Cloned (Comment) | Organism |
---|---|
gene fabG, overexpression of His-tagged enzyme in strain BL21(DE3) | Escherichia coli |
gene fabG, overexpression of His-tagged enzyme in strain BL21(DE3), recombinant expression in Escherichia coli strain JM109 leads to enhanced production of polyhydroxyalkanoate, PHA, copolymer from glucose, PHA composition, overview | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene fabG | - |
Pseudomonas sp. | Q4AE87 | strain 61-3, non-pathogenic strain from soil, gene fabG | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography | Escherichia coli |
recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-oxodecanoyl-CoA + NADPH | - |
Escherichia coli | (R)-3-hydroxydecanoyl-CoA + NADP+ | - |
? | |
3-oxodecanoyl-CoA + NADPH | - |
Pseudomonas sp. | (R)-3-hydroxydecanoyl-CoA + NADP+ | - |
? | |
3-oxododecanoyl-CoA + NADPH | - |
Escherichia coli | (R)-3-hydroxydodecanoyl-CoA + NADP+ | - |
? | |
3-oxododecanoyl-CoA + NADPH | - |
Pseudomonas sp. | (R)-3-hydroxydodecanoyl-CoA + NADP+ | - |
? | |
3-oxohexanoyl-CoA + NADPH | - |
Escherichia coli | (R)-3-hydroxyhexanoyl-CoA + NADP+ | - |
? | |
3-oxohexanoyl-CoA + NADPH | - |
Pseudomonas sp. | (R)-3-hydroxyhexanoyl-CoA + NADP+ | - |
? | |
3-oxooctanoyl-CoA + NADPH | - |
Escherichia coli | (R)-3-hydroxyoctanoyl-CoA + NADP+ | - |
? | |
3-oxooctanoyl-CoA + NADPH | - |
Pseudomonas sp. | (R)-3-hydroxyoctanoyl-CoA + NADP+ | - |
? | |
acetoacetyl-CoA + NADPH | - |
Escherichia coli | 3-hydroxybutyryl-CoA + NADP+ | - |
? | |
acetoacetyl-CoA + NADPH | - |
Pseudomonas sp. | 3-hydroxybutyryl-CoA + NADP+ | - |
? | |
additional information | FabG substrate specificity overview | Escherichia coli | ? | - |
? | |
additional information | FabG substrate specificity overview | Pseudomonas sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-ketoacyl-acyl carrier protein reductase | - |
Escherichia coli |
3-ketoacyl-acyl carrier protein reductase | - |
Pseudomonas sp. |
FabG | - |
Escherichia coli |
FabG | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Escherichia coli |
22 | - |
assay at room temperature | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
8 | - |
assay at | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Escherichia coli | |
NADPH | - |
Pseudomonas sp. |