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Literature summary for 1.1.1.153 extracted from

  • Kim, Y.A.; Chung, H.J.; Kim, Y.J.; Choi, Y.K.; Hwang, Y.K.; Lee, S.W.; Park, Y.S.
    Characterization of recombinant Dictyostelium discoideum sepiapterin reductase expressed in E. coli (2000), Mol. Cells, 10, 405-410.
    View publication on PubMed
Search for more literature for 1.1.1.153

Cloned(Commentary)

Cloned (Comment) Organism
recombinant protein expressed in Escherichia coli Dictyostelium discoideum

Inhibitors

Inhibitors Comment Organism Structure
Melatonin
-
 Dictyostelium discoideum
N-acetylserotonin
-
 Dictyostelium discoideum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 sepiapterin
-
 Dictyostelium discoideum
0.052
-
 NADPH
-
 Dictyostelium discoideum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29969
-
 x * 29969, determined by cDNA sequence Dictyostelium discoideum
30000
-
 x * 30000, Western blot analysis of native enzyme from crude cell extract Dictyostelium discoideum
32372
-
 x * 32372, SDS-PAGE, includes His-tag residues Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-(S)-lactoyl-7,8-dihydropterin + NADPH Dictyostelium discoideum
-
 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum
-
-
-
Phycomyces sp.
-
-
-

Storage Stability

Storage Stability Organism
-70°C, after dialyzation against Pipes, pH 7.5, 10% glycerol Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-(S)-lactoyl-7,8-dihydropterin + NADPH
-
 Dictyostelium discoideum 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 32372, SDS-PAGE, includes His-tag residues Dictyostelium discoideum
? x * 30000, Western blot analysis of native enzyme from crude cell extract Dictyostelium discoideum
? x * 29969, determined by cDNA sequence Dictyostelium discoideum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Dictyostelium discoideum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 most active, sodium phosphate buffer Dictyostelium discoideum