Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyldicarbonate | 60% inhibition at 1 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
NAD+ | cosubstrate: hexitol phosphate | Escherichia coli | |
0.8 | - |
hexitol phosphate | cosubstrate: NAD+ | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Escherichia coli | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + NAD+ | Escherichia coli | highly specific for both substrates | D-fructose 6-phosphate + NADH + H+ | - |
? | |
D-mannitol 1-phosphate + NAD+ | Escherichia coli LJ378 | highly specific for both substrates | D-fructose 6-phosphate + NADH + H+ | - |
? | |
hexitol phosphate + NAD+ | Escherichia coli | - |
? + NADH | - |
? | |
hexitol phosphate + NAD+ | Escherichia coli LJ378 | - |
? + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Escherichia coli | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
715 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + NAD+ | highly specific for both substrates | Escherichia coli | D-fructose 6-phosphate + NADH + H+ | - |
? | |
D-mannitol 1-phosphate + NAD+ | highly specific for both substrates | Escherichia coli LJ378 | D-fructose 6-phosphate + NADH + H+ | - |
? | |
hexitol phosphate + NAD+ | - |
Escherichia coli | ? + NADH | - |
? | |
hexitol phosphate + NAD+ | - |
Escherichia coli LJ378 | ? + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable during 20 min | Escherichia coli |
60 | - |
90% loss of activity after 20 min | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
D-mannitol 1-phosphate oxidation | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | strictly specific | Escherichia coli |