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ADP + 5'-HO-AUCACGCUUpCp
?
-
-
-
r
ATP + (AG)10GGCCC-fluorescein
?
Tequatrovirus T4
-
-
-
?
ATP + (AG)10GGCCC-tetramethylrhodamine
?
Tequatrovirus T4
-
-
-
?
ATP + 2'(3')-ribonucleotides
?
ATP + 3'-carboxyfluorescein-labeled DNA/complementary DNA hybrid
?
Tequatrovirus T4
-
-
-
-
?
ATP + 5'-CACTGTAACTGATCCTGCCGCTATG-3'
?
Tequatrovirus T4
-
-
-
-
?
ATP + 5'-CATAGCGGCAGGATCAGTTACAGTG-3'
?
Tequatrovirus T4
-
-
-
-
?
ATP + 5'-CCTAACCCTTTCTTTCTTTTCAGGGTTAGGGTTAGGGTTAGGG-3'
?
Tequatrovirus T4
-
-
-
-
?
ATP + 5'-CGAGGCTGCACT-BHQ2-3'
ADP + 5'-phospho-CGAGGCTGCACT-BHQ2-3'
Tequatrovirus T4
-
-
-
-
?
ATP + 5'-CTAGAGCTACAATTGCGACCG-3'
ADP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3'
ATP + 5'-CTGGCGCTTGATGGTATTTTTACCATCAAGCGCCAG-3'
?
Tequatrovirus T4
-
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
ATP + 5'-dephospho-GAAAA-RNA
ADP + 5'-phospho-GAAAA-RNA
-
-
-
?
ATP + 5'-dephospho-GC-RNA
ADP + 5'-phospho-GC-RNA
-
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
ATP + 5'-dsRNA
ADP + 5'-phospho-dsRNA
-
-
-
-
?
ATP + 5'-GGCAACAT
?
Tequatrovirus T4
-
-
-
-
?
ATP + 5'-HO-CCGACCAACGAAGGT
?
-
-
-
r
ATP + 5'-hydroxyl poly(A)
?
ATP + 5'-hydroxyl poly(C)
?
-
-
-
-
?
ATP + 5'-OH DNA
ADP + 5'-phospho-DNA
-
-
-
r
ATP + 5'-OH RNA
ADP + 5'-phospho-RNA
preference for RNA substrates
-
-
r
ATP + d(A20)
ADP + 5'-phospho-d(A20)
-
-
-
-
?
ATP + deoxynucleoside 3'-phosphate
ADP + deoxynucleoside 3',5'-diphosphate
Tequatrovirus T4
-
-
-
-
?
ATP + GGGCC(AG)10GGCCC-fluorescein
?
Tequatrovirus T4
-
-
-
?
ATP + GGGCC(AG)10GGCCC-tetramethylrhodamine
?
Tequatrovirus T4
-
-
-
?
ATP + GGGCC(AG)12GGCCC-fluorescein
?
Tequatrovirus T4
-
-
-
?
ATP + GGGCC(AG)8GGCCC-fluorescein
?
Tequatrovirus T4
-
-
-
?
ATP + GGGGC(AG)10GCCCC-fluorescein
?
Tequatrovirus T4
-
-
-
?
ATP + GGGGC(AG)10GCCCC-tetramethylrhodamine
?
Tequatrovirus T4
-
-
-
?
ATP + GGGGG(AG)10CCCCC-fluorescein
?
Tequatrovirus T4
-
-
-
?
ATP + GGGGG(AG)10CCCCC-tetramethylrhodamine
?
Tequatrovirus T4
-
-
-
?
ATP + magnetite microspheres coated with TiO2-DNA complex
?
Tequatrovirus T4
-
-
-
-
?
ATP + nucleoside-3'-monophosphate
ADP + nucleoside-3',5'-diphosphate
ATP + oligo (dT)25
?
-
-
-
-
?
ATP + oligonucleotides
ADP + oligonucleotide 5'-phosphate
Tequatrovirus T4
-
-
-
-
?
ATP + r(A20)
ADP + 5'-phospho-r(A20)
-
-
-
-
?
ATP + swing arm I
ADP + 5'-phospho-swing arm I
Tequatrovirus T4
-
-
-
-
?
ATP + synthetic oligonucleotide
ADP + oligonucleotide 5'-phosphate
beta,gamma-imidoadenylyl 5'-triphosphate + 5'-phospho-DNA
beta,gamma-imidoadenylyl 5'-tetraphosphate + 5'-dephospho-DNA
CTP + 5'-CTAGAGCTACAATTGCGACCG-3'
CDP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3'
CTP + 5'-dephospho-DNA
CDP + 5'-phospho-DNA
CTP + 5'-dephospho-RNA
CDP + 5'-phospho-RNA
dATP + 5'-CTAGAGCTACAATTGCGACCG-3'
dADP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3'
dATP + 5'-dephospho-DNA
dADP + 5'-phospho-DNA
Tequatrovirus T4
-
-
-
?
dATP + 5'-dephospho-RNA
dADP + 5'-phospho-RNA
-
-
-
r
GTP + 5'-CTAGAGCTACAATTGCGACCG-3'
GDP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3'
-
-
-
-
?
GTP + 5'-dephospho-DNA
GDP + 5'-phospho-DNA
GTP + 5'-dephospho-RNA
GDP + 5'-phospho-RNA
-
-
-
r
TTP + 5'-dephospho-DNA
TDP + 5'-phospho-DNA
Tequatrovirus T4
-
-
-
?
UTP + 5'-CTAGAGCTACAATTGCGACCG-3'
UDP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3'
-
-
-
-
?
UTP + 5'-dephospho-DNA
UDP + 5'-phospho-DNA
UTP + 5'-dephospho-RNA
UDP + 5'-phospho-RNA
-
-
-
r
[gamma-S]ATP + 5'-dephospho-RNA
[gamma-S]ADP + 5'-phospho-RNA
-
-
-
?
[gamma-S]ATP + 5'-OH-(ribonucleotide)7
[gamma-S]ADP + 5'-phospho-(ribonucleotide)7
[gamma-S]GTP + 5'-dephospho-RNA
[gamma-S]GDP + 5'-phospho-RNA
additional information
?
-
ATP + 2'(3')-ribonucleotides
?
-
no activity with 2'(3')-AMP and 2'(3')-CMP
-
-
?
ATP + 2'(3')-ribonucleotides
?
-
no activity with (2')-AMP
-
-
?
ATP + 3'-CMP
ADP + pCp
Kostyavirus CJW1
-
-
-
-
?
ATP + 3'-CMP
ADP + pCp
Omegavirus omega
-
-
-
-
?
ATP + 5'-CTAGAGCTACAATTGCGACCG-3'
ADP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3'
-
-
-
-
?
ATP + 5'-CTAGAGCTACAATTGCGACCG-3'
ADP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3'
-
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
substrate is a synthetic 36-mer 5'-OH DNA oligonucleotide d(CCTGTTCTTATTGGCCTCCTGGCATACCTTTTCCGG)
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
the enzyme makes hydrogen bonds to the ribose 2'-hydroxyls of the 5'-terminal nucleoside, via Gln51, and the penultimate nucleoside, via Gln83
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
the enzyme makes hydrogen bonds to the ribose 2'-hydroxyls of the 5'-terminal nucleoside, via Gln51, and the penultimate nucleoside, via Gln83
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
double stranded DNA
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
double stranded DNA
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
minimal length of the substrate is 7-8 nucleotides, optimal size is more than 18 nucleotides in length
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
no preference for overhanging 5'-ends
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
single stranded DNA
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
single stranded DNA
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
single stranded DNA
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
specific for DNA
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
micrococcal-nuclease-treated calf thymus DNA
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
involved in repair of DNA single strand breaks
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
micrococcal-nuclease-treated calf thymus DNA
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
-
r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
involved in repair of DNA single strand breaks
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Kostyavirus CJW1
-
37-mer oligodeoxyribonucleotide substrate
-
-
r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
preference for recessed termini within duplex DNA
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
no preference for overhanging 5'-ends
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
involved in repair of chromosomal DNA strand breaks that arise continously, but at low frequency, and are potentially lethal
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
low activity
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
micrococcal-nuclease-treated calf thymus DNA is not effectively phosphorylated
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Omegavirus omega
-
37-mer oligodeoxyribonucleotide substrate
-
-
r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
-
r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
double stranded DNA
-
?, r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
double stranded DNA
UDP, ADP, GDP and CDP support the reverse reaction
r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
minimal length of the substrate is 7-8 nucleotides, optimal size is more than 18 nucleotides in length
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
nuclease-treated calf thymus DNA
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
large DNA fragments released by nuclease treatment
-
?, r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
large DNA fragments released by nuclease treatment
UDP, ADP, GDP and CDP support the reverse reaction
r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
single stranded DNA
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
single stranded DNA
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
single stranded DNA
-
r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
single stranded DNA
UDP, ADP, GDP and CDP support the reverse reaction
r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
native and denatured DNA are substrates for the reverse reaction
reverse reaction is promoted by a variety of other nucleoside diphosphates but not by ATP
?, r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
native and denatured DNA are substrates for the reverse reaction
native and heat denatured DNA serve as substrates for the reverse reaction
?, r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
specific for DNA
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
native and heat denatured DNA
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
native and heat denatured DNA
UDP, ADP, GDP and CDP support the reverse reaction
r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
acts both on single- and blunt end double-stranded DNA
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
-
r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
-
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
-
-
730493, 737431, 737436, 737441, 737443, 737444, 737575, 737888, 737889, 737890, 760338 -
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
-
-
-
r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
-
-
-
-
r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
-
-
-
r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
-
-
-
-
r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
-
double stranded DNA
-
r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
-
double stranded DNA
excess ADP will cause the reverse reaction to be favored
?, r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
-
large DNA fragments released by nuclease treatment
excess ADP will cause the reverse reaction to be favored
?, r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
-
single stranded DNA
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
-
single stranded DNA
excess ADP will cause the reverse reaction to be favored
?, r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
-
preference for single stranded termini due to the narrow tunnel formation of the active site that can only bind slim molecules
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
-
micrococcal-nuclease-treated calf thymus DNA
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
-
micrococcal-nuclease-treated calf thymus DNA
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4 K10
-
-
-
-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
-
-
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
-
-
-
r
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
-
no activity
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
-
low activity
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
-
phosphorylated at a much lower rate than DNA
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
-
-
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
-
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
-
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
-
soluble and ribosomal RNA of Escherichia coli
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
-
synthetic polynucleotides
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
-
no activity
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
-
much more efficient as substrate than DNA
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
-
no activity
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
-
much more efficient as substrate than DNA
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
-
no activity
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
-
enzyme cannot act on RNA less than 10 bases in length
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
-
solely RNA-specific
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
-
5'-HO-tRNA is a very poor substrate
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
-
-
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
-
-
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
Tequatrovirus T4
-
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
Tequatrovirus T4
-
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
Tequatrovirus T4
-
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
Tequatrovirus T4
-
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
Tequatrovirus T4
-
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
Tequatrovirus T4
-
-
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
Tequatrovirus T4
-
involved in repair of broken RNA termini
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
Tequatrovirus T4
-
involved in repair of broken RNA termini
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
Tequatrovirus T4 K10
-
-
-
-
?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
-
-
-
-
?
