Information on EC 2.7.1.85 - beta-glucoside kinase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.85
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RECOMMENDED NAME
GeneOntology No.
beta-glucoside kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + cellobiose = ADP + 6-phospho-beta-D-glucosyl-(1->4)-D-glucose
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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SYSTEMATIC NAME
IUBMB Comments
ATP:cellobiose 6-phosphotransferase
Phosphorylates a number of beta-D-glucosides; GTP, CTP, ITP and UTP can also act as donors.
CAS REGISTRY NUMBER
COMMENTARY hide
37205-53-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ATCC 23357
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Manually annotated by BRENDA team
CLIP 11262
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Manually annotated by BRENDA team
Listeria innocua CLIP 11262
CLIP 11262
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Manually annotated by BRENDA team
EGD-e
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + amygdalin
ADP + amygdalin 6-phosphate
show the reaction diagram
ATP + arbutin
ADP + arbutin 6-phosphate
show the reaction diagram
ATP + cellobiitol
?
show the reaction diagram
ATP + cellobiose
ADP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
show the reaction diagram
ATP + cellotetraose
ADP + 6-phospho-beta-glucosyl-(1,4)-beta-D-glucosyl-(1,4)-beta-D-glucosyl-(1,4)-D-glucose
show the reaction diagram
ATP + cellotriose
ADP + 6-phospho-beta-D-glucosyl-(1,4)-beta-D-glucosyl-(1,4)-D-glucose
show the reaction diagram
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47% of the activity with cellobiose
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-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + gentiobiose
ADP + 6-phospho-beta-D-glucosyl-(1,6)-D-glucose
show the reaction diagram
ATP + isopropyl beta-D-thioglucopyranoside
?
show the reaction diagram
-
-
-
-
?
ATP + isopropyl beta-D-thioglucopyranoside
ADP + isopropyl beta-D-thioglucopyranoside 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + laminaribiose
ADP + 6-phospho-beta-D-glucopyranosyl-(1,3)-D-glucose
show the reaction diagram
-
-
-
-
?
ATP + methyl beta-D-glucoside
ADP + methyl beta-D-glucoside 6-phosphate
show the reaction diagram
ATP + n-octyl-beta-D-glucopyranoside
ADP + n-octyl-beta-D-glucopyranoside 6-phosphate
show the reaction diagram
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-
-
-
?
ATP + phenyl beta-D-glucoside
ADP + phenyl beta-D-glucoside 6-phosphate
show the reaction diagram
ATP + salicin
ADP + salicin 6-phosphate
show the reaction diagram
ATP + sophorose
ADP + 6-phospho-beta-D-glucopyranosyl-(1,2)-D-glucose
show the reaction diagram
ATP + thiocellobiose
?
show the reaction diagram
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-
-
-
?
CTP + cellobiose
CDP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
show the reaction diagram
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12% of the activity with ATP
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?
GTP + cellobiose
GDP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
show the reaction diagram
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15% of the activity with ATP
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?
ITP + cellobiose
IDP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
show the reaction diagram
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7% of the activity with ATP
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?
UTP + cellobiose
UDP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
show the reaction diagram
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3% of the activity with ATP
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?
additional information
?
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not: ADP, acetyl phosphate, alpha-glycerophosphate, D-fructose, 1,6-diphosphate, phosphoramidate, choline phosphate, creatine phosphate, 3-phosphoglycerate, phenylphosphate, phosphoenolpyruvate
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + cellobiose
ADP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
show the reaction diagram
ATP + gentiobiose
ADP + 6-phospho-beta-D-glucosyl-(1,6)-D-glucose
show the reaction diagram
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inducible enzyme that functions in metabolism of cellobiose and gentiobiose
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-
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NEM
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considerable protection
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.47 - 3.1
amygdalin
0.21 - 0.6
Arbutin
0.24 - 1.7
ATP
1.56 - 4
cellobiitol
0.47 - 1
cellobiose
6.3
Cellotetraose
-
pH 7.5
6.7
cellotriose
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pH 7.5
40.29
D-glucose
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pH 7.5
0.56 - 1.5
gentiobiose
2.03
isopropyl beta-D-thioglucopyranoside
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pH 7.5
0.63
laminaribiose
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pH 7.5
2.23
methyl beta-D-glucopyranoside
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pH 7.5
13.1
methyl beta-D-glucoside
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pH 7.5
0.11
n-octyl beta-D-glucopyranoside
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pH 7.5
0.65
phenyl beta-D-glucopyranoside
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pH 7.5
2.5
phenyl beta-D-glucoside
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pH 7.5
0.18 - 2.5
Salicin
3.1 - 3.15
sophorose
10.54
thiocellobiose
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pH 7.5
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2
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glycylglycine buffer
7.3
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MES buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2 - 8.2
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pH 6.2: about 60% of maximal activity in Mes buffer, pH 8.2: reaction in PIPES buffer, about 70% of maximal activity in MES buffer
PDB
SCOP
CATH
ORGANISM
UNIPROT
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
150000
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sucrose density gradient centrifugation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
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4 * 32697, calculation from nucleotide sequence; 4 * 33000, non-covalently linked subunits, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
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half-life: 10 min
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 35% loss of activity after 2 months
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frozen, 50% loss of activity after 2 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloning and high expression of His6BglK in Escherichia coli TOP10
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D103G
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catalytically inactive protein
D7G
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catalytically inactive protein
G131A
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catalytically inactive protein
G133A
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catalytically inactive protein
G9A
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catalytically inactive protein
Show AA Sequence (322 entries)
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