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Literature summary for 1.1.1.146 extracted from

  • Roobol-Boza, M.; Dolby, V.; Doverskog, M.; Barrefelt, A.; Lindqvist, F.; Oppermann, U.C.; Kohler Van Alstine, K.; Tjerneld, F.
    Membrane protein isolation by in situ solubilization, partitioning and affinity adsorption in aqueous two-phase systems. Purification of the human type 1 11beta-hydroxysteroid dehydrogenase (2004), J. Chromatogr. A, 1043, 217-223.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged 11beta-HSD1 in Pichia pastoris Homo sapiens
expression of N-terminally His6-tagged isozyme 11beta-HSD1 in Pichia pastoris strain GS115 membranes Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Homo sapiens 16020
-
membrane membrane bound Homo sapiens 16020
-
microsome
-
Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cortisone + NADPH + H+ Homo sapiens primarly a reductase in vivo cortisol + NADP+
-
r
cortisone + NADPH + H+ Homo sapiens isozyme 11beta-HSD1 cortisol + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens
-
isozyme 11beta-HSD1
-

Purification (Commentary)

Purification (Comment) Organism
affinity partitioning of His-tagged 11beta-HSD1 in polymer/detergent aqueous two-phase system Homo sapiens
recombinant His6-tagged isozyme 11beta-HSD1 from membrane by microsome preparation, in situ solubilization, partitioning and batch metal affinity adsorption in aqueous two-phase systems, optimization of solubilization using different detergents at 1% w/v, overview Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
Hep-G2 cell
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.44
-
purified recombinant His6-tagged isozyme 11beta-HSD1 Homo sapiens
3.84
-
purified by detergent/polymer two-phase system, i.e. Tween20/dextran 500 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cortisol + NADP+
-
Homo sapiens cortisone + NADPH
-
r
cortisone + NADPH + H+ primarly a reductase in vivo Homo sapiens cortisol + NADP+
-
r
cortisone + NADPH + H+ isozyme 11beta-HSD1 Homo sapiens cortisol + NADP+
-
r

Synonyms

Synonyms Comment Organism
11beta-HSD1
-
Homo sapiens
11beta-hydroxysteroid dehydrogenase type 1
-
Homo sapiens
isozyme 11beta-HSD1
-
Homo sapiens
type 1 11beta-hydroxysteroid dehydrogenase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Homo sapiens
NADPH
-
Homo sapiens