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Literature summary for 1.1.1.17 extracted from
- D-Mannitol 1-phosphate dehydrogenase and D-sorbitol 6-phosphate dehydrogenase in Aerobacter aerogenes (1962), J. Biol. Chem., 237, 1342-1350.
General Stability
| General Stability | Organism |
|---|---|
| loss of activity partially protected by addition of substrates | Klebsiella aerogenes |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
NADH | cosubstrate: D-fructose 6-phosphate | Klebsiella aerogenes |  |
| 0.38 | - |
NAD+ | cosubstrate: D-mannitol 1-phosphate | Klebsiella aerogenes | |
| 2 | - |
D-mannitol 1-phosphate | cosubstrate: NAD+ | Klebsiella aerogenes | |
| 2.1 | - |
D-fructose 6-phosphate | cosubstrate: NADH | Klebsiella aerogenes | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Klebsiella aerogenes | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
sedimentation equilibrium analysis | Klebsiella aerogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mannitol 1-phosphate + NAD+ | Klebsiella aerogenes | - |
D-fructose 6-phosphate + NADH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella aerogenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography techniques | Klebsiella aerogenes |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | inducible by D-mannitol | Klebsiella aerogenes | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
1 unit defined as change of 0.01 in optical density at 340 nm during 30-90 second interval | Klebsiella aerogenes |
| additional information | - |
240 U/mg protein | Klebsiella aerogenes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 0.05 M Tris-HCl, pH 7.6, 0.05 M NaCl, 5 mM 2-mercaptoethanol, stable for weeks | Klebsiella aerogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mannitol 1-phosphate + 3-acetylpyridine-NAD+ | 7% of the activity compared to NAD+ | Klebsiella aerogenes | D-fructose 6-phosphate + ? | - |
? | |
| D-mannitol 1-phosphate + NAD+ | - |
Klebsiella aerogenes | D-fructose 6-phosphate + NADH + H+ | - |
r | |
| D-mannitol 1-phosphate + nicotinamide 6-(2-hydoxyethylamino) purine dinucleotide | 75% of the activity compared to NAD+ | Klebsiella aerogenes | D-fructose 6-phosphate + ? | - |
? | |
| D-mannitol 1-phosphate + nicotinamide hypoxanthine dinucleotide | 84% of the activity compared to NAD+ | Klebsiella aerogenes | D-fructose 6-phosphate + ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
mannitol 1-phosphate oxidation, pH depends on buffer system used | Klebsiella aerogenes |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | strictly specific | Klebsiella aerogenes | |
| NADH | strictly specific | Klebsiella aerogenes | |
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Release 2023.1 (January 2023)
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