ATP + 5'-hydroxyl poly(A)
?
-
-
-
-
?
ATP + 5'-hydroxyl poly(A)
?
-
-
-
-
?
ATP + nucleoside-3'-monophosphate
ADP + nucleoside-3',5'-diphosphate
-
no activity with thymidine 3'-monophosphate
-
-
?
ATP + nucleoside-3'-monophosphate
ADP + nucleoside-3',5'-diphosphate
-
-
-
-
?
ATP + nucleoside-3'-monophosphate
ADP + nucleoside-3',5'-diphosphate
-
-
-
-
?
ATP + nucleoside-3'-monophosphate
ADP + nucleoside-3',5'-diphosphate
-
no activity
-
-
?
ATP + nucleoside-3'-monophosphate
ADP + nucleoside-3',5'-diphosphate
Tequatrovirus T4
-
-
-
-
?
ATP + synthetic oligonucleotide
ADP + oligonucleotide 5'-phosphate
-
-
-
-
?
ATP + synthetic oligonucleotide
ADP + oligonucleotide 5'-phosphate
-
low activity with homopolymers such as oligo(dA)24 and oligo(dT)24
-
-
?
ATP + synthetic oligonucleotide
ADP + oligonucleotide 5'-phosphate
-
no activity with poly(A)
-
-
?
ATP + synthetic oligonucleotide
ADP + oligonucleotide 5'-phosphate
-
oligo (dT)25
-
-
?
ATP + synthetic oligonucleotide
ADP + oligonucleotide 5'-phosphate
-
-
-
-
?
ATP + synthetic oligonucleotide
ADP + oligonucleotide 5'-phosphate
-
5'-hydroxyl poly(A)
-
-
?
ATP + synthetic oligonucleotide
ADP + oligonucleotide 5'-phosphate
-
5'-hydroxyl poly(C)
-
-
?
ATP + synthetic oligonucleotide
ADP + oligonucleotide 5'-phosphate
-
low activity with 5'-hydroxyl poly(I)
-
-
?
ATP + synthetic oligonucleotide
ADP + oligonucleotide 5'-phosphate
-
5'-hydroxyl poly(dA), at 6% of 5'-hydroxyl poly(A)
-
-
?
ATP + synthetic oligonucleotide
ADP + oligonucleotide 5'-phosphate
-
5'-hydroxyl poly(A)
-
-
?
ATP + synthetic oligonucleotide
ADP + oligonucleotide 5'-phosphate
-
-
-
-
?
ATP + synthetic oligonucleotide
ADP + oligonucleotide 5'-phosphate
-
(dT)10
-
-
?
ATP + synthetic oligonucleotide
ADP + oligonucleotide 5'-phosphate
-
no activity with low molecular weight oligonucleotides
-
-
?
ATP + synthetic oligonucleotide
ADP + oligonucleotide 5'-phosphate
-
oligodeoxynucleotides of chain length above 10-12 residues
-
-
?
ATP + synthetic oligonucleotide
ADP + oligonucleotide 5'-phosphate
synthetic ribozyme
-
substrate 5'-OH-(ribonucleotide)7
-
-
?
ATP + synthetic oligonucleotide
ADP + oligonucleotide 5'-phosphate
Tequatrovirus T4
-
-
-
-
?
ATP + synthetic oligonucleotide
ADP + oligonucleotide 5'-phosphate
Tequatrovirus T4
-
oligo dpT(pT)9
-
-
?
ATP + synthetic oligonucleotide
ADP + oligonucleotide 5'-phosphate
Tequatrovirus T4
-
oligodeoxynucleotides of chain length less than approximately 10-12 residues
-
-
?
beta,gamma-imidoadenylyl 5'-triphosphate + 5'-phospho-DNA
beta,gamma-imidoadenylyl 5'-tetraphosphate + 5'-dephospho-DNA
Tequatrovirus T4
-
ATP analog, inhibitory, competitive against ATP
-
-
ir
beta,gamma-imidoadenylyl 5'-triphosphate + 5'-phospho-DNA
beta,gamma-imidoadenylyl 5'-tetraphosphate + 5'-dephospho-DNA
Tequatrovirus T4
-
is no substrate in the forward reaction but can replace ADP and ATP in the reverse reaction
-
-
ir
CTP + 5'-CTAGAGCTACAATTGCGACCG-3'
CDP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3'
-
-
-
-
?
CTP + 5'-CTAGAGCTACAATTGCGACCG-3'
CDP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3'
-
-
-
-
?
CTP + 5'-dephospho-DNA
CDP + 5'-phospho-DNA
-
-
-
?
CTP + 5'-dephospho-DNA
CDP + 5'-phospho-DNA
-
-
-
?
CTP + 5'-dephospho-DNA
CDP + 5'-phospho-DNA
-
-
-
?
CTP + 5'-dephospho-DNA
CDP + 5'-phospho-DNA
-
-
-
?
CTP + 5'-dephospho-DNA
CDP + 5'-phospho-DNA
-
CTP gives 15% of the activity with ATP
-
?
CTP + 5'-dephospho-DNA
CDP + 5'-phospho-DNA
-
-
-
?
CTP + 5'-dephospho-DNA
CDP + 5'-phospho-DNA
-
-
-
?
CTP + 5'-dephospho-DNA
CDP + 5'-phospho-DNA
-
UDP, ADP, GDP and CDP support the reverse reaction
-
r
CTP + 5'-dephospho-RNA
CDP + 5'-phospho-RNA
-
-
-
r
CTP + 5'-dephospho-RNA
CDP + 5'-phospho-RNA
-
less effective than ATP
-
?
dATP + 5'-CTAGAGCTACAATTGCGACCG-3'
dADP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3'
-
-
-
-
?
dATP + 5'-CTAGAGCTACAATTGCGACCG-3'
dADP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3'
-
-
-
-
?
GTP + 5'-dephospho-DNA
GDP + 5'-phospho-DNA
-
-
-
?
GTP + 5'-dephospho-DNA
GDP + 5'-phospho-DNA
-
-
-
?
GTP + 5'-dephospho-DNA
GDP + 5'-phospho-DNA
-
-
-
?
GTP + 5'-dephospho-DNA
GDP + 5'-phospho-DNA
-
-
-
?
GTP + 5'-dephospho-DNA
GDP + 5'-phospho-DNA
-
GTP gives 15% of the activity with ATP
-
?
GTP + 5'-dephospho-DNA
GDP + 5'-phospho-DNA
-
-
-
?
GTP + 5'-dephospho-DNA
GDP + 5'-phospho-DNA
-
-
-
?
GTP + 5'-dephospho-DNA
GDP + 5'-phospho-DNA
-
UDP, ADP, GDP and CDP support the reverse reaction
-
r
GTP + 5'-dephospho-DNA
GDP + 5'-phospho-DNA
-
-
-
-
?
UTP + 5'-dephospho-DNA
UDP + 5'-phospho-DNA
-
-
-
?
UTP + 5'-dephospho-DNA
UDP + 5'-phospho-DNA
-
-
-
?
UTP + 5'-dephospho-DNA
UDP + 5'-phospho-DNA
-
-
-
?
UTP + 5'-dephospho-DNA
UDP + 5'-phospho-DNA
-
UDP, ADP, GDP and CDP support the reverse reaction
-
r
UTP + 5'-dephospho-DNA
UDP + 5'-phospho-DNA
Tequatrovirus T4
-
-
-
?
[gamma-S]ATP + 5'-OH-(ribonucleotide)7
[gamma-S]ADP + 5'-phospho-(ribonucleotide)7
synthetic ribozyme
-
best phosphate donor
-
?
[gamma-S]ATP + 5'-OH-(ribonucleotide)7
[gamma-S]ADP + 5'-phospho-(ribonucleotide)7
synthetic ribozyme
-
very low activity with [gamma-S]GTP
-
?
[gamma-S]GTP + 5'-dephospho-RNA
[gamma-S]GDP + 5'-phospho-RNA
-
less effective than ATP
-
?
[gamma-S]GTP + 5'-dephospho-RNA
[gamma-S]GDP + 5'-phospho-RNA
-
-
-
?
additional information
?
-
Pnkp is a bifunctional enzyme: 2,3' cyclic phosphate and 5-OH ends are substrates for healing and sealing by Pnkp and Hen1, The 5' end is phosphorylated by the Pnkp kinase and the 2',3' cyclic phosphate is removed by the Pnkp phosphatase
-
-
?
additional information
?
-
-
Pnkp is a bifunctional enzyme: 2,3' cyclic phosphate and 5-OH ends are substrates for healing and sealing by Pnkp and Hen1, The 5' end is phosphorylated by the Pnkp kinase and the 2',3' cyclic phosphate is removed by the Pnkp phosphatase
-
-
?
additional information
?
