Information on EC 2.7.7.B3 - thiamine diphosphate adenylyl transferase

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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
2.7.7.B3
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
thiamine diphosphate adenylyl transferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
thiamine diphosphate + ADP = adenosine thiamine triphosphate + phosphate
show the reaction diagram
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thiamine diphosphate + ATP = adenosine thiamine triphosphate + diphosphate
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
ADP:thiamine diphosphate adenylyltransferase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
thiamine diphosphate + ADP
adenosine thiamine triphosphate + phosphate
show the reaction diagram
thiamine diphosphate + ATP
adenosine thiamine triphosphate + diphosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
thiamine diphosphate + ADP
adenosine thiamine triphosphate + phosphate
show the reaction diagram
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biosynthesis of adenosine thiamine triphosphate. Adenosine thiamine triphosphate appears to be a signal produced in bacteria in response to some form of cellular stress
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?
thiamine diphosphate + ATP
adenosine thiamine triphosphate + diphosphate
show the reaction diagram
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biosynthesis of adenosine thiamine triphosphate. Adenosine thiamine triphosphate appears to be a signal produced in bacteria in response to some form of cellular stress
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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5 mM, requirement for divalent metal ions, Mn2+ is most efficient
Mn2+
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5 mM, requirement for divalent metal ions, Mg2+ is 70% less effective than Mg2+
additional information
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5 mM Ca2+ has no effect on activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bis-Tris-propane
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
heat-stable soluble activator
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requirement for a heat-stable soluble activator present in bacterial extracts
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.2
thiamine diphosphate
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pH 6.5, 37C, cosubstrate: ADP
additional information
additional information
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varying the ADP concentration does not yield Michealis-Menten kinetics
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00008
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE