulated by Fe deficiency, both in roots and shoots; OsNAAT1, but not OsNAAT2-6, is strongly up-regulated by Fe deficiency, both in roots and shoots; OsNAAT1, but not OsNAAT2-6, is strongly up-regulated by Fe deficiency, both in roots and shoots. OsNAAT1 expression is observed in the companion cells of Fe-sufficient shoots
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80000
-
gel filtration
90000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 46600, deduced from nucleotide sequence; x * 46600, deduced from nucleotide sequence
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Butyl Toyopearl 650M, hydroxylapatite, DEAE-Sephacel
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Butyl toyopearl, hydroxylapatite, DEAE-Sepharose; Butyl toyopearl, hydroxylapatite, DEAE-Sepharose; Butyl toyopearl, hydroxylapatite, DEAE-Sepharose, NA-Sepharose
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Oryza sativa; expression in Oryza sativa
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expression in Saccharomyces cerevisiae; expression in Saccharomyces cerevisiae