-
the alpha and beta phosphates are engaged by a network of hydrogen bonds from Thr23 and the P-loop main-chain amides, the gamma phosphate is anchored by the lid residues Arg120 and Arg123. The P-loop lysine (Lys21) and the catalytic Mg2+ bridge the ATP beta and gamma phosphates
-
-
?
additional information
?
-
-
the alpha and beta phosphates are engaged by a network of hydrogen bonds from Thr23 and the P-loop main-chain amides, the gamma phosphate is anchored by the lid residues Arg120 and Arg123. The P-loop lysine (Lys21) and the catalytic Mg2+ bridge the ATP beta and gamma phosphates
-
-
?
additional information
?
-
-
substrate specificity
-
-
?
additional information
?
-
-
substrate specificity
-
-
?
additional information
?
-
-
no activity with thymidine 3'-monophosphate
-
-
?
additional information
?
-
-
enzyme also has DNA 3'-phosphatase activity
-
-
?
additional information
?
-
-
enzyme also has DNA 3'-phosphatase activity
-
-
?
additional information
?
-
-
no 3'-phosphatase activity
-
-
?
additional information
?
-
the enzyme displays no significant activity on mononucleotides
-
-
?
additional information
?
-
-
the enzyme displays no significant activity on mononucleotides
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
substrate specificity
-
-
?
additional information
?
-
-
no activity with adenosine, nucleotides, and 5'-mononucleotides
-
-
?
additional information
?
-
-
enzyme also has DNA 3'-phosphatase activity
-
-
?
additional information
?
-
-
enzyme also has DNA 3'-phosphatase activity
-
-
?
additional information
?
-
-
PALF nuclease activity acts on single-stranded DNA or overhangs of duplex substrates. PALF does not open DNA hairpins. Reduction of PALF in vivo reduces the joining of incompatible DNA ends, PALF can function in concert with other nonhomologous DNA end joining proteins. PALF is able to resect 3 overhanging nucleotides and permit XRCC4-DNA ligase IV to complete the joining process in a manner that is as efficient as Artemis. Role for polynucleotide kinase and aprataxin-like forkhead-associated, PALF, in nonhomologous DNA end joining
-
-
?
additional information
?
-
-
PNKP function is modulated by interaction with the DNA repair scaffold proteins XRCC1 and XRCC4, which is mediated by binding of the PNKP forkhead-associated domain to phosphorylated motifs on XRCC1 and XRCC4, overview. Phosphorylation-independent interaction between PNKP and XRCC1 in human cells, since a non-phosphorylated XRCC1S518A/T519A/T523A triple mutant is also bound
-
-
?
additional information
?
-
-
recombinant Nol9 phosphorylates single-stranded and double-stranded RNA and DNA substrates with high efficiency, mainly pre-60S rRNP particles. Nol9 is able to transfer a phosphate to 5' ends of dsRNAs, but cannot phosphorylate 3' termini
-
-
?
additional information
?
-
-
the enzyme plays a role in tRNA splicing. The ATP-binding and/or hydrolysis capacity of CLP1 is required to enhance pre-tRNA cleavage
-
-
?
additional information
?
-
-
enzyme interacts with XRCC1, a scaffold protein that helps recruit repair enzymes at sites of single-strand breakage
-
-
?
additional information
?
-
prefers double-stranded substrates with recessed 5-termini
-
-
?
additional information
?
-
-
PNKP function is modulated by interaction with the DNA repair scaffold proteins XRCC1 and XRCC4, which is mediated by binding of the PNKP forkhead-associated domain to phosphorylated motifs on XRCC1 and XRCC4, overview
-
-
?
additional information
?
-
-
enzyme also has DNA 3'-phosphatase activity
-
-
?
additional information
?
-
-
enzyme also has DNA 3'-phosphatase activity
-
-
?
additional information
?
-
-
enzyme also has DNA 3'-phosphatase activity
-
-
?
additional information
?
-
-
enzyme also has DNA 3'-phosphatase activity
-
-
?
additional information
?
-
-
the enzyme also has RNA 2',3-cyclic phosphodiesterase, DNA 3'-phosphatase and RNA 2'- and 3'-phosphomonoesterase activities
-
-
-
additional information
?
-
-
the enzyme also has RNA 2',3-cyclic phosphodiesterase, DNA 3'-phosphatase and RNA 2'- and 3'-phosphomonoesterase activities
-
-
-
additional information
?
-
-
protein Las1 interacts with the Grc3 polynucleotide kinase
-
-
?
additional information
?
-
synthetic ribozyme
-
substrate specificity
-
-
?
additional information
?
-
synthetic ribozyme
-
binding constants of the reaction steps
-
-
?
additional information
?
-
Tequatrovirus T4
-
-
-
-
?
additional information
?
-
Tequatrovirus T4
-
substrate specificity
-
-
?
additional information
?
-
Tequatrovirus T4
-
enzyme also has DNA 3'-phosphatase activity
-
-
?
additional information
?
-
Tequatrovirus T4
-
enzyme also has DNA 3'-phosphatase activity
-
-
?
additional information
?
-
Tequatrovirus T4
-
enzyme also has DNA 3'-phosphatase activity
-
-
?
additional information
?
-
Tequatrovirus T4
-
poorly active on recessed termini within duplex DNA
-
-
?
additional information
?
-
Tequatrovirus T4
-
in vivo role is possibly in maintaining DNA or RNA in the 5'-phosphorylated 3'-hydroxylated state which is the substrate for many reactions such as ligation and packaging
-
-
?
additional information
?
-
Tequatrovirus T4
-
the enzyme catalyzes both, the phosphorylation of 5-hydroxyl termini and the hydrolysis of 3-phosphomonoesters and 2,3-cyclic phosphodiesters of polynucleotides
-
-
?
additional information
?
-
Tequatrovirus T4
-
the enzyme catalyzes the phosphorylation of the 5'-hydroxyl terminus and the dephosphorylation of the 3'-phosphate terminus of DNA
-
-
?
additional information
?
-
Tequatrovirus T4
-
the enzyme also has RNA 2'-phosphatase activity that requires Asp165 and Asp167
-
-
?
additional information
?
-
Tequatrovirus T4
-
the enzyme shows low activity with ADP, AMP, UTP, GTP, and CTP
-
-
?
additional information
?
-
Tequatrovirus T4 K10
-
the enzyme catalyzes both, the phosphorylation of 5-hydroxyl termini and the hydrolysis of 3-phosphomonoesters and 2,3-cyclic phosphodiesters of polynucleotides
-
-
?
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2-(1-hydroxyundecyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione
2-(hydroxy(3,4,5-trimethoxyphenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione
-
A6B4C3
2-(hydroxy(phenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione
-
A1B4C3
2-(hydroxy(thiophen-2-yl)methyl)-6-methyl-1-(phenylamino)-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione
-
A39B1C2
5'-Hydroxyl poly(I)
-
in combination with 5'-hydroxyl poly(A) or poly(C)
beta,gamma-imidoadenosine 5'-triphosphate
-
binds with high affinity, similar to ATP
beta,gamma-imidoadenylyl 5'-tetraphosphate
Tequatrovirus T4
-
ATP analog, competitive against ATP, noncompetitive against 5'-OH-DNA, serves as substrate for the reverse reaction only
Ca2+
-
inhibits in combination with MgCl2, stimulates without MgCl2
Cibacron blue F3GA
Tequatrovirus T4
-
chromophore of blue dextran, inhibition is competitive to single stranded DNA, noncompetitive with respect to ATP
CTP
-
more than 90% inhibition at 0.3 mM
dATP
-
more than 95% inhibition at 0.3 mM
dCTP
-
80% inhibition at 0.3 mM
Deoxyribonucleoside triphosphates
-
-
dGTP
-
more than 95% inhibition at 0.3 mM
disodium hydrogen phosphate
Tequatrovirus T4
-
-
dTTP
-
more than 95% inhibition at 0.3 mM
GTP
-
more than 90% inhibition at 0.3 mM
Na2HPO4
Tequatrovirus T4
-
-
Ni2+
-
inhibits in combination with MgCl2, stimulates without MgCl2
p-hydroxymercuribenzoate
-
2-mercaptoethanol prevents inhibition
PEG 6000
-
5-15%, 3-4fold increase in activity, inhibitory above
Ribonucleoside 3'-phosphates
-
weak
Ribonucleoside triphosphates
-
-
spermine
-
1 mM enhances activity 3times, inhibition above 1 mM
sulfhydryl antagonists
-
-
-
tert-butyl 2-(1-hydroxy-2,2-diphenylethyl)-6-methyl-5,7-dioxo-2,4a,5,6,7,7a-hexahydro-1H-pyrrolo[3,4-b]pyridine-1yl-carbamate
-
A26B11C2
UTP
-
more than 90% inhibition at 0.3 mM
(NH4)2SO4
Tequatrovirus T4
-
-
(NH4)2SO4
Tequatrovirus T4
-
strong inhibition
(NH4)2SO4
Tequatrovirus T4
-
-
2-(1-hydroxyundecyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione
-
A12B4C3
2-(1-hydroxyundecyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione
A12B4C3, noncompetitive inhibition
2-(1-hydroxyundecyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione
A12B4C3
2-(1-hydroxyundecyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione
-
A12B4C3
2-(1-hydroxyundecyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione
Tequatrovirus T4
-
A12B4C3
ADP
-
-
ADP
-
complete inhibition at 1 mM
ADP
synthetic ribozyme
-
-
ADP
Tequatrovirus T4
-
strong inhibition
AgNO3
-
complete inhibition at 0.3 mM
ammonium sulfate
-
about 75% inhibition at 10 mM
ammonium sulfate
-
80% inhibition at 30 mM
ammonium sulfate
-
50% inhibition at 8.5 mM
ammonium sulfate
Tequatrovirus T4
-
-
chloramphenicol
-
inhibits the formation of enzyme in cells cotransfected with bacteriophage and chloramphenicol
chloramphenicol
Tequatrovirus T4
-
inhibits the formation of enzyme in cells cotransfected with bacteriophage and chloramphenicol
Cu2+
-
inhibits in presence of CuCl2
Cu2+
-
