Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-norepinephrine | 3 mM: 30% inhibition | Rattus norvegicus | |
catecholamine | - |
Rattus norvegicus | |
dopamine | slight; slight inhibition | Rattus norvegicus | |
Indoleamine | - |
Rattus norvegicus | |
Melatonin | 3 mM, almost complete inhibition; strong inhibition | Rattus norvegicus | |
additional information | no inhibition by 1-epinephrine, phenylalanine, tyrosine, L-DOPA, L-tryptophan, L-5-hydroxy-tryptophan | Rattus norvegicus | |
N-acetyl-serotonin | complete inhibition | Rattus norvegicus | |
N-acetylserotonin | 3 mM, complete inhibition | Rattus norvegicus | |
serotonin | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-(S)-lactoyl-7,8-dihydropterin + NADPH | Rattus norvegicus | terminal step in the biosynthetic pathway for tetrahydrobiopterin | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
6-(S)-lactoyl-7,8-dihydropterin + NADPH | Rattus norvegicus Sprague-Dawley | terminal step in the biosynthetic pathway for tetrahydrobiopterin | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
additional information | Rattus norvegicus | the enzyme might be involved in regulation of biosynthesis of catecholamines, indoleamines, and tetrahydrobiopterins | ? | - |
? | |
additional information | Rattus norvegicus Sprague-Dawley | the enzyme might be involved in regulation of biosynthesis of catecholamines, indoleamines, and tetrahydrobiopterins | ? | - |
? | |
sepiapterin + NADPH + H+ | Rattus norvegicus | - |
7,8-dihydrobiopterin + NADP+ | - |
? | |
sepiapterin + NADPH + H+ | Rattus norvegicus Sprague-Dawley | - |
7,8-dihydrobiopterin + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Sprague-Dawley | - |
Rattus norvegicus | - |
male sprague-dawley rats | - |
Rattus norvegicus Sprague-Dawley | - |
Sprague-Dawley | - |
Purification (Comment) | Organism |
---|---|
brain, partially, from erythrocytes to homogeneity | Rattus norvegicus |
native enzyme partially from brain by ammonium sulfate fractionation and hydroxylapatite chromatography, and from erythrocytes to homogeneity by affinity chromatography and isoelectric focusing | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
brain | in all parts of the brain | Rattus norvegicus | - |
erythrocyte | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-(S)-lactoyl-7,8-dihydropterin + NADPH | - |
Rattus norvegicus | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
6-(S)-lactoyl-7,8-dihydropterin + NADPH | terminal step in the biosynthetic pathway for tetrahydrobiopterin | Rattus norvegicus | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
6-(S)-lactoyl-7,8-dihydropterin + NADPH | - |
Rattus norvegicus Sprague-Dawley | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
6-(S)-lactoyl-7,8-dihydropterin + NADPH | terminal step in the biosynthetic pathway for tetrahydrobiopterin | Rattus norvegicus Sprague-Dawley | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
additional information | the enzyme might be involved in regulation of biosynthesis of catecholamines, indoleamines, and tetrahydrobiopterins | Rattus norvegicus | ? | - |
? | |
additional information | the enzyme might be involved in regulation of biosynthesis of catecholamines, indoleamines, and tetrahydrobiopterins | Rattus norvegicus Sprague-Dawley | ? | - |
? | |
sepiapterin + NADPH + H+ | - |
Rattus norvegicus | 7,8-dihydrobiopterin + NADP+ | - |
? | |
sepiapterin + NADPH + H+ | - |
Rattus norvegicus Sprague-Dawley | 7,8-dihydrobiopterin + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SR | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00017 | - |
N-acetyl-serotonin | pH 6.4, 25°C, erythrocyte enzyme | Rattus norvegicus | |
0.0002 | - |
N-acetyl-serotonin | pH 6.4, 25°C, brain enzyme | Rattus norvegicus | |
0.025 | - |
Melatonin | pH 6.4, 25°C, erythrocyte enzyme | Rattus norvegicus | |
0.03 | - |
Melatonin | pH 6.4, 25°C, brain enzyme | Rattus norvegicus | |
2.3 | - |
serotonin | pH 6.4, 25°C, brain enzyme | Rattus norvegicus | |
2.5 | - |
serotonin | pH 6.4, 25°C, erythrocyte enzyme | Rattus norvegicus | |
3.1 | - |
1-norepinephrine | pH 6.4, 25°C, erythrocyte enzyme | Rattus norvegicus | |
3.4 | - |
1-norepinephrine | pH 6.4, 25°C, brain enzyme | Rattus norvegicus |