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Literature summary for 1.1.1.146 extracted from

  • Monder, C.; Lakshmi, V.; Miroff, Y.
    Kinetic studies on rat liver 11beta-hydroxysteroid dehydrogenase (1991), Biochim. Biophys. Acta, 1115, 23-29.
    View publication on PubMed
Search for more literature for 1.1.1.146

General Stability

General Stability Organism
NADP+ protects enzyme activity Rattus sp.

Inhibitors

Inhibitors Comment Organism Structure
3beta-hydroxy-11-oxo-18beta-olean-12-en-30-oic-acid competitive inhibition, Ki: 4 nM; i.e. glycyrrhetinic acid Rattus sp.
glycyrrhizic acid competitive inhibition, Ki: 0.0005 mM Rattus sp.
additional information no end-product inhibition Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000196
-
 NADP+ reduction of corticosterone, ordered sequential mechanism Rattus sp.
0.00183
-
 11beta,21-dihydroxypregn-4-en-3,20-dione
-
 Rattus sp.
0.00355
-
 NADPH oxidation of cortisol Rattus sp.
0.0173
-
 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione i.e. cortisol Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ Rattus sp. i.e. cortisol 17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH i.e. cortisone ir
21-hydroxy-pregn-4-en-3,11,20-trione + NADPH Rattus sp.
-
 11beta,21-dihydroxypregn-4-en-3,20-dione + NADP+ i.e. corticosterone ir

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus sp.
-

Storage Stability

Storage Stability Organism
-20°C, 1 mM NADP+, several months Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ i.e. cortisol Rattus sp. 17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH i.e. cortisone ir
21-hydroxy-pregn-4-en-3,11,20-trione + NADPH
-
 Rattus sp. 11beta,21-dihydroxypregn-4-en-3,20-dione + NADP+ i.e. corticosterone ir

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Rattus sp.
NADPH
-
 Rattus sp.