inhibits in presence of MgCl2
Cu2+
-
inhibits in presence of MgCl2
Dextran sulfate
-
-
-
Dextran sulfate
-
forward and reverse reaction at similar amounts; strong inhibition, competitive to ATP and DNA
-
Dextran sulfate
-
strong inhibition
-
diphosphate
-
-
diphosphate
-
97% inhibition at 20 mM
diphosphate
-
50% inhibition at 2.2 mM
diphosphate
Tequatrovirus T4
-
-
EDTA
-
complete inhibition at 25 mM
EDTA
Tequatrovirus T4
-
-
heparin
-
-
heparin
-
strong inhibition
heparin
Tequatrovirus T4
-
iodoacetate
-
complete inhibition at 0.3 mM
KCl
-
-
KCl
-
50% inhibition at 71 mM
KCl
-
weak inhibition, 50% at about 0.3 M
KCl
-
inhibitory above 10 mM
KCl
Tequatrovirus T4
-
KCl stimulates at low concentrations, inhibits at high concentrations
Mg2+
Kostyavirus CJW1
-
1-2 mM, maximum activity, inhibitory above
Mg2+
Omegavirus omega
-
1-2 mM, maximum activity, inhibitory above
Mn2+
-
inhibition above 1 mM
Mn2+
-
inhibition above 1 mM
Mn2+
-
maximum activity at 0.01 mM, inhibitory above 0.1 mM
NaCl
-
-
NaCl
-
50% inhibition at 67 mM
NaCl
-
weak inhibition, 50% at about 0.3 M
NaCl
-
maximum activity in presence of 0.1-0.15 M NaCl, higher concentrations inhibit
NaCl
-
inhibitory above 10 mM
NaCl
Tequatrovirus T4
-
stimulates activity towards single stranded substrates, inhibitory with some duplexes
NH4+
-
-
NH4+
-
50% at about 0.3 M; weak inhibition
NH4+
Tequatrovirus T4
-
-
p-chloromercuribenzoate
-
reversible
p-chloromercuribenzoate
-
reversible by 2-mercaptoethanol
p-chloromercuribenzoate
-
-
phosphate
-
-
phosphate
-
50% inhibition at 11 mM
phosphate
Tequatrovirus T4
-
-
sulfate
-
weak
sulfate
-
50% inhibition at 5.7 mM
sulfate
-
competitive to ATP, noncompetitive to DNA, less sensitive in the reverse reaction
Zn2+
-
inhibition in presence of Mg2+
Zn2+
-
inhibition in presence of Mg2+
additional information
-
no inhibition by agar-agar
-
additional information
-
productive engagement of a 3'-phosphate terminus may block access of a 5'-hydroxyl to the kinase active site
-
additional information
-
productive engagement of a 3'-phosphate terminus may block access of a 5'-hydroxyl to the kinase active site
-
additional information
-
no inhibition by chondroitin sulfates A and C, and by dextran
-
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Abortion, Habitual
Natural killer cells and pregnancy outcomes in women with recurrent miscarriage and infertility: a systematic review.
Abortion, Habitual
The "killer cell story" in recurrent miscarriage: Association between activated peripheral lymphocytes and uterine natural killer cells.
Abortion, Habitual
[IMMUNE DISORDERS AND THEIR ROLE IN ABORTION].
Abortion, Spontaneous
Concordance between peripheral and decidual NK cell subsets and killer immunoglobulin-like receptors in women with recurrent spontaneous miscarriages.
Abortion, Spontaneous
Natural killer cells and pregnancy outcomes in women with recurrent miscarriage and infertility: a systematic review.
Abortion, Spontaneous
Peripheral blood NK cells reflect changes in decidual NK cells in women with recurrent miscarriages.
Abortion, Spontaneous
Rapamycin prevents spontaneous abortion by triggering decidual stromal cell autophagy-mediated NK cell residence.
Abortion, Spontaneous
Tim-3 Is Upregulated in NK Cells during Early Pregnancy and Inhibits NK Cytotoxicity toward Trophoblast in Galectin-9 Dependent Pathway.
Acquired Immunodeficiency Syndrome
Mutational analysis defines the 5'-kinase and 3'-phosphatase active sites of T4 polynucleotide kinase.
Adenocarcinoma
Identification of a small molecule inhibitor of the human DNA repair enzyme polynucleotide kinase/phosphatase.
Adenoma
Polynucleotide kinase 3' phosphatase variant, dietary variables and risk of adenoma recurrence in the Polyp Prevention Trial.
Anemia
Vitamin B6 metabolism in anaemic and alcoholic man.
Anemia, Iron-Deficiency
Vitamin B6 metabolism in anaemic and alcoholic man.
Anemia, Sideroblastic
Vitamin B6 metabolism in anaemic and alcoholic man.
Apraxias
Compound Heterozygous Mutations in PNKP Gene in an Iranian Child with Microcephaly, Seizures, and Developmental Delay.
Breast Neoplasms
A potential role for peripheral natural killer cell activity induced by preoperative chemotherapy in breast cancer patients.
Carcinogenesis
Appearance of artefacts when using 32P-postlabelling to investigate DNA adduct formation by bile acids in vitro: lack of evidence for covalent binding.
Carcinoma
Development of a positive-negative selection procedure for gene targeting in fish cells.
Carcinoma
Identification of a small molecule inhibitor of the human DNA repair enzyme polynucleotide kinase/phosphatase.
Carcinoma, Hepatocellular
Analysis of loss of nuclear RNA in azo dye-induced hepatoma by DNA-RNA competitive hybridization.
Diabetes, Gestational
Expression of Natural Cytotoxicity Receptors on and Intracellular Cytokine Production by NK Cells in Women with Gestational Diabetes Mellitus.
Immune System Diseases
The impact of previous live births on peripheral and uterine natural killer cells in patients with recurrent miscarriage.
Infections
Bacteriophage T4 polynucleotide kinase triggers degradation of mRNAs.
Infections
Bacteriophage T4-induced anticodon-loop nuclease detected in a host strain restrictive to RNA ligase mutants.
Infections
Host transfer RNA cleavage and reunion in T4-infected Escherichia coli CTr5x.
Infections
In vitro reconstitution of anticodon nuclease from components encoded by phage T4 and Escherichia coli CTr5X.
Infections
Phage and host genetic determinants of the specific anticodon loop cleavages in bacteriophage T4-infected Escherichia coli CTr5X.
Infections
[Biosynthesis of early enzymes induced by bacteriophage T4]
Infertility
Natural killer cells and pregnancy outcomes in women with recurrent miscarriage and infertility: a systematic review.
Intellectual Disability
Compound Heterozygous Mutations in PNKP Gene in an Iranian Child with Microcephaly, Seizures, and Developmental Delay.
Leukemia
Single prokaryotic cell isolation and total transcript amplification protocol for transcriptomic analysis.
Leukemia, Myeloid, Acute
CBLB ablation with CRISPR/Cas9 enhances cytotoxicity of human placental stem cell-derived NK cells for cancer immunotherapy.
Lymphatic Metastasis
A potential role for peripheral natural killer cell activity induced by preoperative chemotherapy in breast cancer patients.
Lymphatic Metastasis
Immune correlates of the differing pathological and therapeutic effects of neoadjuvant chemotherapy in breast cancer.
Lymphoma
Maternal-iron-deficiency effects on peritoneal macrophage and peritoneal natural-killer-cell cytotoxicity in rat pups.
Microcephaly
A Novel c.968C?>?T homozygous Mutation in the Polynucleotide Kinase 3'?-?Phosphatase Gene Related to the Syndrome of Microcephaly, Seizures, and Developmental Delay.
Microcephaly
Compound Heterozygous Mutations in PNKP Gene in an Iranian Child with Microcephaly, Seizures, and Developmental Delay.
Microcephaly
Microcephalic primordial dwarfism in an Emirati patient with PNKP mutation.
Microphthalmos
Rapamycin prevents spontaneous abortion by triggering decidual stromal cell autophagy-mediated NK cell residence.
Neoplasm Metastasis
A potential role for peripheral natural killer cell activity induced by preoperative chemotherapy in breast cancer patients.
Neoplasm Metastasis
Immune correlates of the differing pathological and therapeutic effects of neoadjuvant chemotherapy in breast cancer.
Neoplasm Metastasis
Immune factors associated with the pathological and therapeutic effects of preoperative chemotherapy in patients with breast cancer.
Neoplasms
A label-free bioluminescent sensor for real-time monitoring polynucleotide kinase activity.
Neoplasms
A potential role for peripheral natural killer cell activity induced by preoperative chemotherapy in breast cancer patients.
Neoplasms
CBLB ablation with CRISPR/Cas9 enhances cytotoxicity of human placental stem cell-derived NK cells for cancer immunotherapy.
Neoplasms
Characterization and ex vivo Expansion of Human Placenta-Derived Natural Killer Cells for Cancer Immunotherapy.
Neoplasms
DNA end-processing enzyme polynucleotide kinase as a potential target in the treatment of cancer.
Neoplasms
Identification of a small molecule inhibitor of the human DNA repair enzyme polynucleotide kinase/phosphatase.
Neoplasms
Immune correlates of the differing pathological and therapeutic effects of neoadjuvant chemotherapy in breast cancer.
Neoplasms
Immune factors associated with the pathological and therapeutic effects of preoperative chemotherapy in patients with breast cancer.
Neoplasms
Maternal-iron-deficiency effects on peritoneal macrophage and peritoneal natural-killer-cell cytotoxicity in rat pups.
Neoplasms
Mimic Peroxidase- and Bi2S3 Nanorod-Based Photoelectrochemical Biosensor for Signal-On Detection of Polynucleotide Kinase.
Neoplasms
Problem-solving test: analysis of DNA damage recognizing proteins in yeast and human cells.
Neoplasms
Renal cell tumors convert natural killer cells to a proangiogenic phenotype.
