Cloned (Comment) | Organism |
---|---|
expression of His-tagged 11beta-HSD1 in Pichia pastoris | Homo sapiens |
expression of N-terminally His6-tagged isozyme 11beta-HSD1 in Pichia pastoris strain GS115 membranes | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
membrane | membrane bound | Homo sapiens | 16020 | - |
microsome | - |
Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cortisone + NADPH + H+ | Homo sapiens | primarly a reductase in vivo | cortisol + NADP+ | - |
r | |
cortisone + NADPH + H+ | Homo sapiens | isozyme 11beta-HSD1 | cortisol + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | - |
isozyme 11beta-HSD1 | - |
Purification (Comment) | Organism |
---|---|
affinity partitioning of His-tagged 11beta-HSD1 in polymer/detergent aqueous two-phase system | Homo sapiens |
recombinant His6-tagged isozyme 11beta-HSD1 from membrane by microsome preparation, in situ solubilization, partitioning and batch metal affinity adsorption in aqueous two-phase systems, optimization of solubilization using different detergents at 1% w/v, overview | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Hep-G2 cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.44 | - |
purified recombinant His6-tagged isozyme 11beta-HSD1 | Homo sapiens |
3.84 | - |
purified by detergent/polymer two-phase system, i.e. Tween20/dextran 500 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cortisol + NADP+ | - |
Homo sapiens | cortisone + NADPH | - |
r | |
cortisone + NADPH + H+ | primarly a reductase in vivo | Homo sapiens | cortisol + NADP+ | - |
r | |
cortisone + NADPH + H+ | isozyme 11beta-HSD1 | Homo sapiens | cortisol + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
11beta-HSD1 | - |
Homo sapiens |
11beta-hydroxysteroid dehydrogenase type 1 | - |
Homo sapiens |
isozyme 11beta-HSD1 | - |
Homo sapiens |
type 1 11beta-hydroxysteroid dehydrogenase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |