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Literature summary for 1.1.1.140 extracted from

  • Roux, C.; Salmon, L.; Verchere-Beaur, C.
    Preliminary studies on the inhibition of D-sorbitol-6-phosphate 2-dehydrogenase from Escherichia coli with substrate analogues (2006), J. Enzyme Inhib. Med. Chem., 21, 187-192.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene Ecs6pdh, overexpression in strain BL21 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
5-phospho-D-arabinonate substrate analogue inhibitor, IC50: 0.048 mM Escherichia coli
5-phospho-D-arabinonohydroxamic acid substrate analogue inhibitor, IC50: 0.040 mM Escherichia coli
D-mannose 6-phosphate potent inhibitor, IC50: 0.0075 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
NADH pH 7.5, 25°C, recombinant enzyme Escherichia coli
0.568
-
D-fructose 6-phosphate pH 7.5, 25°C, recombinant enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose 6-phosphate + NADH Escherichia coli
-
D-sorbitol 6-phosphate + NAD+
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from strain BL21 by ammonium sulfate fractionation and gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 6-phosphate + NADH
-
Escherichia coli D-sorbitol 6-phosphate + NAD+
-
r

Synonyms

Synonyms Comment Organism
D-sorbitol-6-phosphate 2-dehydrogenase
-
Escherichia coli
S6PDH
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Escherichia coli
NADH
-
Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0075
-
potent inhibitor, IC50: 0.0075 mM Escherichia coli D-mannose 6-phosphate
0.04
-
substrate analogue inhibitor, IC50: 0.040 mM Escherichia coli 5-phospho-D-arabinonohydroxamic acid
0.048
-
substrate analogue inhibitor, IC50: 0.048 mM Escherichia coli 5-phospho-D-arabinonate