General Stability | Organism |
---|---|
loss of activity partially protected by addition of substrates | Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
NADH | cosubstrate: D-fructose 6-phosphate | Klebsiella aerogenes | |
0.38 | - |
NAD+ | cosubstrate: D-mannitol 1-phosphate | Klebsiella aerogenes | |
2 | - |
D-mannitol 1-phosphate | cosubstrate: NAD+ | Klebsiella aerogenes | |
2.1 | - |
D-fructose 6-phosphate | cosubstrate: NADH | Klebsiella aerogenes |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Klebsiella aerogenes | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
sedimentation equilibrium analysis | Klebsiella aerogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + NAD+ | Klebsiella aerogenes | - |
D-fructose 6-phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Klebsiella aerogenes |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | inducible by D-mannitol | Klebsiella aerogenes | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
1 unit defined as change of 0.01 in optical density at 340 nm during 30-90 second interval | Klebsiella aerogenes |
additional information | - |
240 U/mg protein | Klebsiella aerogenes |
Storage Stability | Organism |
---|---|
4°C, 0.05 M Tris-HCl, pH 7.6, 0.05 M NaCl, 5 mM 2-mercaptoethanol, stable for weeks | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + 3-acetylpyridine-NAD+ | 7% of the activity compared to NAD+ | Klebsiella aerogenes | D-fructose 6-phosphate + ? | - |
? | |
D-mannitol 1-phosphate + NAD+ | - |
Klebsiella aerogenes | D-fructose 6-phosphate + NADH + H+ | - |
r | |
D-mannitol 1-phosphate + nicotinamide 6-(2-hydoxyethylamino) purine dinucleotide | 75% of the activity compared to NAD+ | Klebsiella aerogenes | D-fructose 6-phosphate + ? | - |
? | |
D-mannitol 1-phosphate + nicotinamide hypoxanthine dinucleotide | 84% of the activity compared to NAD+ | Klebsiella aerogenes | D-fructose 6-phosphate + ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
mannitol 1-phosphate oxidation, pH depends on buffer system used | Klebsiella aerogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | strictly specific | Klebsiella aerogenes | |
NADH | strictly specific | Klebsiella aerogenes |