Cloned (Comment) | Organism |
---|---|
gene fabG, DNA and amino acid sequence determination and analysis, expression of His10-tagged enzyme in Escherichia coli | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, vapour diffusion method, 10 mg/ml protein in 20 mM HEPES, pH 6.8, 0.5 M NaCl, 1 mM DTT, and 0.5 mM EDTA, is mixed with an equal volume of reservoir solution containing 0.1 M MES, pH 6.0, 35% v/v 2-methyl-2,4-pentanediol, and 0.2 M LiSO4, equilibration against 1 ml of mother liquor, room temperature, 6 weeks, X-ray diffraction structure determination and analysis at 1.9 A resolution | Plasmodium falciparum |
General Stability | Organism |
---|---|
the purified recombinant enzyme is stable at room temperature at concentrations above 0.2 mg/ml, but unstable at 0.01 mg/ml | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-hydroxybutyryl-CoA | competitive product inhibition | Plasmodium falciparum | |
bithionol | over 75% inhibition at 0.02 mM, IC50: 0.010 mM | Plasmodium falciparum | |
bromochlorophen | an anthelmintic agent, over 75% inhibition at 0.02 mM, IC50: 0.0154 mM | Plasmodium falciparum | |
di-resorcinol sulfide | over 75% inhibition at 0.02 mM, IC50: 0.0038 mM | Plasmodium falciparum | |
Hexachlorophene | an anthelmintic and antimicrobial agent, over 75% inhibition at 0.02 mM, IC50: 0.002 mM | Plasmodium falciparum | |
additional information | no inhibition by triclosan | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis of the recombinant enzyme, kinetic mechanism | Plasmodium falciparum | |
0.0475 | - |
NADPH | pH 6.8, 25°C, recombinant enzyme | Plasmodium falciparum | |
0.51 | - |
acetoacetyl-CoA | pH 6.8, 25°C, recombinant enzyme | Plasmodium falciparum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme activity is maximal at a ionic strength of 325 mM | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q86RB1 | gene fabG | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | the enzyme contains a signal peptide | Plasmodium falciparum |
Purification (Comment) | Organism |
---|---|
recombinant His10-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration | Plasmodium falciparum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+ | ordered bi bi kinetic mechanism, [acyl-carrier protein] recognition mechanism | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + NADPH | - |
Plasmodium falciparum | 3-hydroxybutyryl-CoA + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | crystal structure and ultrafiltration, quarternary structure analysis, monomer interactions, conformations in absence and presence of cofactor NADP+ | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
3-oxoacyl-ACP reductase | - |
Plasmodium falciparum |
FabG | - |
Plasmodium falciparum |
OAR | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
265 | - |
NADPH | pH 6.8, 25°C, recombinant enzyme | Plasmodium falciparum | |
265 | - |
acetoacetyl-CoA | pH 6.8, 25°C, recombinant enzyme | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | cofactor binding structure and mechanism | Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0102 | - |
3-hydroxybutyryl-CoA | pH 6.8, 25°C, recombinant enzyme | Plasmodium falciparum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.002 | - |
an anthelmintic and antimicrobial agent, over 75% inhibition at 0.02 mM, IC50: 0.002 mM | Plasmodium falciparum | Hexachlorophene | |
0.0038 | - |
over 75% inhibition at 0.02 mM, IC50: 0.0038 mM | Plasmodium falciparum | di-resorcinol sulfide | |
0.01 | - |
over 75% inhibition at 0.02 mM, IC50: 0.010 mM | Plasmodium falciparum | bithionol | |
0.0154 | - |
an anthelmintic agent, over 75% inhibition at 0.02 mM, IC50: 0.0154 mM | Plasmodium falciparum | bromochlorophen |