Cloned (Comment) | Organism |
---|---|
- |
Candida parapsilosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, 99% loss of activity | Candida parapsilosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration | Candida parapsilosis |
31000 | - |
1 * 31000, SDS-PAGE | Candida parapsilosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida parapsilosis | B2KJ46 | fragment; CCTCC M203011 | - |
Purification (Comment) | Organism |
---|---|
- |
Candida parapsilosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-butanone + NADPH + H+ | 12.1% of the activity with 2-hydroxyacetophenone and NADPH | Candida parapsilosis | butan-2-ol + NADP+ | - |
? | |
2-heptanone + NADPH + H+ | 6.8% of the activity with 2-hydroxyacetophenone and NADPH | Candida parapsilosis | heptan-2-ol + NADP+ | - |
? | |
2-hexanone + NADPH + H+ | 5.9% of the activity with 2-hydroxyacetophenone and NADPH | Candida parapsilosis | hexan-2-ol + NADP+ | - |
? | |
2-hydroxyacetophenone + NADH + H+ | 2.4% of the activity with NADPH | Candida parapsilosis | (S)-1-phenyl-1,2-ethanediol + NAD+ | - |
? | |
2-hydroxyacetophenone + NADPH + H+ | - |
Candida parapsilosis | (S)-1-phenyl-1,2-ethanediol + NADP+ | - |
? | |
2-octanone + NADPH + H+ | 11.9% of the activity with 2-hydroxyacetophenone and NADPH | Candida parapsilosis | octan-2-ol + NADP+ | - |
? | |
2-pentanone + NADPH + H+ | 11.9% of the activity with 2-hydroxyacetophenone and NADPH | Candida parapsilosis | pentan-2-ol + NADP+ | - |
? | |
3-pentanone + NADPH + H+ | 7.1% of the activity with 2-hydroxyacetophenone and NADPH | Candida parapsilosis | pentan-3-ol + NADP+ | - |
? | |
acetone + NADPH + H+ | 14.7% of the activity with 2-hydroxyacetophenone and NADPH | Candida parapsilosis | propan-2-ol + NADP+ | - |
? | |
acetophenone + NADPH + H+ | 5.1% of the activity with 2-hydroxyacetophenone and NADPH | Candida parapsilosis | 1-phenylethanol + NADP+ | - |
? | |
benzoylformic acid + NADPH + H+ | 2.4% of the activity with 2-hydroxyacetophenone and NADPH | Candida parapsilosis | mandelate + NADP+ | - |
? | |
ethyl 4-chloroacetoacetate + NADPH | activity is 1.48fold higher than with 2-hydroxyacetophenone and NADPH | Candida parapsilosis | ? | - |
? | |
ethyl trifluoroacetoacetate + NADPH + H+ | 4.3% of the activity with 2-hydroxyacetophenone and NADPH | Candida parapsilosis | 1-ethoxy-2,2,2-trifluoroethanol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 31000, SDS-PAGE | Candida parapsilosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Candida parapsilosis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | 20°C: about 80% of maximal activity, 50°C: about 55% of maximal activity | Candida parapsilosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
60 min, stable up to | Candida parapsilosis |
50 | - |
60 min, about 50% loss of activity | Candida parapsilosis |
60 | - |
60 min, complete loss of activity | Candida parapsilosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Candida parapsilosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7.5 | pH 4.0: about 80% of maximal activity, pH 7.5: about 60% of maximal activity | Candida parapsilosis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | 4°C, 48 h, stable | Candida parapsilosis |