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Literature summary for 1.1.1.153 extracted from

  • Choi, Y.K.; Jun, S.R.; Cha, E.Y.; Park, J.S.; Park, Y.S.
    Sepiapterin reductases from Chlorobium tepidum and Chlorobium limicola catalyze the synthesis of L-threo-tetrahydrobiopterin from 6-pyruvoyltetrahydropterin (2005), FEMS Microbiol. Lett., 242, 95-99.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)/pLysS Chlorobaculum tepidum
expressed in Escherichia coli BL21(DE3)/pLysS Chlorobium limicola

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+ Chlorobaculum tepidum final step in the synthesis of tetrahydrobiopterin L-threo-tetrahydrobiopterin + 2 NADP+ no formation of the L-erythro isomer ?
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+ Chlorobium limicola final step in the synthesis of tetrahydrobiopterin L-threo-tetrahydrobiopterin + 2 NADP+ no formation of the L-erythro isomer ?

Organism

Organism UniProt Comment Textmining
Chlorobaculum tepidum
-
-
-
Chlorobium limicola Q5XPX7
-
-
Chlorobium limicola Q5XPX7 complete ORF
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Chlorobaculum tepidum
recombinant protein using His-tag Chlorobium limicola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+
-
Chlorobaculum tepidum L-threo-tetrahydrobiopterin + 2 NADP+ no formation of the L-erythro isomer ?
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+
-
Chlorobium limicola L-threo-tetrahydrobiopterin + 2 NADP+ no formation of the L-erythro isomer ?
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+ final step in the synthesis of tetrahydrobiopterin Chlorobaculum tepidum L-threo-tetrahydrobiopterin + 2 NADP+ no formation of the L-erythro isomer ?
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+ final step in the synthesis of tetrahydrobiopterin Chlorobium limicola L-threo-tetrahydrobiopterin + 2 NADP+ no formation of the L-erythro isomer ?
sepiapterin + NADPH + H+
-
Chlorobaculum tepidum 7,8-dihydrobiopterin + NADP+
-
?
sepiapterin + NADPH + H+
-
Chlorobium limicola 7,8-dihydrobiopterin + NADP+
-
?

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Chlorobaculum tepidum
NADPH
-
Chlorobium limicola