Cloned (Comment) | Organism |
---|---|
gene mtld1, encoded in the mannitol operon, sequence comparison, real-time PCR expression analysis, expression as His6-tagged protein in Escherichia coli strain SG13009 | Vibrio cholerae serotype O1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | 5 and 10 mM concentrations of MgSO4, MnSO4, FeCl3, ZnCl2, CoCl2, and LiCl have no effect on the enzyme activity | Vibrio cholerae serotype O1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + NAD+ | Vibrio cholerae serotype O1 | - |
D-fructose 6-phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae serotype O1 | - |
gene mtlD | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain SG13009 by nickel affinity chromatography | Vibrio cholerae serotype O1 |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | gene mtlD is constitutively expressed even when no mannitol wis present in the growth medium | Vibrio cholerae serotype O1 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.32 | - |
recombinant enzyme, crude extract, pH 7.5, 30°C, fructose 6-phosphate reduction | Vibrio cholerae serotype O1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + NAD+ | - |
Vibrio cholerae serotype O1 | D-fructose 6-phosphate + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
mannitol-1-phosphate dehydrogenase | - |
Vibrio cholerae serotype O1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Vibrio cholerae serotype O1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at, reduction reaction | Vibrio cholerae serotype O1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the NAD+-binding consensus sequence GxGxxGxGxxG is localized within the highly conserved N-terminal portion of MtlD corresponding to the amino acid residues 9-19 | Vibrio cholerae serotype O1 | |
NADH | - |
Vibrio cholerae serotype O1 |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the medium chain dehydrogenases | Vibrio cholerae serotype O1 |