Information on EC 2.7.7.10 - UTP-hexose-1-phosphate uridylyltransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.7.7.10
-
RECOMMENDED NAME
GeneOntology No.
UTP-hexose-1-phosphate uridylyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UTP + alpha-D-galactose 1-phosphate = diphosphate + UDP-alpha-D-galactose
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-galactose degradation III
-
-
stachyose degradation
-
-
degradation of hexoses
-
-
Galactose metabolism
-
-
Amino sugar and nucleotide sugar metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
UTP:alpha-D-hexose-1-phosphate uridylyltransferase
alpha-D-Glucose 1-phosphate can also act as acceptor, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
9016-11-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
glucose-grown
-
-
Manually annotated by BRENDA team
cv. Burpee's Hybrid
-
-
Manually annotated by BRENDA team
cv. Calypiso
-
-
Manually annotated by BRENDA team
strain K-9, ATCC 30015
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Balb/c mice
-
-
Manually annotated by BRENDA team
Saccharomyces fragilis
galactose-adopted
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
complete, or near complete, deficiency of GALT causes classic galactosaemia
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
diphosphate + UDP-xylose
?
show the reaction diagram
-
at 10% the rate of UDP-glucose or UDP-galactose
-
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-alpha-D-galactose
show the reaction diagram
-
-
-
-
?
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-galactose
show the reaction diagram
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-alpha-D-galactose
show the reaction diagram
-
-
-
-
?
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-galactose
show the reaction diagram
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
activation
additional information
-
phosphate is no effector of UDP-galactose synthesis
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Mg-diphosphate
-
strong inhibition, UDP-galactose synthesis
UDP-galactose
UDP-glucose
-
UDP-galactose pyrophosphorolysis
UDP-xylose
-
UDP-galactose or UDP-glucose synthesis
UTP
-
UDP-galactose pyrophosphorolysis
additional information
-
no inhibitors of UDP-galactose pyrophosphorolysis are alpha-D-glucose 1-phosphate, alpha-D-galactose 1-phosphate or galactose 6-phosphate
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-phosphoglycerate
-
slight activation
D-erythrose 4-phosphate
-
slight activation
D-fructose 6-phosphate
-
slight activation
additional information
-
no effectors of UDP-galactose-forming reaction are MgUDP, magnesium citrate, magnesium oxaloacetate or sucrose
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.415 - 1.2
alpha-D-galactose 1-phosphate
0.23 - 0.75
alpha-D-glucose 1-phosphate
0.18 - 0.22
diphosphate
0.37 - 0.47
UDP-galactose
0.034 - 0.14
UTP
additional information
additional information
-
steady-state kinetics of G, overview
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.58
Mg-diphosphate
-
pH 7.2, 30C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0025
-
healthy individuals
0.0027
-
galactosemic individuals
0.36
-
peduncles of developing fruits
0.56
-
unpollinated peduncles
3.8
-
partially purified enzyme, substrate alpha-D-galactose 1-phosphate
17
-
purified enzyme
135
-
purified enzyme
190.7
-
purified enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7.5
-
broad, forward reaction
6
-
reverse reaction
7.3 - 8.6
-
broad
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
; of unpollinated ovaries
Manually annotated by BRENDA team
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from healthy and galactosemic individuals
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
-
gel filtration
54000
-
gel filtration
200000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 68000, SDS-PAGE
tetramer
-
4 * 51000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
15 min, 10% loss of activity
47
-
t1/2: 10 min
50
-
15 min, 70% loss of activity, t1/2: 7.5 min, enzyme from healthy individual, and 10 min, enzyme from galactosemic individual
60
-
10 min, inactivation
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, in 20 mM imidazole-HCl buffer, pH 7, 20% v/v glycerol, 30% loss of activity within 6 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
113fold
-
partial
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
the GALT gene is located on chromosome 9p13
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A320T
-
the mutation causes classical galactosemia
IVS5nt-24G>A
-
higher risk of catarct for individuals
L218L
-
higher risk of catarct for individuals
N314D
-
higher risk of catarct for individuals
Q1883
-
higher risk of catarct for individuals
Q188R
-
the mutation causes classical galactosemia
S135L
-
the mutation causes classical galactosemia
Y209S
-
the mutation causes classical galactosemia
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diagnostics
-
the enzyme activity is measured for determination of galactosaemia using erythrocyte from blood samples, HPLC-based method development, overview
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