Protein Variants | Comment | Organism |
---|---|---|
additional information | mutations Y25A (at the dimer interface) and V260A (at the cofactor-binding domain) exhibit opposing low-temperature effects on the hydride tunneling step. The distal Y25A increases active-site flexibility, V260A introduces a temperature-dependent equilibration process, and the double mutant (Y25A/V260A) eliminates the temperature-dependent transition sensed by the active-site tryptophan in the presence of V260A. V260A displays a structural change in the active-site environment/solvation | Geobacillus stearothermophilus |
V260A | kinetic parameters and temperature dependencies similar to wild-type | Geobacillus stearothermophilus |
W49F/W167Y | kinetic parameters and temperature dependencies similar to wild-type | Geobacillus stearothermophilus |
W49F/W167Y/V260A | kinetic parameters and temperature dependencies similar to wild-type | Geobacillus stearothermophilus |
W49F/W87F | kinetic parameters and temperature dependencies similar to wild-type | Geobacillus stearothermophilus |
W49F/W87F/V260A | kinetic parameters and temperature dependencies similar to wild-type | Geobacillus stearothermophilus |
Y25A | kinetic parameters and temperature dependencies similar to wild-type | Geobacillus stearothermophilus |
Y25A/W49F/W167Y | kinetic parameters and temperature dependencies similar to wild-type | Geobacillus stearothermophilus |
Y25A/W49F/W167Y/V260A | kinetic parameters and temperature dependencies similar to wild-type | Geobacillus stearothermophilus |
Y25A/W49F/W87F | kinetic parameters and temperature dependencies similar to wild-type | Geobacillus stearothermophilus |
Y25A/W49F/W87F/V260A | kinetic parameters and temperature dependencies similar to wild-type | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
NAD+ | mutant Y25A/W49F/W167Y, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
0.8 | - |
benzyl alcohol | mutant Y25A/W49F/W167Y, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
0.9 | - |
NAD+ | mutant Y25A/W49F/W87F, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
1 | - |
NAD+ | mutant Y25A, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
1.1 | - |
NAD+ | wild-type, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
1.3 | - |
NAD+ | mutant W49F/W87F, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
1.8 | - |
NAD+ | mutant W49F/W167Y, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
3.1 | - |
benzyl alcohol | mutant W49F/W167Y, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
4.2 | - |
benzyl alcohol | mutant V260A, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
5.4 | - |
benzyl alcohol | mutant Y25A/W49F/W167Y/V260A, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
6 | - |
benzyl alcohol | mutant W49F/W87F, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
6.8 | - |
benzyl alcohol | wild-type, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
8.2 | - |
benzyl alcohol | mutant W49F/W167Y/V260A, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
8.3 | - |
NAD+ | mutant W49F/W167Y/V260A, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
9.7 | - |
NAD+ | mutant Y25A/W49F/W167Y/V260A, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
10 | - |
NAD+ | mutant V260A, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
10.1 | - |
benzyl alcohol | mutant Y25A/W49F/W87F/V260A, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
10.2 | - |
NAD+ | mutant W49F/W87F/V260A, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
10.9 | - |
benzyl alcohol | mutant Y25A/W49F/W87F, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
12.9 | - |
benzyl alcohol | mutant W49F/W87F/V260A, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
14.8 | - |
NAD+ | mutant Y25A/W49F/W87F/V260A, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
16.5 | - |
benzyl alcohol | mutant Y25A, 30°C, pH not specified in the publication | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | P42328 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyl alcohol + NAD+ | - |
Geobacillus stearothermophilus | benzaldehyde + NADH + H+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
NAD+ | mutant W49F/W87F/V260A, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
1.4 | - |
NAD+ | mutant W49F/W167Y/V260A, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
1.9 | - |
NAD+ | mutant V260A, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
1.9 | - |
NAD+ | mutant Y25A/W49F/W167Y/V260A, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
2.5 | - |
NAD+ | mutant Y25A/W49F/W167Y, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
2.5 | - |
NAD+ | mutant Y25A/W49F/W87F/V260A, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
3 | - |
NAD+ | mutant W49F/W87F, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
3.4 | - |
NAD+ | mutant W49F/W167Y, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
5.1 | - |
NAD+ | mutant Y25A, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
6.3 | - |
NAD+ | mutant Y25A/W49F/W87F, 30°C, pH not specified in the publication | Geobacillus stearothermophilus | |
8 | - |
NAD+ | wild-type, 30°C, pH not specified in the publication | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Geobacillus stearothermophilus |