KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NAD+ | cosubstrate butan-1-ol, pH 7.0, 30°C | Mycobacterium sp. | |
0.09 | - |
NADH | cosubstrate butanal, pH 7.0, 30°C | Mycobacterium sp. | |
0.48 | - |
Butanal | cosubstrate NADH, pH 7.0, 30°C | Mycobacterium sp. | |
5.65 | - |
butan-1-ol | cosubstrate NAD+, pH 7.0, 30°C | Mycobacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
- |
Mycobacterium sp. |
150000 | - |
gel filtration | Mycobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium sp. | W8VSK8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-pentanediol + NAD+ | 18% of the activity with butan-1-ol | Mycobacterium sp. | ? + NADH + H+ | - |
? | |
butan-1-ol + NAD+ | best substrate | Mycobacterium sp. | butanal + NADH + H+ | - |
r | |
butanal + NADH + H+ | - |
Mycobacterium sp. | butan-1-ol + NAD+ | - |
r | |
ethanol + NAD+ | 12% of the activity with butan-1-ol | Mycobacterium sp. | acetaldehyde + NADH + H+ | - |
r | |
additional information | enzyme shows activity as a reductase specific for (S)-acetoin, EC 1.1.1.76, and both diacetyl reductase (EC 1.1.1.304) and NAD+-dependent alcohol dehydrogenase (EC 1.1.1.1) activities | Mycobacterium sp. | ? | - |
? | |
propan-1-ol + NAD+ | 55% of the activity with butan-1-ol | Mycobacterium sp. | propanal + NADH + H+ | - |
r | |
propan-2-ol + NAD+ | 65% of the activity with butan-1-ol | Mycobacterium sp. | acetone + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 36000, SDS-PAGE, 4 * 35971, calculated | Mycobacterium sp. |
Synonyms | Comment | Organism |
---|---|---|
ADS1 | - |
Mycobacterium sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
NAD+ | cosubstrate butan-1-ol, pH 7.0, 30°C | Mycobacterium sp. | |
2 | - |
butan-1-ol | cosubstrate NAD+, pH 7.0, 30°C | Mycobacterium sp. | |
102 | - |
Butanal | cosubstrate NADH, pH 7.0, 30°C | Mycobacterium sp. | |
153 | - |
NADH | cosubstrate butanal, pH 7.0, 30°C | Mycobacterium sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Mycobacterium sp. | isoelectric focusing | - |
4.8 |
Mycobacterium sp. | calculated | - |
5.1 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
butan-1-ol | cosubstrate NAD+, pH 7.0, 30°C | Mycobacterium sp. | |
80 | - |
NAD+ | cosubstrate butan-1-ol, pH 7.0, 30°C | Mycobacterium sp. | |
210 | - |
Butanal | cosubstrate NADH, pH 7.0, 30°C | Mycobacterium sp. | |
2000 | - |
NADH | cosubstrate butanal, pH 7.0, 30°C | Mycobacterium sp. |