KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0083 | - |
dihydroxyacetone | at pH 6.5, in 50 mM potassium phosphate buffer, at 25°C | Gluconobacter oxydans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Gluconobacter oxydans | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
SDS-PAGE | Gluconobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
- |
- |
Gluconobacter oxydans IFO 3255 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography, hydroxyapatite column chromatography, BD Sepharose column chromatography, and DEAE-Sephadex A-50 gel filtration | Gluconobacter oxydans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.06 | - |
crude extract, at 25°C | Gluconobacter oxydans |
13.71 | - |
after 228.5fold purification, at 25°C | Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone + NADH + H+ | less than 10% of the enzyme activity of NADPH-DHAR with NADPH is observed with NADH | Gluconobacter oxydans | glycerol + NAD+ | - |
r | |
dihydroxyacetone + NADH + H+ | less than 10% of the enzyme activity of NADPH-DHAR with NADPH is observed with NADH | Gluconobacter oxydans IFO 3255 | glycerol + NAD+ | - |
r | |
dihydroxyacetone + NADPH + H+ | - |
Gluconobacter oxydans | glycerol + NADP+ | - |
r | |
dihydroxyacetone + NADPH + H+ | - |
Gluconobacter oxydans IFO 3255 | glycerol + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
dihydroxyacetone reductase | - |
Gluconobacter oxydans |
glycerol:NADP 2-oxidoreductase, dihydroxyacetone forming | - |
Gluconobacter oxydans |
NADP-dependent GLDH | - |
Gluconobacter oxydans |
NADPH-DHAR | - |
Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Gluconobacter oxydans |