Information on EC 2.7.7.40 - D-ribitol-5-phosphate cytidylyltransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.7.7.40
-
RECOMMENDED NAME
GeneOntology No.
D-ribitol-5-phosphate cytidylyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
CTP + D-ribitol 5-phosphate = diphosphate + CDP-ribitol
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
poly(ribitol phosphate) wall teichoic acid biosynthesis I (B. subtilis)
-
-
poly(ribitol phosphate) wall teichoic acid biosynthesis II (S. aureus)
-
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type IV lipoteichoic acid biosynthesis (S. pneumoniae)
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Pentose and glucuronate interconversions
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-
SYSTEMATIC NAME
IUBMB Comments
CTP:D-ribitol-5-phosphate cytidylyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9027-07-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CTP + D-arabitol 5-phosphate
diphosphate + CDParabitol
show the reaction diagram
-
-
-
-
?
CTP + D-ribitol 5-phosphate
diphosphate + CDP-ribitol
show the reaction diagram
CTP + D-ribitol 5-phosphate
diphosphate + CDPribitol
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CTP + D-ribitol 5-phosphate
diphosphate + CDPribitol
show the reaction diagram
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CDP-ribitol
diphosphate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00851 - 0.39
CTP
0.0147 - 1.28
D-ribitol 5-phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.8 - 76.8
CTP
0.14 - 13
D-ribitol 5-phosphate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15.7
-
CDP-ribitol pyrophosphorylase activity
37.5
-
ribitol 5-phosphate dehydrogenase activity
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.1
-
same enzyme activity at pH 7.1 and pH 8
8
-
same enzyme activity at pH 7.1 and pH 8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Bacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
53000
-
SDS-PAGE
106400
-
amino acid composition
129000
-
calculation from gel filtration and sedimentation velocity data
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to 1.78 A resolution. Space group C2, dimer in the asymmetric unit
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23
-
50% loss of activity after 2 h in 20 mM HEPES, 1 mM DTT and 0.5mg/ml bovine serum albumin, decrease of activity can be prevented by addition of 0.005 mM CTP
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
unstable, 50% loss of activity after 2 h at 23C in 20 mM HEPES, 1 mM DTT and 0.5mg/ml bovine serum albumin, decrease of activity can be prevented by addition of 0.05 mM CTP
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
enzyme copurifies with ribitol 5-phosphate dehydrogenase activity
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Hi Trap affinity nickel column chromatography and Superdex S200 gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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overexpression of TarI and TarJ individually from their respective genes using the T7 expression system
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K368A
-
kinetic values similar to wild-type enzyme
R18A
-
reduced enzyme activity