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Literature summary for 1.1.1.149 extracted from

  • Zhang, Y.; Dufort, I.; Rheault, P.; Luu-The, V.
    Characterization of a human 20alpha-hydroxysteroid dehydrogenase (2000), J. Mol. Endocrinol., 25, 221-228.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cDNA of skin fibroblast cDNA library inserted into pCMV expression vector, transiently transfected into and expressed in HEK-293 cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information rapid activity decrease upon homogenization Homo sapiens
0.0006
-
progesterone NADPH, intact cells Homo sapiens
0.0066
-
progesterone NADPH, cell homogenate Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48150
-
estimated by 1.3 kb cDNA Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
progesterone + NADPH Homo sapiens
-
20alpha-hydroxyprogesterone + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mammalia
-
-
-

Reaction

Reaction Comment Organism Reaction ID
17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17alpha-hydroxyprogesterone + NAD(P)H + H+ aldoketo reductase superfamily Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland 20alpha-HSD mRNA very low expressed Homo sapiens
-
brain 20alpha-HSD mRNA highly expressed Homo sapiens
-
gland 20alpha-HSD mRNA highly expressed Mammalia
-
HaCaT cell
-
Homo sapiens
-
liver 20alpha-HSD mRNA highly expressed Homo sapiens
-
prostate gland 20alpha-HSD mRNA lower expressed Homo sapiens
-
skin fibroblast Homo sapiens
-
testis 20alpha-HSD mRNA lower expressed Homo sapiens
-
uterus 20alpha-HSD mRNA lower expressed Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information negligible activity with dehydroepiandrosterone, estrone estradiol, 4-androstenendione, testosterone and diydrotestosterone as substrates Homo sapiens ?
-
?
progesterone + NAD(P)H NADPH much more efficient, 4-fold lower Km Homo sapiens 20alpha-hydroxyprogesterone + NAD(P)+
-
?
progesterone + NADPH
-
Homo sapiens 20alpha-hydroxyprogesterone + NADP+
-
?

Cofactor

Cofactor Comment Organism Structure
NADH
-
Homo sapiens
NADPH
-
Homo sapiens