Neoplasms
Single-Molecule Detection of Polynucleotide Kinase Based on Phosphorylation-Directed Recovery of Fluorescence Quenched by Au Nanoparticles.
Neurodegenerative Diseases
2 new cases of pontocerebellar hypoplasia type 10 identified by whole exome sequencing in a Turkish family.
Pelvic Inflammatory Disease
A novel fluorescence method for activity assay and drug screening of T4 PNK by coupling rGO with ligase reaction.
Polyneuropathies
Genetic assessment and folate receptor autoantibodies in infantile-onset cerebral folate deficiency (CFD) syndrome.
Pregnancy Complications
[ACTIVATION OF PERIPHERAL NATURAL KILLER CELLS IN WOMEN WITH REPEATED EARLY PREGNANCY LOSS].
Respiratory Insufficiency
Prepacked naloxone administration for suspected opioid overdose in the era of illicitly manufactured fentanyl: a retrospective study of regional poison center data.
Seizures
A Novel c.968C?>?T homozygous Mutation in the Polynucleotide Kinase 3'?-?Phosphatase Gene Related to the Syndrome of Microcephaly, Seizures, and Developmental Delay.
Seizures
Compound Heterozygous Mutations in PNKP Gene in an Iranian Child with Microcephaly, Seizures, and Developmental Delay.
Seizures
Microcephalic primordial dwarfism in an Emirati patient with PNKP mutation.
Vaccinia
A nuclease that cuts specifically in the ribosome binding site of some T4 mRNAs.
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malfunction
-
depletion of Nol9 leads to a severe impairment of ribosome biogenesis. Upon Nol9 knockdown, specific maturation defect at the 5' end of the predominant 5.8S short-form rRNA (5.8SS) occur, possibly due to the Nol9 requirement for 5'>3' exonucleolytic trimming
malfunction
-
knockdown of polynucleotide kinase and aprataxin-like forkhead-associated using siRNA reduces rejoining of two incompatible I-SceI-generated DNA ends by 50%
malfunction
-
Pnk1 deletion in fission yeast renders cells sensitive to camptothecin
malfunction
-
PNKP depletion in human cells renders cells sensitive to camptothecin. A small molecule inhibitor of PNKP phosphatase activity enhances the sensitivity of cells to IR and camptothecin. Enzyme mutational defects can cause neurological disorders with various symptoms, e.g. a severe neurological autosomal recessive disease characterized by microcephaly, intractable seizures and developmental delay
malfunction
-
mutations lead to a loss of enzyme interaction with the tRNA splicing endonuclease complex, largely reduced pretRNA cleavage activity, and accumulation of linear tRNA introns. The affected individuals develop severe motor-sensory defects, cortical dysgenesis, and microcephaly
malfunction
-
the lack of CLP1 kinase activity leads to progressive motor neuron loss and accumulation of novel 5' leader-5' exon tRNA fragments
malfunction
-
polynucleotide kinase Grc3 depletion results in rRNA processing defects
metabolism
-
polynucleotide 5-kinase Nol9 is involved in ribosomal RNA processing
metabolism
the enzyme is part of the Pnkp-Hen1 RNA repair pathway, overview
metabolism
-
the enzyme is required for 60S ribosomal particles synthesis
physiological function
-
bifunctional polynucleotide kinase/phosphatase contains both DNA 5'-kinase and 3'-phosphatase activities required for restoration of 3'-hydroxyls and 5'-phosphates needed to seal the broken DNA. Cellular DNA is constantly assaulted by ionizing radiation and reactive oxygen species. This damage, along with the products of some DNA repair enzymes, may contain 5' hydroxyls or 3' phosphates. These are converted by PNK to 5' phosphates and 3' hydroxyls, which are required for DNA polymerases and DNA ligases to complete repair of the damaged DNA. Productive engagement of a 3'-phosphate terminus may block access of a 5'-hydroxyl to the kinase active site
physiological function
-
bifunctional polynucleotide kinase/phosphatase contains both DNA 5'-kinase and 3'-phosphatase activities required for restoration of 3'-hydroxyls and 5'-phosphates needed to seal the broken DNA. Cellular DNA is constantly assaulted by ionizing radiation and reactive oxygen species. This damage, along with the products of some DNA repair enzymes, may contain 5' hydroxyls or 3' phosphates. These are converted by PNK to 5' phosphates and 3' hydroxyls, which are required for DNA polymerases and DNA ligases to complete repair of the damaged DNA. Productive engagement of a 3'-phosphate terminus may block access of a 5'-hydroxyl to the kinase active site
physiological function
Tequatrovirus T4
-
degradation of RegB-cleaved mRNAs depends on a functional T4 polynucleotide kinase/phosphatase. PNK controls the decay of early transcripts predominantly from their 5'-termini. The 5'-OH produced by RegB cleavage is phosphorylated by the kinase activity of PNK. When the 5'-OH RNA end generated by RegB is not phosphorylated by PNK, the attack by RNases E and G is blocked or decreased over a distance of about 300 nt from the RegB site. But after PNK has modified the 5'-terminus and RNase G (or E) has cut at secondary sites, the new 5'-monophosphorylated RNA ends can presumably activate RNases E and G in cascade. The PNK-dependent pathway of degradation becomes effective 5 min postinfectio. The T4 PNK also has a role during normal phage development
physiological function
-
polynucleotide kinase and aprataxin-like forkhead-associated protein (PALF) acts as both a single-stranded DNA endonuclease and a single-stranded DNA 3 exonuclease and can participate in DNA end joining in a biochemical system, they use the forkhead-associated domain to bind to x-ray repair complementing defective repair in Chinese hamster cells 4, XRCC4, overview
physiological function
-
polynucleotide kinase/phosphatase is an essential enzyme for the repair of damaged DNA termini. PNKP possesses both 5'-kinase and 3'-phosphatase activities that are frequently required for processing of single- and double-strand break termini
physiological function
-
polynucleotide kinase/phosphatase is an essential enzyme for the repair of damaged DNA termini. PNKP possesses both 5'-kinase and 3'-phosphatase activities that are frequently required for processing of single- and double-strand break termini
physiological function
-
polynucleotide kinase/phosphatase is an essential enzyme for the repair of damaged DNA termini. PNKP possesses both 5'-kinase and 3'-phosphatase activities that are frequently required for processing of single- and double-strand break termini
physiological function
Tequatrovirus T4
-
polynucleotide kinase/phosphatase is an essential enzyme for the repair of damaged DNA termini. PNKP possesses both 5'-kinase and 3'-phosphatase activities that are frequently required for processing of single- and double-strand break termini
physiological function
-
the nonribosomal protein Nol9 is a polynucleotide 5'-kinase that sediments primarily with the pre-60S and pre-40S ribosomal particles in HeLa nuclear extracts. The polynucleotide kinase activity of Nol9 is required for processing of large subunit rRNA and efficient generation of the 5.8S and 28S rRNAs from the 32S precursor
physiological function
Tequatrovirus T4
-
posphorylation and dephosphorylation of DNA by polynucleotide kinase has an important role in DNA damage repair, replication, and recombination
physiological function
mice with PNKP inactivation in neural progenitors manifest neurodevelopmental abnormalities and postnatal death. The phenotype involves defective base excision repair and nonhomologous end-joining. Mice homozygous for the T424GfsX48 frame-shift allele are lethal embryonically, and attenuated PNKP levels akin to microcephaly with seizures syndrome show general neurodevelopmental defects. Directed postnatal neural inactivation of PNKP affects specific subpopulations including oligodendrocytes
physiological function
-
PNKP stably associates with ataxin-3. Purified wild-type ataxin-3 stimulates, and the mutant form specifically inhibits, PNKP's 3'-phosphatase activity in vitro. ATXN3-deficient cells also show decreased PNKP activity
physiological function
-
polynucleotide kinase phosphatase is a DNA repair factor with dual enzymatic functions, i.e., phosphorylation of 5'-end and dephosphorylation of 3'-end, which are prerequisites for DNA ligation and, thus, is involved in multiple DNA repair pathways, i.e., base excision repair, single-strand break repair and double-strand break repair through nonhomologous end joining
additional information
-
molecular architecture of the enzyme, overview. The mammalian enzyme preferentially phosphorylates 5'-hydroxyl termini within nicked, gapped or double-strand breaks with single-stranded 3'-overhanging ends, whereas single-stranded 5'-termini or blunt double-stranded ends are phosphorylated less efficiently. The selective recognition of the larger, double-stranded DNA substrates is effected by a broad DNA recognition groove composed of two distinct positively charged surfaces. Mechanisms of single-strand break and double-strand break repairs, and of base excision repair, overview
additional information
-
molecular architecture of the enzyme, overview. The mammalian enzyme preferentially phosphorylates 5'-hydroxyl termini within nicked, gapped or DSBs with single-stranded 3' overhanging ends, whereas single-stranded 5'-termini or blunt double-stranded ends are phosphorylated less efficiently. The selective recognition of the larger, double-stranded DNA substrates is effected by a broad DNA recognition groove composed of two distinct positively charged surfaces. Mechanisms of single-strand break and double-strand break repairs, and base excision repair, overview
additional information
Tequatrovirus T4
-
