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Literature summary for 1.1.1.140 extracted from

  • Horwitz, S.B.
    D-mannitol 1-phosphate dehydrogenase and D-sorbitol 6-phosphate dehydrogenase from Aerobacter aerogenes (1966), Methods Enzymol., 9, 150-155.
No PubMed abstract available

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
enzyme induced by growth on D-sorbitol
-

Purification (Commentary)

Purification (Comment) Organism
approximately 100 fold purification Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Klebsiella aerogenes

Storage Stability

Storage Stability Organism
frozen, several weeks Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-dulcitol 6-phosphate + NAD+ at 3% of reaction reaction with sorbitol 6-phosphate Klebsiella aerogenes ?
-
?
D-sorbitol 6-phosphate + NAD+ high specificity for NAD+ Klebsiella aerogenes D-fructose 6-phosphate + NADH + H+
-
r
D-sorbitol 6-phosphate + NAD+ high specificity for D-sorbitol 6-phosphate Klebsiella aerogenes D-fructose 6-phosphate + NADH + H+
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
Tris-HCl buffer Klebsiella aerogenes

Cofactor

Cofactor Comment Organism Structure
NAD+ specific for Klebsiella aerogenes
NAD+ inhibited by alteration of the 6-amino position of the adenine moiety of NAD+ Klebsiella aerogenes