Cloned (Comment) | Organism |
---|---|
gene ksr1, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain JM109(DE3) | Candida albicans |
gene ksrA, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain JM109(DE3) | Aspergillus fumigatus |
gene tsc10, expression of mutant tscDELTA38 in Escherichia coli strain BL21(DE3) | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an enzyme-deficient mutant by gene replacement, the mutant produces lower levels of inositolphosphorylceramides | Candida albicans |
additional information | construction of the deletion mutant TscDELTA38p | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | binding kinetics of substrate analogues with shorter chain lengths | Candida albicans | |
0.009 | - |
3-dehydrosphinganine | pH 7.5, recombinant enzyme | Candida albicans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-dehydrosphinganine + NADPH | Aspergillus fumigatus | - |
sphinganine + NADP+ | - |
? | |
3-dehydrosphinganine + NADPH | Saccharomyces cerevisiae | the enzyme catalyzes an early step in the sphingolipid biosynthesis, overview | sphinganine + NADP+ | - |
? | |
3-dehydrosphinganine + NADPH | Candida albicans | the enzyme is not essential for cell viability, but enzyme-deficient mutants produce lower levels of inositolphosphorylceramides | sphinganine + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | - |
gene ksrA | - |
Candida albicans | - |
gene ksr1 | - |
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-dehydrosphinganine + NADPH | - |
Saccharomyces cerevisiae | sphinganine + NADP+ | - |
? | |
3-dehydrosphinganine + NADPH | - |
Aspergillus fumigatus | sphinganine + NADP+ | - |
? | |
3-dehydrosphinganine + NADPH | the enzyme catalyzes an early step in the sphingolipid biosynthesis, overview | Saccharomyces cerevisiae | sphinganine + NADP+ | - |
? | |
3-dehydrosphinganine + NADPH | the enzyme is not essential for cell viability, but enzyme-deficient mutants produce lower levels of inositolphosphorylceramides | Candida albicans | sphinganine + NADP+ | - |
? | |
3-dehydrosphinganine + NADPH | C18 substrate, molecular modeling of substrate binding, overview | Candida albicans | sphinganine + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | molecular modeling of the enzyme with bound substrates, a significant portion of the aliphatic chain of 3-dehydrosphinganine protrudes from the enzyme, overview | Candida albicans |
Synonyms | Comment | Organism |
---|---|---|
3-ketosphinganine reductase | - |
Saccharomyces cerevisiae |
3-ketosphinganine reductase | - |
Candida albicans |
3-ketosphinganine reductase | - |
Aspergillus fumigatus |
Ksr1p | - |
Candida albicans |
KsrA | - |
Aspergillus fumigatus |
More | the enzyme belongs to the short-chain dehydrogenase/reductase family of enzymes | Saccharomyces cerevisiae |
More | the enzyme belongs to the short-chain dehydrogenase/reductase family of enzymes | Candida albicans |
More | the enzyme belongs to the short-chain dehydrogenase/reductase family of enzymes | Aspergillus fumigatus |
TSC10 | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.07 | - |
3-dehydrosphinganine | pH 7.5, recombinant enzyme | Candida albicans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |
7.5 | - |
assay at | Candida albicans |
7.5 | - |
assay at | Aspergillus fumigatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Saccharomyces cerevisiae | |
NADPH | - |
Candida albicans | |
NADPH | - |
Aspergillus fumigatus |