molecular architecture of the enzyme, overview. The phage PNK DNA binding cleft forms a narrow channel leading to the conserved catalytic aspartic acid residue that accommodates single-stranded, but not double-stranded, substrates. Mechanisms of single-strand break and double-strand break repairs, overview
additional information
-
PNK domain architecture, overview
additional information
-
PNK domain architecture, overview
additional information
structure and mechanism of the polynucleotide kinase component of the bacterial Pnkp-Hen1 RNA repair system, the enzyme has an autonomous kinase domain, overview. Pnkp-Hen1 RNA repair pathway confers protective immunity to recurrent RNA damage
additional information
-
structure and mechanism of the polynucleotide kinase component of the bacterial Pnkp-Hen1 RNA repair system, the enzyme has an autonomous kinase domain, overview. Pnkp-Hen1 RNA repair pathway confers protective immunity to recurrent RNA damage
additional information
-
the nuclease activity of polynucleotide kinase and aprataxin-like forkhead-associated is not affected by Ku or XRCC4-DNA ligase IV
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D38E
site-directed mutagenesis, the mutant shows 6% of wild-type activity
D38N
site-directed mutagenesis, inactive mutant
H189D
-
mutation transforms enzyme into a Mn2+-dependent phosphodiesterase devoid of monoesterase activity. Phosphodiesterase activity of mutant is strictly specific for 2',3'-cyclic phosphates which it hydrolyzes to yield a 2'-NMP as the sole product
K21Q
site-directed mutagenesis, the mutant shows 100fold reduced activity compared to the wild-type enzyme
K21R
site-directed mutagenesis, the mutant shows 15fold reduced activity compared to the wild-type enzyme
K95A
site-directed mutagenesis, inactive mutant, the phenotype is benign
Q51A
the mutation reduces kinase specific activity about 3fold
R116A
the mutation elicits a 10fold increase in Km for ATP, but has little effect on the turnover number value leading to 83% of wild type activity
R41K
site-directed mutagenesis, the mutant shows about 65% reduced activity compared to the wild-type enzyme
R41Q
site-directed mutagenesis, the mutant shows 100fold reduced activity compared to the wild-type enzyme
S22T
site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type enzyme
S37A/T80A
the double mutation reduces kinase activity 50fold
T54A
the mutation reduces kinase specific activity about 3fold
V129A
the mutation reduces kinase specific activity about 3fold
Q51A
-
the mutation reduces kinase specific activity about 3fold
-
S37A/T80A
-
the double mutation reduces kinase activity 50fold
-
T54A
-
the mutation reduces kinase specific activity about 3fold
-
V129A
-
the mutation reduces kinase specific activity about 3fold
-
D171A
-
complete loss of activity
D173A
-
complete loss of activity
K138A/R35A
-
the mutant shows impaired nuclear localization as compared to the wild type enzyme
K378A
-
complete loss of activity
W331F
single mutant, 90% phosphatase activity
W402F
single mutant, 85% phosphatase activity
WFX402
mutant, all tryptophans except 402 are replaced by phenylalanine, 85% phosphatase activity
D169A
Kostyavirus CJW1
-
50% of wild-type activity with 37-mer oligodeoxyribonucleotide substrate, no reverse reaction
K15A
Kostyavirus CJW1
-
no enzymic activity in kinase reaction, 58% of wild-type activity in 3-phosphates reaction
D175A
Omegavirus omega
-
60% of wild-type activity with 37-mer oligodeoxyribonucleotide substrate, 68% of wild-type activity with CMP substrate
K16A
Omegavirus omega
-
no enzymic activity
D73A
mutation abolishes activity
K49A
mutation reduces activity to 1% of the wild-type value
H73A
-
the mutant shows wild type activity
H73A
-
the mutant shows wild type activity
-
D165A
Tequatrovirus T4
-
site-directed mutagenesis, increased activity compared to the wild-type, no 3'-phosphatase activity remaining
D180A
Tequatrovirus T4
-
site-directed mutagenesis, slightly higher activity than the wild-type, reduced 3'-phosphatase activity
D250A
Tequatrovirus T4
-
site-directed mutagenesis, increased activity compared to the wild-type , reduced 3'-phosphatase activity
D254A
Tequatrovirus T4
-
site-directed mutagenesis, increased activity compared to the wild-type, nearly no 3'-phosphatase activity remaining
D278A
Tequatrovirus T4
-
site-directed mutagenesis, increased activity compared to the wild-type, no 3'-phosphatase activity remaining
G14D
Tequatrovirus T4
-
the mutation impairs the 5`-kinase activity of the enzyme in vivo as well as in vitro and leads to diminished processing at secondary sites of several RegB-cleaved transcripts
K125A
Tequatrovirus T4
-
site-directed mutagenesis, slightly higher activity than the wild-type
R122A
Tequatrovirus T4
-
site-directed mutagenesis, slightly higher activity than the wild-type, reduced 3'-phosphatase activity
R126A
Tequatrovirus T4
-
site-directed mutagenesis, highly reduced activity
R176A
Tequatrovirus T4
-
site-directed mutagenesis, increased activity compared to the wild-type, highly reduced 3'-phosphatase activity
R213A
Tequatrovirus T4
-
site-directed mutagenesis, increased activity compared to the wild-type, no 3'-phosphatase activity remaining
R229H
Tequatrovirus T4
-
the mutant shows slightly reduced wild type activity
R246A
Tequatrovirus T4
-
site-directed mutagenesis, increased activity compared to the wild-type, highly reduced 3'-phosphatase activity
R279A
Tequatrovirus T4
-
site-directed mutagenesis, increased activity compared to the wild-type, highly reduced 3'-phosphatase activity
R36A
Tequatrovirus T4
-
site-directed mutagenesis, similar activity than the wild-type
R38A
Tequatrovirus T4
-
site-directed mutagenesis, highly reduced activity, reduced 3'-phosphatase activity
S84A
Tequatrovirus T4
-
site-directed mutagenesis, slightly lower activity than the wild-type, reduced 3'-phosphatase activity
G14D
Tequatrovirus T4 K10
-
the mutation impairs the 5`-kinase activity of the enzyme in vivo as well as in vitro and leads to diminished processing at secondary sites of several RegB-cleaved transcripts
-
R229H
Tequatrovirus T4 K10
-
the mutant shows slightly reduced wild type activity
-
R140H
the mutation does not destabilize the protein but substantially impairs kinase activity (less than 40% compared to the wild type). The mutation is associated with degeneration/hypoplasia of the central nervous system
R140H
-
the mutation negatively affects enzyme function
additional information
Tequatrovirus T4
-
natural mutants pseT 1 and pseT 47, the first shows no 3'-phosphatase activity, but normal polynucleotide kinase activity, the second shows very little 3'-phosphatase activity, but no polynucleotide kinase activity, expression in Escherichia coli
additional information
-
expression of wild-type C-terminal kinase domain, amino acids 394-694 with His10-tag gives a 38 kDa protein with enzymic activity. Mutant K407A of the domain has no enzymic activity. Mutation D560A has no effect
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Habraken, Y.; Verly, W.G.
Further purification and characterization of the DNA 3-phosphatase from rat-liver chromatin which is also a polynucleotide 5-hydroxyl kinase
Eur. J. Biochem.
171
59-66
1988
Rattus norvegicus
brenda
Habraken, Y.; Verly, W.G.
The DNA 3-phosphatase and 5-hydroxyl kinase of rat liver chromatin
FEBS Lett.
160
46-50
1983
Rattus norvegicus
brenda
Maunders, M.J.
Polynucleotide kinase (EC 2.7.1.78)
Methods Mol. Biol.
16
343-357
1993
Escherichia phage T2, Tequatrovirus T4, Enterobacteria phage T6, Bos taurus, Homo sapiens, Mesocricetus auratus, no activity in Escherichia coli, Rattus norvegicus
brenda
Novogrodsky, A.; Hurwitz, J.
The enzymatic phosphorylation of ribonucleic acid and deoxyribonucleic acid. I. Phosphorylation at 5-hydroxyl termini
J. Biol. Chem.
241
2923-2932
1966
Escherichia phage T2, Tequatrovirus T4, no activity in bacteriophage T1, no activity in bacteriophage T5
brenda
Novogrodsky, A.; Tal, M.; Traub, A.; Hurwitz, J.
The enzymatic phosphorylation of ribonucleic acid and deoxyribonucleic acid. II. Further properties of the 5-hydroxyl polynucleotide kinase
J. Biol. Chem.
241
2933-2943
1966
Escherichia phage T2
brenda
Fejes, E.; Denes, G.
Purification and some properties of polynucleotide kinase from rat liver nuclei
Acta Biochim. Biophys. Acad. Sci. Hung.
15
275-285
1980
Rattus norvegicus
brenda
Panet, A.; van de Sande, J.H.; Loewen, P.C.; Khorana, H.G.; Raae, A.J.; Lillehaug, J.R.; Kleppe, K.
Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase
Biochemistry
12
5045-5050
1973
Tequatrovirus T4
brenda
Lillehaug, J.R.; Kleppe, R.K.; Kleppe, K.
Phosphorylation of double-stranded DNAs by T4 polynucleotide kinase
Biochemistry
15
1858-1865
1976
Tequatrovirus T4
brenda
Teraoka, H.; Mizuta, K.; Sato, F.; Shimoyachi, M.; Tsukada, K.
Polynucleotide kinase from rat-liver nuclei. Purification and properties
Eur. J. Biochem.
58
297-302
1975
Rattus norvegicus
brenda
Lillehaug, J.R.
Physicochemical properties of T4 polynucleotide kinase
Eur. J. Biochem.
73
499-506
1977
Tequatrovirus T4
brenda
Tamura, S.; Teraoka, H.; Tsukada, K.
Characterization of DNA kinase from calf thymus
Eur. J. Biochem.
115
449-453
1981
Bos taurus
brenda
Nichols, B.P.; Lindell, T.D.; Stellwagen, E.; Donelson, J.E.
A rapid purification of T4 polynucleotide kinase using Blue Dextran-Sepharose chromatography
Biochim. Biophys. Acta
526
410-417
1978
Tequatrovirus T4
brenda
Lillehaug, J.R.
Inhibition of T4 polynucleotide kinase by the ATP analog, beta, gamma-imidoadenylyl 5-triphosphate
Biochim. Biophys. Acta
525
357-363
1978
Tequatrovirus T4
brenda
Pheiffer, B.H.; Zimmerman, S.B.
Deoxyribonucleic acid kinase from nuclei of rat liver: mechanism, reversal, and inhibitors of the reaction
Biochemistry
18
2960-2963
1979
Rattus norvegicus
brenda
Sertic-Pritsos, K.; Vinocour, M.; Winicov, I.
5-Hydroxyl RNA kinase from mouse L cells
Eur. J. Biochem.
