Information on EC 2.7.2.15 - propionate kinase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.2.15
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RECOMMENDED NAME
GeneOntology No.
propionate kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + acetate = ADP + acetyl phosphate
show the reaction diagram
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-
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ATP + propanoate = ADP + propanoyl phosphate
show the reaction diagram
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-1,2-propanediol degradation
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L-threonine degradation I
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threonine metabolism
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Propanoate metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
ATP:propanoate phosphotransferase
Requires Mg2+. Acetate can also act as a substrate. Involved in the anaerobic degradation of L-threonine in bacteria [1]. Both this enzyme and EC 2.7.2.1, acetate kinase, play important roles in the production of propanoate [1].
CAS REGISTRY NUMBER
COMMENTARY hide
39369-28-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetate + ATP
acetyl phosphate + ADP
show the reaction diagram
propionate + ATP
propionyl phosphate + ADP
show the reaction diagram
additional information
?
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TdcD has Ap4A synthetic activity in the presence of ATP
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
98.2
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acetate, 25C, after overproduction of TdcD
114.7
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propionate, 25C, after overproduction of TdcD
additional information
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cell extracts enriched for the PduW or TdcD protein show levels of propionate kinase activity that were about 11 and 45 fold higher than the background
PDB
SCOP
CATH
ORGANISM
UNIPROT
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43340
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calculated
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by the hanging drop method, crystals of native TdcD (2.6 A resolution), TdcD-Ap4A complex in the presence of ATP (2.0 A resolution), and TdcD-Ap4A (2.4 A resolution), Ap4A is bound at the active site pocket, Ap4A is present in an extended conformation with one adenosine moiety present in the nucleotide binding site and other in the propionate binding site
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity by Ni-NTA affinity column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ackA gene is expressed in Escherichia coli to efficiently convert propionyl-phosphate to propionate
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cloned in pRSET C vector and overexpressed in Escherichia coli BL21(DE3)pLysS along with an N-terminal hexahistidine tag
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
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in order to realise an efficient production of 1-propanol through amino acid biosynthetic pathway using glucose or glycerol as a carbon source L-threonine overproducing strain, TH20 is employed as a base strain and engineered to establish a novel pathway leading to the formation of1-propanol under aerobic condition. The engineered strain, PRO2, harbouring a plasmid overexpressing the atoDA, adhEmut, thrABC, ackA and cimA genes is able to produce more than 10 g/L of 1-propanol from glucose or glycerol in aerobic fed-batch fermentation
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