Cloned (Comment) | Organism |
---|---|
gene 7alpha-HSD, located 11.9 kb upstream of the hsdA gene, DNA and amino acid sequence determination and analysis, phylogenetic analysis, subcloning in Escherichia coli strain HB101, sequence comparisons, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS | Comamonas testosteroni |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a 7alpha -HSD knockout mutant that shows decreased degradation of testosterone, estradiol and cholesterol compared to the wild-type | Comamonas testosteroni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
- |
Comamonas testosteroni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholate + NAD+ | Comamonas testosteroni | - |
3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+ | - |
r | |
cholate + NAD+ | Comamonas testosteroni ATCC 11996 | - |
3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | S5U393 | i.e. Pseudomonas testosteroni | - |
Comamonas testosteroni ATCC 11996 | S5U393 | i.e. Pseudomonas testosteroni | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS by metal affinity chromatography | Comamonas testosteroni |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | Comamonas testosteroni strain ATCC11996 is a Gram-negative bacterium which can use steroids as carbon and energy source | Comamonas testosteroni | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholate + NAD+ | - |
Comamonas testosteroni | 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+ | - |
r | |
cholate + NAD+ | - |
Comamonas testosteroni ATCC 11996 | 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 27000, recombinant His-tagged enzyme, SDS-PAGE | Comamonas testosteroni |
Synonyms | Comment | Organism |
---|---|---|
7alpha-HSD | - |
Comamonas testosteroni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme sequence contains an N-terminal Gly-X-X-X-Gly-X-Gly cofactor-binding motif | Comamonas testosteroni |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the short-chain dehydrogenase/reductase (SDR) superfamily | Comamonas testosteroni |
malfunction | a 7alpha -HSD knockout mutant shows decreased degradation of testosterone, estradiol and cholesterol compared to the wild-type | Comamonas testosteroni |
additional information | the enzyme sequence contains a Tyr-X-X-X-Lys segment, residues 161-165, that is essential for catalytic activity of SDR proteins | Comamonas testosteroni |