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Literature summary for 1.1.1.126 extracted from

  • Preiss, J.; Ashwell, G.
    Alginic acid metabolism in bacteria. II. The enzymatic reduction of 4-deoxy-L-erythro-5-hexoseulose uronic acid to 2-keto-3-deoxy-D-gluconic acid (1962), J. Biol. Chem., 237, 317-321.
    View publication on PubMed

General Stability

General Stability Organism
unstable in the absence of NADP+ Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
p-hydroxymercuribenzoate 26% inhibition at 0.1 mM Pseudomonas sp.
Tris-HCl 67% inhibition at pH 7.5, 50 mM Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
NADPH
-
Pseudomonas sp.
0.36
-
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate
-
Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADPH Pseudomonas sp. irreversible at pH 7.0, 1% reversibility at pH 10.0 2-dehydro-3-deoxy-D-gluconate + NADP+
-
ir

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
alginate-adapted
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture inducible enzyme synthesis by growth in alginate-containing medium Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.9
-
-
Pseudomonas sp.

Storage Stability

Storage Stability Organism
0-3°C, 1 mM NADP+, 90% retaining activity after 3 weeks Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADPH irreversible at pH 7.0, 1% reversibility at pH 10.0 Pseudomonas sp. 2-dehydro-3-deoxy-D-gluconate + NADP+
-
ir

Synonyms

Synonyms Comment Organism
ketodeoxygluconate dehydrogenase
-
Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NADH 7.2% of the rate compared to NADPH Pseudomonas sp.
NADPH
-
Pseudomonas sp.