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Literature summary for 1.1.1.141 extracted from

  • Jabarak, J.
    Isolation and properties of an NAD+-dependent 15-hydroxyprostaglandin dehydrogenase from human placenta (1982), Methods Enzymol., 86, 126-130.
    View publication on PubMed
Search for more literature for 1.1.1.141

Activating Compound

Activating Compound Comment Organism Structure
Thionicotinamide adenine dinucleotide 5-33% of the activity with NAD+, depending on substrate Homo sapiens

General Stability

General Stability Organism
glycerol, 50%, stabilizes Homo sapiens
NAD+ stabilizes Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
arachidonic acid
-
 Homo sapiens
Ethacrynic acid
-
 Homo sapiens
Prostaglandin B1
-
 Homo sapiens
Prostanoic acid
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
 prostaglandin E1
-
 Homo sapiens
0.0048
-
 prostaglandin E1
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 1 * 42000, SDS-PAGE Homo sapiens
51500
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
15-hydroxyprostaglandin + NAD+ Homo sapiens physiological inactivation of prostaglandin by catalyzing the first step in catabolism 15-oxoprostaglandin + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
(5Z,13E,15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ = (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ single displacement mechanism Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.75
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, 5 mM potassium phosphate, pH 7.0, 50% glycerol, 1 mM EDTA, 7 months, 10% loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ i.e. prostaglandin E1 Homo sapiens (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+
-
 ?
11alpha,15(S)-dihydroxy-9-ketoprost-5,13-dienoic acid + NAD(P)+ specific for NAD+ Homo sapiens 11alpha-hydroxy-9,15-diketoprost-5,13-dienoic acid + NADH
-
 ?
15-hydroxyprostaglandin + NAD+ physiological inactivation of prostaglandin by catalyzing the first step in catabolism Homo sapiens 15-oxoprostaglandin + NADH + H+
-
 ?
6-ketoprostaglandin F1alpha + NAD+
-
 Homo sapiens 6,15-diketoprostaglandin F1alpha + NADH + H+
-
 ?
prostaglandin A1 + NAD+
-
 Homo sapiens 15-ketoprostaglandin A1 + NADH + H+
-
 ?
prostaglandin D1 + NAD+ very low activity Homo sapiens 15-ketoprostaglandin D1 + NADH + H+
-
 ?
prostaglandin E1 + NAD+
-
 Homo sapiens 15-ketoprostaglandin E1 + NADH + H+
-
 ?
prostaglandin F2alpha + NAD+ specific for NAD+ Homo sapiens 15-ketoprostaglandin F2alpha + NADH + H+
-
 ?
prostaglandin I2 + NAD+
-
 Homo sapiens 15-ketoprostaglandin I2 + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 42000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
additional information acetylpyridine dinucleotide can not replace NAD+ Homo sapiens
NAD+ specific for NAD+ Homo sapiens
NADH
-
 Homo sapiens
thio-NAD+ 5-33% of activity with NAD+ depending on substrate Homo sapiens