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Literature summary for 1.1.1.1 extracted from

  • Stroemberg, P.; Svensson, S.; Berst, K.B.; Plapp, B.V.; Höög, J.O.
    Enzymatic mechanism of low-activity mouse alcohol dehydrogenase 2 (2004), Biochemistry, 43, 1323-1328.
    View publication on PubMed
Search for more literature for 1.1.1.1

Protein Variants

Protein Variants Comment Organism
P47A site-directed mutagenesis, about 100fold increased activity compared to the wild-type enzyme Mus musculus
P47H site-directed mutagenesis, about 100fold increased activity compared to the wild-type enzyme Mus musculus
P47Q site-directed mutagenesis, about 100fold increased activity compared to the wild-type enzyme Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
cyclohexylformamide dead-end inhibition pattern Mus musculus
octanoic acid dead-end inhibition pattern Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ catalytic zinc ion Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q9QYY9 low-activity isozyme ADH2
-

Reaction

Reaction Comment Organism Reaction ID
a primary alcohol + NAD+ = an aldehyde + NADH + H+ ordered bibi mechanism, structural and functional implications of amino acid residue 47 Mus musculus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyl alcohol + NAD+
-
 Mus musculus benzaldehyde + NADH
-
 r
octanol + NAD+
-
 Mus musculus octanal + NADH
-
 ?

Synonyms

Synonyms Comment Organism
ADH2
-
 Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 enzyme is strongly pH-dependent Mus musculus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Mus musculus
NADH
-
 Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.026
-
 octanoic acid pH 7.5, 25°C, wild-type enzyme, versus NAD+ Mus musculus
0.035
-
 cyclohexylformamide pH 7.5, 25°C, wild-type enzyme, versus benzaldehyde Mus musculus
0.037
-
 octanoic acid pH 7.5, 25°C, wild-type enzyme, versus octanol Mus musculus
0.072
-
 cyclohexylformamide pH 7.5, 25°C, wild-type enzyme, versus NADH Mus musculus