Crystallization (Comment) | Organism |
---|---|
structural modeling of ethanol-tolerant mutant protein | Acetivibrio thermocellus |
Protein Variants | Comment | Organism |
---|---|---|
P704L/H734R | mutation leads to ethanol-tolerant phenotype. P704L may affect cofactor-binding directly, while mutation H734R is close to the active site iron atom. Mutant displays a complete loss of NADH-dependent activity with concomitant acquisition of NADPH-dependent activity | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | A3DCI2 | - |
- |
Acetivibrio thermocellus DSM 1237 | A3DCI2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NADH + H+ | - |
Acetivibrio thermocellus | ethanol + NAD+ | - |
r | |
acetaldehyde + NADH + H+ | - |
Acetivibrio thermocellus DSM 1237 | ethanol + NAD+ | - |
r | |
acetaldehyde + NADPH + H+ | NADPH is not a cofactor for wild-type, but for mutant P704L/H734R | Acetivibrio thermocellus | ethanol + NADP+ | - |
r | |
acetaldehyde + NADPH + H+ | NADPH is not a cofactor for wild-type, but for mutant P704L/H734R | Acetivibrio thermocellus DSM 1237 | ethanol + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AdhE | - |
Acetivibrio thermocellus |
Cthe_0423 | - |
Acetivibrio thermocellus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Acetivibrio thermocellus | |
NADPH | - |
Acetivibrio thermocellus |
General Information | Comment | Organism |
---|---|---|
physiological function | in ethanol-tolerant mutant strain, EA, the tolerant phenotype is primarily due to a mutated bifunctional acetaldehyde-CoA/alcohol dehydrogenase gene (adhE), with a complete loss of NADH-dependent activity and concomitant acquisition of NADPH-dependent activity, which likely affects electron flow in the mutant strain. Mutant adhE allele alone confers increased ethanol tolerance | Acetivibrio thermocellus |