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Literature summary for 1.1.1.175 extracted from

  • Nygard, Y.; Maaheimo, H.; Mojzita, D.; Toivari, M.; Wiebe, M.; Resnekov, O.; Gustavo Pesce, C.; Ruohonen, L.; Penttilae, M.
    Single cell and in vivo analyses elucidate the effect of xylC lactonase during production of D-xylonate in Saccharomyces cerevisiae (2014), Metab. Eng., 25, 238-247.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
gene xylB, recombinnat expression in Saccharomyces cerevisiae strain VVT B-67002 Caulobacter vibrioides

Protein Variants

Protein Variants Comment Organism
additional information both D-xylono-gamma-lactone and D-xylonate are produced when the Caulobacter crescentus gene xylB encoding D-xylose dehydrogenase is expressed in Saccharomyces cerevisiae, with or without co-expressionof xylC (D-xylonolactonase). XylC facilitates rapid opening of the lactone and more D-xylonate is initially produced than in its absence. In vivo [1H]NMR analysis of cell extracts, culture media and intact cells is used for analysis. The lactone and linear forms of D-xylonic acid are produced, accumulated intracellularly, and partially exported within 15–60 min after D-xylose provision. Co-expression of xylB and xylC leads to rapid loss of pHluorin fluorescence and loss of vitality during production of D-xylonate. Loss of vitality in the presence of D-xylose is correlated to the extracellular pH, but fluorescence is lost from xylB and xylC expressing cells regardless of the extracellular condition. Method optimization, overview Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylose + NAD+ Caulobacter vibrioides
-
 D-xylonolactone + NADH + H+
-
 ?
D-xylose + NAD+ Caulobacter vibrioides NA1000
-
 D-xylonolactone + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Caulobacter vibrioides B8H1Z0
-
-
Caulobacter vibrioides NA1000 B8H1Z0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylose + NAD+
-
 Caulobacter vibrioides D-xylonolactone + NADH + H+
-
 ?
D-xylose + NAD+
-
 Caulobacter vibrioides NA1000 D-xylonolactone + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
D-xylose 1-dehydrogenase
-
 Caulobacter vibrioides
XylB
-
 Caulobacter vibrioides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 in vivo assay, cell growth at 30°C Caulobacter vibrioides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Caulobacter vibrioides

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Caulobacter vibrioides