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Literature summary for 1.1.1.140 extracted from
- Purification and properties of D-mannitol-1-phosphate dehydrogenase and D-glucitol-6-phosphate dehydrogenase from Escherichia coli (1984), J. Bacteriol., 159, 986-990.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning and subcloning of the gut operon and gutD gene | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethyl dicarbonate | 100% inactivation | Escherichia coli |  |
| N-ethylmaleimide | about 90% inactivation | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
NAD+ | - |
Escherichia coli | |
| 3.3 | - |
D-sorbitol 6-phosphate | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
4 * 26000, SDS-PAGE | Escherichia coli |
| 117000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli LJ378 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 180 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, more than 4 months, no appreciable loss of activity | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-sorbitol 6-phosphate + NAD+ | high specificity for NAD+ | Escherichia coli | D-fructose 6-phosphate + NADH + H+ | - |
? | |
| D-sorbitol 6-phosphate + NAD+ | high specificity for D-sorbitol 6-phosphate | Escherichia coli | D-fructose 6-phosphate + NADH + H+ | - |
? | |
| D-sorbitol 6-phosphate + NAD+ | high specificity for NAD+ | Escherichia coli LJ378 | D-fructose 6-phosphate + NADH + H+ | - |
? | |
| D-sorbitol 6-phosphate + NAD+ | high specificity for D-sorbitol 6-phosphate | Escherichia coli LJ378 | D-fructose 6-phosphate + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 26000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay at room temperature | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
20 min, 85% loss of activity | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.7 | - |
- |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activity with NADP+ | Escherichia coli | |
| NAD+ | specific for | Escherichia coli | |
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