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Literature summary for 1.1.1.102 extracted from

  • Fornarotto, M.; Xiao, L.; Hou, Y.; Koch, K.A.; Chang, E.; OMalley, R.M.; Black, T.A.; Cable, M.B.; Walker, S.S.
    Sphingolipid biosynthesis in pathogenic fungi: identification and characterization of the 3-ketosphinganine reductase activity of Candida albicans and Aspergillus fumigatus (2006), Biochim. Biophys. Acta, 1761, 52-63.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene ksr1, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain JM109(DE3) Candida albicans
gene ksrA, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain JM109(DE3) Aspergillus fumigatus
gene tsc10, expression of mutant tscDELTA38 in Escherichia coli strain BL21(DE3) Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information construction of an enzyme-deficient mutant by gene replacement, the mutant produces lower levels of inositolphosphorylceramides Candida albicans
additional information construction of the deletion mutant TscDELTA38p Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information binding kinetics of substrate analogues with shorter chain lengths Candida albicans
0.009
-
3-dehydrosphinganine pH 7.5, recombinant enzyme Candida albicans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-dehydrosphinganine + NADPH Aspergillus fumigatus
-
sphinganine + NADP+
-
?
3-dehydrosphinganine + NADPH Saccharomyces cerevisiae the enzyme catalyzes an early step in the sphingolipid biosynthesis, overview sphinganine + NADP+
-
?
3-dehydrosphinganine + NADPH Candida albicans the enzyme is not essential for cell viability, but enzyme-deficient mutants produce lower levels of inositolphosphorylceramides sphinganine + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
gene ksrA
-
Candida albicans
-
gene ksr1
-
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-dehydrosphinganine + NADPH
-
Saccharomyces cerevisiae sphinganine + NADP+
-
?
3-dehydrosphinganine + NADPH
-
Aspergillus fumigatus sphinganine + NADP+
-
?
3-dehydrosphinganine + NADPH the enzyme catalyzes an early step in the sphingolipid biosynthesis, overview Saccharomyces cerevisiae sphinganine + NADP+
-
?
3-dehydrosphinganine + NADPH the enzyme is not essential for cell viability, but enzyme-deficient mutants produce lower levels of inositolphosphorylceramides Candida albicans sphinganine + NADP+
-
?
3-dehydrosphinganine + NADPH C18 substrate, molecular modeling of substrate binding, overview Candida albicans sphinganine + NADP+
-
?

Subunits

Subunits Comment Organism
More molecular modeling of the enzyme with bound substrates, a significant portion of the aliphatic chain of 3-dehydrosphinganine protrudes from the enzyme, overview Candida albicans

Synonyms

Synonyms Comment Organism
3-ketosphinganine reductase
-
Saccharomyces cerevisiae
3-ketosphinganine reductase
-
Candida albicans
3-ketosphinganine reductase
-
Aspergillus fumigatus
Ksr1p
-
Candida albicans
KsrA
-
Aspergillus fumigatus
More the enzyme belongs to the short-chain dehydrogenase/reductase family of enzymes Saccharomyces cerevisiae
More the enzyme belongs to the short-chain dehydrogenase/reductase family of enzymes Candida albicans
More the enzyme belongs to the short-chain dehydrogenase/reductase family of enzymes Aspergillus fumigatus
TSC10
-
Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.07
-
3-dehydrosphinganine pH 7.5, recombinant enzyme Candida albicans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Saccharomyces cerevisiae
7.5
-
assay at Candida albicans
7.5
-
assay at Aspergillus fumigatus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Saccharomyces cerevisiae
NADPH
-
Candida albicans
NADPH
-
Aspergillus fumigatus