Cloned (Comment) | Organism |
---|---|
PCR product recombined with pDONR221 and insert from this vector transferred in the LR reaction to the expression vector pET15g which adds a histidine tag and a 3C protease cleavage site, expressed in Escherichia coli B834(DE3) cells | Bacillus anthracis |
Crystallization (Comment) | Organism |
---|---|
at 2.4 A resolution, space group P21 with unit-cell parameters a = 70.6, b = 120.7, c = 136.4 and beta = 104.4. The structure contains two tetramers displaying 222 symmetry (all chains are completely traced, although for some chains the electron density for residues 189-203 is poor) and 575 water molecules in the crystallographic asymmetric unit, but no bound cofactors or substrates | Bacillus anthracis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | Q81JG6 | - |
- |
Purification (Comment) | Organism |
---|---|
by nickel-affinity chromatography | Bacillus anthracis |
Subunits | Comment | Organism |
---|---|---|
tetramer | crystallography | Bacillus anthracis |
Synonyms | Comment | Organism |
---|---|---|
3-oxoacyl-(acyl carrier protein) reductase | - |
Bacillus anthracis |