Literature summary for 1.1.1.1 extracted from

Kosjek, B.; Stampfer, W.; Pogorevc, M.; Goessler, W.; Faber, K.; Kroutil, W.
Purification and characterization of a chemotolerant alcohol dehydrogenase applicable to coupled redox reactions (2004), Biotechnol. Bioeng., 86, 55-62.

Search for more literature for 1.1.1.1

Application

Application	Comment	Organism
synthesis	enzyme can be used in preparative scale enantioselective oxidation of sec-alcohol in asymmetric reduction of ketones, using acetone and 2-propanol, respectively, as cosubstrates for cofactor-regeneration via a coupled-substrate approach	Rhodococcus ruber

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	1 tightly bound ion per subunit	Rhodococcus ruber

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	2 * 38000, SDS-PAGE	Rhodococcus ruber

Organic Solvent Stability

Organic Solvent	Comment	Organism
2-propanol	80% v/v, stable at	Rhodococcus ruber
Acetone	50% v/v, stable at	Rhodococcus ruber
additional information	chemotolerant enzyme	Rhodococcus ruber

Organism

Organism	UniProt	Comment	Textmining
Rhodococcus ruber	-	strain DSM44541	-
Rhodococcus ruber DSM 44541	-	strain DSM44541	-

Purification (Commentary)

Purification (Comment)	Organism
-	Rhodococcus ruber

Reaction

Reaction	Comment	Organism	Reaction ID
a primary alcohol + NAD+ = an aldehyde + NADH + H+	sequential reaction mechanism	Rhodococcus ruber	a

Storage Stability

Storage Stability	Organism
very stable during storage	Rhodococcus ruber

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alcohol + NAD+	medium chain sec-alcohols or (omega-1)-ketones, no activity with primary alcohols or aldehydes	Rhodococcus ruber	aldehyde or ketone + NADH	-	r
alcohol + NAD+	medium chain sec-alcohols or (omega-1)-ketones, no activity with primary alcohols or aldehydes	Rhodococcus ruber DSM 44541	aldehyde or ketone + NADH	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 38000, SDS-PAGE	Rhodococcus ruber

Synonyms

Synonyms	Comment	Organism
medium-chain secondary alcohol dehydrogenase	-	Rhodococcus ruber
sec-ADH A	-	Rhodococcus ruber

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	50	-	Rhodococcus ruber

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	half-life: 35 h	Rhodococcus ruber

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	reduction	Rhodococcus ruber
9	-	oxidation	Rhodococcus ruber

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	dependent on	Rhodococcus ruber
NADH	dependent on	Rhodococcus ruber

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Release 2023.1 (January 2023)