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Literature summary for 1.1.1.169 extracted from
- Substrate specificity and kinetic isotope effect analysis of the Eschericia coli ketopantoate reductase (2003), Biochemistry, 42, 11289-11296.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in strain BL21(DE3) | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetics, pH-dependence of kinetics with different substrates, overview | Escherichia coli | |
| 0.004 | - |
beta-NADPH | pH 7.5, 25°C, recombinant enzyme | Escherichia coli |  |
| 0.018 | - |
thio-NADPH | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
| 0.036 | - |
alpha-NADPH | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
| 0.075 | - |
3'-NADPH | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
| 0.12 | - |
2-oxopantoate | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
| 3.8 | - |
3-methyl-2-oxo-n-valerate | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
| 8.7 | - |
2-oxoisovalerate | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxopantoate + NADPH | Escherichia coli | part of the pantothenate biosynthesis | (R)-pantoate + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene panE | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoisovalerate + NADPH | low activity | Escherichia coli | 2-hydroxyvalerate + NADP+ | - |
r | |
| 2-oxopantoate + 3'-NADPH | - |
Escherichia coli | (R)-pantoate + 3'-NADP+ | - |
r | |
| 2-oxopantoate + alpha-NADPH | - |
Escherichia coli | (R)-pantoate + alpha-NADP+ | - |
r | |
| 2-oxopantoate + beta-NADPH | highly specific for | Escherichia coli | (R)-pantoate + beta-NADP+ | - |
r | |
| 2-oxopantoate + NADPH | part of the pantothenate biosynthesis | Escherichia coli | (R)-pantoate + NADP+ | - |
? | |
| 2-oxopantoate + thio-NADPH | - |
Escherichia coli | (R)-pantoate + thio-NADP+ | - |
r | |
| 3-methyl-2-oxo-n-valerate + NADPH | low activity | Escherichia coli | 2-hydroxy-3-methyl-n-valerate + NADP+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ketopantoate reductase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH-dependence of kinetics with different substrates, modeling, overview | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 3'-NADPH | - |
Escherichia coli | |
| alpha-NADPH | - |
Escherichia coli | |
| beta-NADPH | preference for beta-NADPH as cofactor | Escherichia coli | |
| thio-NADPH | - |
Escherichia coli | |
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