Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetic acid | buffers that contain acetate inhibit activity | Drosophila melanogaster | |
Cd2+ | 1 mM inhibits entirely | Drosophila melanogaster | |
Fe3+ | 1 mM inhibits entirely | Drosophila melanogaster | |
additional information | Li+, Na+, Co2+, Mn2+, Ni2+ show slight inhibition at 1 mM | Drosophila melanogaster | |
NADPH | more than 0.05 mM causes substrate inhibition | Drosophila melanogaster | |
Sn2+ | 1 mM inhibits entirely | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
NADPH | - |
Drosophila melanogaster | |
0.075 | - |
sepiapterin | - |
Drosophila melanogaster |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38400 | - |
1 * 38400, SDS-PAGE | Drosophila melanogaster |
39000 | - |
gel filtration, nondissociating PAGE | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-(S)-lactoyl-7,8-dihydropterin + NADPH | Drosophila melanogaster | - |
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
6-(S)-lactoyl-7,8-dihydropterin + NADPH | Drosophila melanogaster Oregon R | - |
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Drosophila melanogaster Oregon R | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Drosophila melanogaster |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-erythro-7,8-dihydrobiopterin + NADP+ = sepiapterin + NADPH + H+ | enzyme also reduces carbonyl compounds of non-pteridine derivatives | Drosophila melanogaster |
Storage Stability | Organism |
---|---|
-40°C, in 50 mM potassium phosphate buffer, pH 6.9, at least stable for two months | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-cyclohexadione + NADPH | - |
Drosophila melanogaster | ? | - |
? | |
6-(S)-lactoyl-7,8-dihydropterin + NADPH | - |
Drosophila melanogaster | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
6-(S)-lactoyl-7,8-dihydropterin + NADPH | - |
Drosophila melanogaster Oregon R | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
diacetyl + NADPH | - |
Drosophila melanogaster | ? | - |
? | |
diacetyl + NADPH | - |
Drosophila melanogaster Oregon R | ? | - |
? | |
menadione + NADPH | - |
Drosophila melanogaster | ? | - |
? | |
menadione + NADPH | - |
Drosophila melanogaster Oregon R | ? | - |
? | |
methylglyoxal + NADPH | - |
Drosophila melanogaster | ? | - |
? | |
methylglyoxal + NADPH | - |
Drosophila melanogaster Oregon R | ? | - |
? | |
p-nitrobenzaldehyde + NADPH | - |
Drosophila melanogaster | p-nitrobenzyl alcohol + NADP+ | - |
? | |
phenylglyoxal + NADPH | - |
Drosophila melanogaster | ? | - |
? | |
phenylglyoxal + NADPH | - |
Drosophila melanogaster Oregon R | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38400, SDS-PAGE | Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Drosophila melanogaster |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | - |
half of activity remains within 30 min | Drosophila melanogaster |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.7 | 6.7 | optimium for activity, highest activity in phosphate buffer | Drosophila melanogaster |