Information on EC 2.7.1.170 - anhydro-N-acetylmuramic acid kinase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.170
-
RECOMMENDED NAME
GeneOntology No.
anhydro-N-acetylmuramic acid kinase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + 1,6-anhydro-N-acetyl-beta-muramate + H2O = ADP + N-acetylmuramate 6-phosphate
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
anhydromuropeptides recycling
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:1,6-anhydro-N-acetyl-beta-muramate 6-phosphotransferase
This enzyme, along with EC 4.2.1.126, N-acetylmuramic acid 6-phosphate etherase, is required for the utilization of anhydro-N-acetylmuramic acid in proteobacteria. The substrate is either imported from the medium or derived from the bacterium's own cell wall murein during cell wall recycling. The product N-acetylmuramate 6-phosphate is produced as a 7:1 mixture of the alpha- and beta-anomers.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
MurQ and AnmK kinase are required for utilization of 1,6-anhydro-N-acetyl-beta-muramate derived either from cell wall murein or imported from the medium
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 1,6-anhydro-N-acetyl-beta-muramate + H2O
ADP + N-acetyl-beta-muramate 6-phosphate + H+
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ADP
-
strong inhibitor of AnmK
additional information
-
AnmK is not inhibited by N-acetyl-beta-muramate 6-phosphate
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2 - 1
1,6-anhydro-N-acetyl-beta-muramate
1
ATP
-
in 100 mM Tris-HCl (pH 7.5), 20 mM MgCl2, at 37C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4
ADP
-
in 100 mM Tris-HCl (pH 7.5), 20 mM MgCl2, at 37C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
-
from no activity at pH 4.5 and 5% of the maximum activity at pH 6.0, the activity continues to increase to pH 10.0
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
39500
-
calculated from amino acid sequence
40000
-
SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
x-ray crystallography
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
AnmK in complex with 1,6-anhydro-N-acetylmuramic acid and ADP, hanging drop vapor diffusion method, using 25% (w/v) polyethylene glycol 4000, 0.2 M (NH4)2SO4, and 0.1 M tri-sodium citrate (pH 5.6)
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE-Sephacel column chromatography, hydroxylapatite column chromatography, and Mono Q column chromatography
-
Ni-NTA-agarose column chromatography and gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3) Gold cells
Show AA Sequence (4737 entries)
Please use the Sequence Search for a certain query.