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Literature summary for 1.1.1.1 extracted from

  • Kannuchamy, S.; Mukund, N.; Saleena, L.M.
    Genetic engineering of Clostridium thermocellum DSM1313 for enhanced ethanol production (2016), BMC Biotechnol., 16, 34.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis enhancement of ethanol production capacity of Clostridium thermocellum by transferring pyruvate decarboxylase and alcohol dehydrogenase genes of the homoethanol pathway from Zymomonas mobilis. Both transferring pyruvate decarboxylase and alcohol dehydrogenase are functional in Clostridium thermocellum, but the presence of and alcohol dehydrogenase severely limits the growth of the recombinant strains, irrespective of the presence or absence of the pyruvate decarboxylase gene. The recombinant strain shows two-fold increase in pyruvate carboxylase activity and ethanol production when compared with the wild type strain Zymomonas mobilis subsp. mobilis
synthesis expression of pyruvate decarboxylase and alcohol dehydrogenase in Clostridium thermocellum DSM 1313. Though both enzymes are functional in Clostridium thermocellum, the presence of alcohol dehydrogenase severely limits the growth of the recombinant strains, irrespective of the presence or absence of the pyruvate decarboxylase gene Zymomonas mobilis subsp. mobilis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Clostridium thermocellum Zymomonas mobilis subsp. mobilis
expression in Clostridium thermocellum DSM 1313 Zymomonas mobilis subsp. mobilis

Organism

Organism UniProt Comment Textmining
Zymomonas mobilis subsp. mobilis P0DJA2
-
-
Zymomonas mobilis subsp. mobilis ATCC 31821 P0DJA2
-
-

Synonyms

Synonyms Comment Organism
AdhB
-
Zymomonas mobilis subsp. mobilis
alcohol dehydrogenase 2
-
Zymomonas mobilis subsp. mobilis

Cofactor

Cofactor Comment Organism Structure
NADH
-
Zymomonas mobilis subsp. mobilis