144
47-55
1984
Mus musculus
brenda
Soltis, D.A.; Uhlenbeck, O.C.
Isolation and characterization of two mutant forms of T4 polynucleotide kinase
J. Biol. Chem.
257
11332-11339
1982
Tequatrovirus T4
brenda
Bosdal, T.; Lillehaug, J.R.
Purification and kinetic properties of polynucleotide kinase from rat testes
Biochim. Biophys. Acta
840
280-286
1985
Rattus norvegicus
brenda
Shuman, S.; Hurwitz, J.
5-Hydroxyl polyribonucleotide kinase from HeLa cell nuclei. Purification and properties
J. Biol. Chem.
254
10396-10404
1979
Homo sapiens
brenda
Levin, C.J.; Zimmerman, S.B.
A deoxyribonucleic acid kinase from nuclei of rat liver. Purification and properties
J. Biol. Chem.
251
1767-1774
1976
Rattus norvegicus
brenda
Austin, G.E.; Sirakoff, D.; Roop, B.; Moyer, G.H.
Purification and properties of polynucleotide kinase of calf thymus
Biochim. Biophys. Acta
522
412-422
1978
Bos taurus
brenda
Lorsch, J.R.; Szostak, J.W.
Kinetic and thermodynamic characterization of the reaction catalyzed by a polynucleotide kinase ribozyme
Biochemistry
34
15315-15327
1995
synthetic ribozyme
brenda
Prinos, P.; Slack, C.; Lasko, D.D.
5' Phosphorylation of DNA in mammalian cells: identification of a polymin P-precipitable polynucleotide kinase
J. Cell. Biochem.
58
115-131
1995
Bos taurus
brenda
Karimi-Busheri, F.; Weinfeld, M.
Purification and substrate specificity of polydeoxyribonucleotide kinases isolated from calf thymus and rat liver
J. Cell. Biochem.
64
258-272
1997
Bos taurus, Rattus norvegicus
brenda
Fanta, M.; Zhang, H.; Bernstein, N.; Glover, M.; Karimi-Busheri, F.; Weinfeld, M.
Production, characterization, and epitope mapping of monoclonal antibodies against human polydeoxyribonucleotide kinase
Hybridoma
20
237-242
2001
Homo sapiens
brenda
Wang, L.K.; Shuman, S.
Mutational analysis defines the 5'-kinase and 3'-phosphatase active sites of T4 polynucleotide kinase
Nucleic Acids Res.
30
1073-1080
2002
Tequatrovirus T4
brenda
Petrousseva, I.O.; Safronov, I.V.; Komarova, N.I.; Kamynina, T.P.; Lavrik, O.I.; Khodyreva, S.N.
A new approach to the synthesis of the 5'-end substituted oligonucleotides using T4 polynucleotide kinase and gamma-amides of ATP bearing photoreactive groups
Dokl. Biochem. Biophys.
389
114-117
2003
Tequatrovirus T4
brenda
Caldecott, K.W.
Polynucleotide kinase. A versatile molecule makes a clean break
Structure
10
1151-1152
2002
Tequatrovirus T4, Mammalia
brenda
Zhu, H.; Yin, S.; Shuman, S.
Characterization of polynucleotide kinase/phosphatase enzymes from Mycobacteriophages omega and Cjw1 and vibriophage KVP40
J. Biol. Chem.
279
26358-26369
2004
Omegavirus omega, Kostyavirus CJW1
brenda
Mani, R.S.; Karimi-Busheri, F.; Fanta, M.; Cass, C.E.; Weinfeld, M.
Spectroscopic studies of DNA and ATP binding to human polynucleotide kinase: evidence for a ternary complex
Biochemistry
42
12077-12084
2003
Homo sapiens
brenda
Martins, A.; Shuman, S.
Characterization of a baculovirus enzyme with RNA ligase, polynucleotide 5'-Kinase, and polynucleotide 3'-phosphatase activities
J. Biol. Chem.
279
18220-18231
2004
unidentified baculovirus
brenda
Blondal, T.; Hjorleifsdottir, S.; Aevarsson, A.; Fridjonsson, O.H.; Skirnisdottir, S.; Wheat, J.O.; Hermannsdottir, A.G.; Hreggvidsson, G.O.; Smith, A.V.; Kristjansson, J.K.
Characterization of a 5'-polynucleotide kinase/3'-phosphatase from bacteriophage RM378
J. Biol. Chem.
280
5188-5194
2005
Rhodothermus phage RM378
brenda
Bernstein, N.K.; Williams, R.S.; Rakovszky, M.L.; Cui, D.; Green, R.; Karimi-Busheri, F.; Mani, R.S.; Galicia, S.; Koch, C.A.; Cass, C.E.; Durocher, D.; Weinfeld, M.; Glover, J.N.
The molecular architecture of the mammalian DNA repair enzyme, polynucleotide kinase
Mol. Cell
17
657-670
2005
Mus musculus (Q9JLV6)
brenda
Eastberg, J.H.; Pelletier, J.; Stoddard, B.L.
Recognition of DNA substrates by T4 bacteriophage polynucleotide kinase
Nucleic Acids Res.
32
653-660
2004
Tequatrovirus T4 (P06855)
brenda
Karimi-Busheri, F.; Rasouli-Nia, A.; Allalunis-Turner, J.; Weinfeld, M.
Human polynucleotide kinase participates in repair of DNA double-strand breaks by nonhomologous end joining but not homologous recombination
Cancer Res.
67
6619-6625
2007
Homo sapiens
brenda
Keppetipola, N.; Shuman, S.
Distinct enzymic functional groups are required for the phosphomonoesterase and phosphodiesterase activities of Clostridium thermocellum polynucleotide kinase/phosphatase
J. Biol. Chem.
281
19251-19259
2006
Acetivibrio thermocellus
brenda
Audebert, M.; Salles, B.; Weinfeld, M.; Calsou, P.
Involvement of polynucleotide kinase in a poly(ADP-ribose) polymerase-1-dependent DNA double-strand breaks rejoining pathway
J. Mol. Biol.
356
257-265
2006
Homo sapiens
brenda
Dobson, C.J.; Allinson, S.L.
The phosphatase activity of mammalian polynucleotide kinase takes precedence over its kinase activity in repair of single strand breaks
Nucleic Acids Res.
34
2230-2237
2006
Homo sapiens
brenda
Keppetipola, N.; Shuman, S.
Mechanism of the phosphatase component of Clostridium thermocellum polynucleotide kinase-phosphatase
RNA
12
73-82
2006
Acetivibrio thermocellus
brenda
Keppetipola, N.; Shuman, S.
Characterization of the 2,3 cyclic phosphodiesterase activities of Clostridium thermocellum polynucleotide kinase-phosphatase and bacteriophage lambda phosphatase
Nucleic Acids Res.
35
7721-7732
2007
Acetivibrio thermocellus
brenda
Song, C.; Zhao, M.
Real-time monitoring of the activity and kinetics of T4 polynucleotide kinase by a singly labeled DNA-hairpin smart probe coupled with lambda exonuclease cleavage
Anal. Chem.
81
1383-1388
2009
Tequatrovirus T4 (P06855)
brenda
Freschauf, G.K.; Karimi-Busheri, F.; Ulaczyk-Lesanko, A.; Mereniuk, T.R.; Ahrens, A.; Koshy, J.M.; Rasouli-Nia, A.; Pasarj, P.; Holmes, C.F.; Rininsland, F.; Hall, D.G.; Weinfeld, M.
Identification of a small molecule inhibitor of the human DNA repair enzyme polynucleotide kinase/phosphatase
Cancer Res.
69
7739-7746
2009
Tequatrovirus T4, Homo sapiens, Schizosaccharomyces pombe, Mus musculus (Q9JLV6)
brenda
Whiteside, J.R.; Box, C.L.; McMillan, T.J.; Allinson, S.L.
Cadmium and copper inhibit both DNA repair activities of polynucleotide kinase
DNA Repair
9
83-89
2010
Homo sapiens
brenda
Freschauf, G.K.; Mani, R.S.; Mereniuk, T.R.; Fanta, M.; Virgen, C.A.; Dianov, G.L.; Grassot, J.M.; Hall, D.G.; Weinfeld, M.
Mechanism of action of an imido-piperidine inhibitor of human polynucleotide kinase/phosphatase
J. Biol. Chem.
285
2351-2360
2010
Homo sapiens (Q96T60)
brenda
Jain, R.; Shuman, S.
Characterization of a thermostable archaeal polynucleotide kinase homologous to human Clp1
RNA
15
923-931
2009
Pyrococcus horikoshii (Q57936), Pyrococcus horikoshii
brenda
Weinfeld, M.; Mani, R.S.; Abdou, I.; Aceytuno, R.D.; Glover, J.N.
Tidying up loose ends: the role of polynucleotide kinase/phosphatase in DNA strand break repair
Trends Biochem. Sci.
36
262-271
2011
Tequatrovirus T4, Homo sapiens, Mus musculus, Schizosaccharomyces pombe
brenda
Heindl, K.; Martinez, J.
Nol9 is a novel polynucleotide 5-kinase involved in ribosomal RNA processing
EMBO J.
29
4161-4171
2010
Homo sapiens
brenda
Li, S.; Kanno, S.; Watanabe, R.; Ogiwara, H.; Kohno, T.; Watanabe, G.; Yasui, A.; Lieber, M.R.
Polynucleotide kinase and aprataxin-like forkhead-associated protein (PALF) acts as both a single-stranded DNA endonuclease and a single-stranded DNA 3 exonuclease and can participate in DNA end joining in a biochemical system
J. Biol. Chem.
286
36368-36377
2011
Homo sapiens
brenda
Schellenberg, M.; Williams, R.
DNA end processing by polynucleotide kinase/phosphatase
Proc. Natl. Acad. Sci. USA
108
20855-20856
2011
Homo sapiens, Mus musculus
brenda
Durand, S.; Richard, G.; Bontems, F.; Uzan, M.
