Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | tetramethylammonium, dimethylammonium or sodium slats do not substitute for NH4+ or K+ | Pseudomonas aeruginosa | |
NH4+ | or K+, required | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | positive cooperativity with respect to substrate binding, Hill coefficient 1.8 | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | or NH4+, required | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50300 | - |
3 * 50300, SDS-PAGE | Pseudomonas aeruginosa |
153000 | - |
dynamic light scattering | Pseudomonas aeruginosa |
162000 | - |
gel filtration | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein with His-tag | Pseudomonas aeruginosa |
Storage Stability | Organism |
---|---|
-20°C, 25% glycerol, stable for months | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + 2 NAD+ + H2O | - |
Pseudomonas aeruginosa | UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + 2 NADH + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 50300, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
WbpA | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
33 | 37 | - |
Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas aeruginosa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas aeruginosa | isoelectric focusing | - |
5.5 |
Pseudomonas aeruginosa | predicted from gene sequence | - |
6 |