Information on EC 2.7.7.34 - glucose-1-phosphate guanylyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.7.7.34
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RECOMMENDED NAME
GeneOntology No.
glucose-1-phosphate guanylyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GTP + alpha-D-glucose 1-phosphate = diphosphate + GDP-glucose
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
GDP-glucose biosynthesis
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Starch and sucrose metabolism
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Amino sugar and nucleotide sugar metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
GTP:alpha-D-glucose-1-phosphate guanylyltransferase
Also acts, more slowly, on D-mannose 1-phosphate.
CAS REGISTRY NUMBER
COMMENTARY hide
9033-13-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
mast cell tumor, grown in LAF mice
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Manually annotated by BRENDA team
IFO 3439, IFO 3123
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Manually annotated by BRENDA team
IFO 3122, IFO 3430, IFO 3356
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Manually annotated by BRENDA team
IFO 3145
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Manually annotated by BRENDA team
IFO 3409
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Manually annotated by BRENDA team
IFO 3389
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Manually annotated by BRENDA team
IFO 3441
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Manually annotated by BRENDA team
IFO 3310
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Manually annotated by BRENDA team
IFO 3111
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Manually annotated by BRENDA team
strain AKU 2801
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Manually annotated by BRENDA team
strain AKU 2801
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-glucose
show the reaction diagram
GTP + D-mannose 1-phosphate
diphosphate + GDP-mannose
show the reaction diagram
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less effective than alpha-D-glucose 1-phosphate
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r
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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absolute requirement, highest activity at 8-10 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-mannose 1-phosphate
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0.07 mM: 18% inhibition in presence of 3.5 mM GDPglucose, 40% inhibition in presence of 0.7 mM GDPglucose, no inhibition in presence of more than 7 mM GDPglucose
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
diphosphate
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pH 7.4, 30°C
0.1
GDPglucose
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pH 7.4, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.046
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2 - 7.7
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8.7
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pH 6: 65% of maximal activity, pH 8.7: 60% of maximal activity at pH 8.7
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
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no activity in fibrosarcomase and umbilical cord
Manually annotated by BRENDA team
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, dried acetone powder, stable for at least two weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
mast cell tumor, grown in LAF mice, partial
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