Bacteriophage T4 polynucleotide kinase triggers degradation of mRNAs
Proc. Natl. Acad. Sci. USA
109
7073-7078
2012
Tequatrovirus T4
brenda
Wang, L.K.; Das, U.; Smith, P.; Shuman, S.
Structure and mechanism of the polynucleotide kinase component of the bacterial Pnkp-Hen1 RNA repair system
RNA
18
2277-2286
2012
Acetivibrio thermocellus (A3DJ38), Acetivibrio thermocellus
brenda
Mair, B.; Popow, J.; Mechtler, K.; Weitzer, S.; Martinez, J.
Intron excision from precursor tRNA molecules in mammalian cells requires ATP hydrolysis and phosphorylation of tRNA-splicing endonuclease components
Biochem. Soc. Trans.
41
831-837
2013
Homo sapiens
brenda
Karaca, E.; Weitzer, S.; Pehlivan, D.; Shiraishi, H.; Gogakos, T.; Hanada, T.; Jhangiani, S.N.; Wiszniewski, W.; Withers, M.; Campbell, I.M.; Erdin, S.; Isikay, S.; Franco, L.M.; Gonzaga-Jauregui, C.; Gambin, T.; Gelowani, V.; Hunter, J.V.; Yesil, G.; Koparir, E.; Yilmaz, S.; Brown, M.; Briskin, D.; H, H.a.
Human CLP1 mutations alter tRNA biogenesis, affecting both peripheral and central nervous system function
Cell
157
636-650
2014
Homo sapiens
brenda
Schaffer, A.E.; Eggens, V.R.; Caglayan, A.O.; Reuter, M.S.; Scott, E.; Coufal, N.G.; Silhavy, J.L.; Xue, Y.; Kayserili, H.; Yasuno, K.; Rosti, R.O.; Abdellateef, M.; Caglar, C.; Kasher, P.R.; Cazemier, J.L.; Weterman, M.A.; Cantagrel, V.; Cai, N.; Zweier, C.; Altunoglu, U.; Satkin, N.B.; Aktar, F.; Tuysuz, B.
CLP1 founder mutation links tRNA splicing and maturation to cerebellar development and neurodegeneration
Cell
157
651-663
2014
Homo sapiens (Q92989), Homo sapiens
brenda
Das, U.; Shuman, S.
Mechanism of RNA 2,3-cyclic phosphate end healing by T4 polynucleotide kinase-phosphatase
Nucleic Acids Res.
41
355-365
2013
Tequatrovirus T4
brenda
Ma, C.; Fang, H.; Wang, K.; Xia, K.; Chen, H.; He, H.; Zeng, W.
Simultaneous detection of kinase and phosphatase activities of polynucleotide kinase using molecular beacon probes
Anal. Biochem.
443
166-168
2013
Tequatrovirus T4
brenda
Tao, M.; Shi, Z.; Cheng, R.; Zhang, J.; Li, B.; Jin, Y.
Highly specific fluorescence detection of T4 polynucleotide kinase activity via photo-induced electron transfer
Anal. Biochem.
485
18-24
2015
Tequatrovirus T4
brenda
Hou, T.; Wang, X.; Liu, X.; Lu, T.; Liu, S.; Li, F.
Amplified detection of T4 polynucleotide kinase activity by the coupled lambda exonuclease cleavage reaction and catalytic assembly of bimolecular beacons
Anal. Chem.
86
884-890
2014
Tequatrovirus T4
brenda
Wang, G.; Chen, L.; He, X.; Zhu, Y.; Zhang, X.
Detection of polynucleotide kinase activity by using a gold electrode modified with magnetic microspheres coated with titanium dioxide nanoparticles and a DNA dendrimer
Analyst
139
3895-3900
2014
Tequatrovirus T4
brenda
Zhou, F.; Wang, G.; Shi, D.; Sun, Y.; Sha, L.; Qiu, Y.; Zhang, X.
One-strand oligonucleotide probe for fluorescent label-free "turn-on" detection of T4 polynucleotide kinase activity and its inhibition
Analyst
140
5650-5655
2015
Tequatrovirus T4
brenda
Strazdaite-Zieliene, Z.; Zajanckauskaite, A.; Kaliniene, L.; Meskys, R.; Truncaite, L.
A mutation in the gene for polynucleotide kinase of bacteriophage T4 K10 affects mRNA processing
Arch. Virol.
159
327-331
2014
Tequatrovirus T4, Tequatrovirus T4 K10
brenda
Das, U.; Wang, L.K.; Smith, P.; Shuman, S.
Structural and biochemical analysis of the phosphate donor specificity of the polynucleotide kinase component of the bacterial pnkp-hen1 RNA repair system
Biochemistry
52
4734-4743
2013
Acetivibrio thermocellus (A3DJ38), Acetivibrio thermocellus
brenda
Zhang, L.; Zhao, J.; Zhang, H.; Jiang, J.; Yu, R.
Double strand DNA-templated copper nanoparticle as a novel fluorescence indicator for label-free detection of polynucleotide kinase activity
Biosens. Bioelectron.
44
6-9
2013
Tequatrovirus T4
brenda
Chen, F.; Zhao, Y.; Qi, L.; Fan, C.
One-step highly sensitive florescence detection of T4 polynucleotide kinase activity and biological small molecules by ligation-nicking coupled reaction-mediated signal amplification
Biosens. Bioelectron.
47
218-224
2013
Tequatrovirus T4
brenda
Lian, S.; Liu, C.; Zhang, X.; Wang, H.; Li, Z.
Detection of T4 polynucleotide kinase activity based on cationic conjugated polymer-mediated fluorescence resonance energy transfer
Biosens. Bioelectron.
66
316-320
2015
Tequatrovirus T4
brenda
Das, U.; Wang, L.K.; Smith, P.; Munir, A.; Shuman, S.
Structures of bacterial polynucleotide kinase in a Michaelis complex with nucleoside triphosphate (NTP)-Mg2+ and 5-OH RNA and a mixed substrate-product complex with NTP-Mg2+ and a 5-phosphorylated oligonucleotide
J. Bacteriol.
196
4285-4292
2014
Acetivibrio thermocellus (A3DJ38), Acetivibrio thermocellus, Acetivibrio thermocellus DSM 1237 (A3DJ38)
brenda
Dikfidan, A.; Loll, B.; Zeymer, C.; Magler, I.; Clausen, T.; Meinhart, A.
RNA specificity and regulation of catalysis in the eukaryotic polynucleotide kinase Clp1
Mol. Cell
54
975-986
2014
Caenorhabditis elegans (P52874), Caenorhabditis elegans
brenda
Castle, C.; Sardana, R.; Dandekar, V.; Borgianini, V.; Johnson, A.; Denicourt, C.
Las1 interacts with Grc3 polynucleotide kinase and is required for ribosome synthesis in Saccharomyces cerevisiae
Nucleic Acids Res.
41
1135-1150
2013
Saccharomyces cerevisiae
brenda
Ma, C.; Jin, S.; Wang, J.; Wang, K.; Liu, H.; Wu, K.
A fluorescence-based assay for T4 polynucleotide kinase/phosphatase activity based on a terminal transferase-aided photoinduced electron transfer strategy
Anal. Methods
8
1989-1994
2016
Tequatrovirus T4 (P06855)
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brenda
Shimada, M.; Dumitrache, L.C.; Russell, H.R.; McKinnon, P.J.
Polynucleotide kinase-phosphatase enables neurogenesis via multiple DNA repair pathways to maintain genome stability
EMBO J.
34
2465-2480
2015
Mus musculus (Q9JLV6)
brenda
Zhang, X.; Liu, Q.; Jin, Y.; Li, B.
Determination of the activity of T4 polynucleotide kinase phosphatase by exploiting the sequence-dependent fluorescence of DNA-templated copper nanoclusters
Microchim. Acta
186
003
2019
Tequatrovirus T4 (P06855)
brenda
Chatterjee, A.; Saha, S.; Chakraborty, A.; Silva-Fernandes, A.; Mandal, S.M.; Neves-Carvalho, A.; Liu, Y.; Pandita, R.K.; Hegde, M.L.; Hegde, P.M.; Boldogh, I.; Ashizawa, T.; Koeppen, A.H.; Pandita, T.K.; Maciel, P.; Sarkar, P.S.; Hazra, T.K.
The role of the mammalian DNA end-processing enzyme polynucleotide kinase 3-phosphatase in spinocerebellar ataxia type 3 pathogenesis
PLoS Genet.
11
e1004749
2015
Homo sapiens
brenda
Dong, Z.; Zhang, L.; Qiao, M.; Ge, J.; Liu, A.; Li, Z.
A label-free assay for T4 polynucleotide kinase/phosphatase activity and its inhibitors based on poly(thymine)-templated copper nanoparticles
Talanta
146
253-258
2015
Tequatrovirus T4 (P06855)
brenda
Zhang, H.; Zhao, Z.; Lei, Z.; Wang, Z.
Sensitive detection of polynucleotide kinase activity by paper-based fluorescence assay with lambda exonuclease assistance
Anal. Chem.
88
11358-11363
2016
Tequatrovirus T4
brenda
Feng, C.; Wang, Z.; Chen, T.; Chen, X.; Mao, D.; Zhao, J.; Li, G.
A dual-enzyme-assisted three-dimensional DNA walking machine using T4 polynucleotide kinase as activators and application in polynucleotide kinase assays
Anal. Chem.
90
2810-2815
2018
Tequatrovirus T4
brenda
Munir, A.; Shuman, S.
Characterization of Runella slithyformis HD-Pnk, a bifunctional DNA/RNA end-healing enzyme composed of an N-terminal 2,3-phosphoesterase HD domain and a C-terminal 5'-OH polynucleotide kinase domain
J. Bacteriol.
199
e00739-16
2017
Runella slithyformis, Runella slithyformis ATCC 49304
brenda
Tsukada, K.; Matsumoto, Y.; Shimada, M.
Linker region is required for efficient nuclear localization of polynucleotide kinase phosphatase
PLoS ONE
15
e0239404
2020
Homo sapiens
brenda