Cloned (Comment) | Organism |
---|---|
expression of Pichia stipitis mutated XYL2 (D207/I208R/F209S or XYL2 S96C/S99C/Y102C/D207A/I208R/F209S) in Saccharomyces cerevisiae | Scheffersomyces stipitis |
Protein Variants | Comment | Organism |
---|---|---|
D207/I208R/F209S | kcat/Km of mutant enzyme for NAD+ dropps 15fold compared with the native enzyme, kcat/Km for NADP+ increases up to 4100fold | Scheffersomyces stipitis |
S96C/S99C/Y102C/D207A/I208R/F209S | mutation produces a further 4fold improvement in the kcat/Km for NADP+ compared to mutant enzyme D207/I208R/F209S | Scheffersomyces stipitis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Scheffersomyces stipitis | - |
- |
- |