Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Escherichia coli | 5622 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + NAD+ | Escherichia coli | highly specific for both substrates | D-fructose 6-phosphate + NADH + H+ | - |
r | |
D-mannitol 1-phosphate + NAD+ | Escherichia coli | favours reverse reaction | D-fructose 6-phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
precipitation techniques | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Escherichia coli | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
1 unit defined as change of 0.01 in optical density at 340 nm during 30-90 second interval | Escherichia coli |
additional information | - |
860 U/mg protein | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + NAD+ | highly specific for both substrates | Escherichia coli | D-fructose 6-phosphate + NADH + H+ | - |
r | |
D-mannitol 1-phosphate + NAD+ | favours reverse reaction | Escherichia coli | D-fructose 6-phosphate + NADH + H+ | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
45% loss of activity in 10 min | Escherichia coli |
60 | - |
complete inactivation in 10 min | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | no sharp optimum | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | strictly specific | Escherichia coli | |
NADH | strictly specific | Escherichia coli |