Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)/pLysS | Chlorobaculum tepidum |
expressed in Escherichia coli BL21(DE3)/pLysS | Chlorobium limicola |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+ | Chlorobaculum tepidum | final step in the synthesis of tetrahydrobiopterin | L-threo-tetrahydrobiopterin + 2 NADP+ | no formation of the L-erythro isomer | ? | |
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+ | Chlorobium limicola | final step in the synthesis of tetrahydrobiopterin | L-threo-tetrahydrobiopterin + 2 NADP+ | no formation of the L-erythro isomer | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorobaculum tepidum | - |
- |
- |
Chlorobium limicola | Q5XPX7 | - |
- |
Chlorobium limicola | Q5XPX7 | complete ORF | - |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Chlorobaculum tepidum |
recombinant protein using His-tag | Chlorobium limicola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+ | - |
Chlorobaculum tepidum | L-threo-tetrahydrobiopterin + 2 NADP+ | no formation of the L-erythro isomer | ? | |
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+ | - |
Chlorobium limicola | L-threo-tetrahydrobiopterin + 2 NADP+ | no formation of the L-erythro isomer | ? | |
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+ | final step in the synthesis of tetrahydrobiopterin | Chlorobaculum tepidum | L-threo-tetrahydrobiopterin + 2 NADP+ | no formation of the L-erythro isomer | ? | |
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+ | final step in the synthesis of tetrahydrobiopterin | Chlorobium limicola | L-threo-tetrahydrobiopterin + 2 NADP+ | no formation of the L-erythro isomer | ? | |
sepiapterin + NADPH + H+ | - |
Chlorobaculum tepidum | 7,8-dihydrobiopterin + NADP+ | - |
? | |
sepiapterin + NADPH + H+ | - |
Chlorobium limicola | 7,8-dihydrobiopterin + NADP+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Chlorobaculum tepidum | |
NADPH | - |
Chlorobium limicola |