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Literature summary for 1.1.1.159 extracted from

  • Ji, W.; Chen, Y.; Zhang, H.; Zhang, X.; Li, Z.; Yu, Y.
    Cloning, expression and characterization of a putative 7alpha-hydroxysteroid dehydrogenase in Comamonas testosteroni (2014), Microbiol. Res., 169, 148-154.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene 7alpha-HSD, located 11.9 kb upstream of the hsdA gene, DNA and amino acid sequence determination and analysis, phylogenetic analysis, subcloning in Escherichia coli strain HB101, sequence comparisons, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS Comamonas testosteroni

Protein Variants

Protein Variants Comment Organism
additional information construction of a 7alpha -HSD knockout mutant that shows decreased degradation of testosterone, estradiol and cholesterol compared to the wild-type Comamonas testosteroni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
-
Comamonas testosteroni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholate + NAD+ Comamonas testosteroni
-
3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
-
r
cholate + NAD+ Comamonas testosteroni ATCC 11996
-
3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni S5U393 i.e. Pseudomonas testosteroni
-
Comamonas testosteroni ATCC 11996 S5U393 i.e. Pseudomonas testosteroni
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS by metal affinity chromatography Comamonas testosteroni

Source Tissue

Source Tissue Comment Organism Textmining
additional information Comamonas testosteroni strain ATCC11996 is a Gram-negative bacterium which can use steroids as carbon and energy source Comamonas testosteroni
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholate + NAD+
-
Comamonas testosteroni 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
-
r
cholate + NAD+
-
Comamonas testosteroni ATCC 11996 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
-
r

Subunits

Subunits Comment Organism
? x * 27000, recombinant His-tagged enzyme, SDS-PAGE Comamonas testosteroni

Synonyms

Synonyms Comment Organism
7alpha-HSD
-
Comamonas testosteroni

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme sequence contains an N-terminal Gly-X-X-X-Gly-X-Gly cofactor-binding motif Comamonas testosteroni

General Information

General Information Comment Organism
evolution the enzyme is a member of the short-chain dehydrogenase/reductase (SDR) superfamily Comamonas testosteroni
malfunction a 7alpha -HSD knockout mutant shows decreased degradation of testosterone, estradiol and cholesterol compared to the wild-type Comamonas testosteroni
additional information the enzyme sequence contains a Tyr-X-X-X-Lys segment, residues 161-165, that is essential for catalytic activity of SDR proteins Comamonas testosteroni