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Literature summary for 1.1.1.138 extracted from

  • Lee, J.; Oh, D.; Song, H.; Kim, I.
    Ca2+ and Cu2+ supplementation increases mannitol production by Candida magnoliae (2007), Biotechnol. Lett., 29, 291-294.
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme of strain strain HH-01, KCCM-10252, is useful in production of D-mannitol Starmerella magnoliae

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ increases the intracellular activity of mannitol dehydrogenase 1.6fold Starmerella magnoliae
additional information Ca2+ reduces the intracellular mannitol concetration by 30% probably by altering the permeability of cells for mannito Starmerella magnoliae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-mannitol + NADP+ Starmerella magnoliae
-
D-fructose + NADPH + H+
-
r
D-mannitol + NADP+ Starmerella magnoliae HH-01
-
D-fructose + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Starmerella magnoliae
-
-
-
Starmerella magnoliae HH-01
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information optimization of culture conditions, overview, supplementation with CaCl2 x 2 H2O at 50 mg/l or CuSO4+ x 5 H2O at 10 mg/l improves mannitol production by Candida magnoliae by 14.5 and 18.6% to 25 and 32 g/l, respectively Starmerella magnoliae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5 0.9 crude enzyme extract, optimization of culture conditions, overview Starmerella magnoliae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-mannitol + NADP+
-
Starmerella magnoliae D-fructose + NADPH + H+
-
r
D-mannitol + NADP+
-
Starmerella magnoliae HH-01 D-fructose + NADPH + H+
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Starmerella magnoliae

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Starmerella magnoliae
NADPH
-
Starmerella magnoliae