Protein Variants | Comment | Organism |
---|---|---|
A154T | similar thermal stability as wild-type enzyme | Escherichia coli |
A154T/E233K | temperature-sensitive phenoptype, reductase activity is much more thermolabile than the activity of the wild-type strain | Escherichia coli |
E233K | temperature-sensitive mutant enzyme does not allow growth of Escherichia coli at 42°C in complementation assay | Salmonella enterica subsp. enterica serovar Typhimurium |
E233K | temperature-sensitive phenoptype, reductase activity is much more thermolabile than the activity of the wild-type strain | Escherichia coli |
M125I/S233T | temperature-sensitive mutant enzyme does not allow growth of Escherichia coli at 42°C in complementation assay | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-ketoacyl-[acyl-carrier protein] + NADPH | Salmonella enterica subsp. enterica serovar Typhimurium | first reductive step in the elongation cycle of fatty acid biosynthesis | beta-hydroxyacyl-[acyl-carrier protein] + NADP+ | - |
? | |
beta-ketoacyl-[acyl-carrier protein] + NADPH | Escherichia coli | first reductive step in the elongation cycle of fatty acid biosynthesis | beta-hydroxyacyl-[acyl-carrier protein] + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
serovar typhimurium | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + NADPH + H+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | D-beta-hydroxybutyryl-CoA + NADP+ | - |
? | |
acetoacetyl-CoA + NADPH + H+ | - |
Escherichia coli | D-beta-hydroxybutyryl-CoA + NADP+ | - |
? | |
beta-ketoacyl-[acyl-carrier protein] + NADPH | first reductive step in the elongation cycle of fatty acid biosynthesis | Salmonella enterica subsp. enterica serovar Typhimurium | beta-hydroxyacyl-[acyl-carrier protein] + NADP+ | - |
? | |
beta-ketoacyl-[acyl-carrier protein] + NADPH | first reductive step in the elongation cycle of fatty acid biosynthesis | Escherichia coli | beta-hydroxyacyl-[acyl-carrier protein] + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoacyl-acyl carrier protein reductase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
beta-ketoacyl-acyl carrier protein reductase | - |
Escherichia coli |
FabG | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
FabG | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
wild-type enzyme: about 15% loss of activity after 15 min, mutant enzyme E233K: about 60% loss of activity after 10 min,mutant enzyme A164T: about 20% loss of activity after 15 min, mutant enzyme A154T/E233K: about 80% loss of activity after 15 min | Escherichia coli |
37 | - |
wild-type enzyme: about 40% loss of activity after 15 min, mutant enzyme E233K: about 90% loss of activity after 10 min, mutant enzyme A164T: about 25% loss of activity after 15 min, mutant enzyme A154T/E233K: about 80% loss of activity after 5 min | Escherichia coli |
45 | - |
wild-type enzyme: about 70% loss of activity after 15 min, mutant enzyme E233K: complete loss of activity after 10 min, mutant enzyme A164T: about 60% loss of activity after 15 min, mutant enzyme A154T/E233K: complete loss of activity after 5 min | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
NADPH | - |
Escherichia coli |