Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is useful in production of ursodeoxycholic acid, a secondary bile acid, which is used as a drug for the treatment of various liver diseases | Clostridium sp. |
synthesis | the enzyme is useful in production of ursodeoxycholic acid, a secondary bile acid, which is used as a drug for the treatment of various liver diseases | Clostridium perfringens |
synthesis | the enzyme is useful in production of ursodeoxycholic acid, a secondary bile acid, which is used as a drug for the treatment of various liver diseases | Eggerthella lenta |
synthesis | the enzyme is useful in production of ursodeoxycholic acid, a secondary bile acid, which is used as a drug for the treatment of various liver diseases | [Clostridium] leptum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | development and evaluation of a selected multi-step reaction system for the synthesis of ursodeoxycholic acid, separation of each step by isolation of the intermediates using ultrafiltration membranes, overview | Clostridium perfringens |
additional information | development and evaluation of a selected multi-step reaction system for the synthesis of ursodeoxycholic acid, separation of each step by isolation of the intermediates using ultrafiltration membranes, overview | Eggerthella lenta |
additional information | development and evaluation of a selected multi-step reaction system for the synthesis of ursodeoxycholic acid, separation of each step by isolation of the intermediates using ultrafiltration membranes, overview | [Clostridium] leptum |
additional information | development and evaluation of a selected multi-step reaction system for the synthesis of ursodeoxycholic acid. Multistep conversion of cholic acid into ursodeoxycholic acid. Enzyme sources are 7alpha-HSDH from Clostridium absonum (crude extract), 12alpha-HSDH from Clostridium group P, 7beta-HSDH from Clostridium absonum, method, separation of each step by isolation of the intermediates using ultrafiltration membranes, overview | Clostridium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholate + NAD+ | Clostridium perfringens | - |
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+ | - |
? | |
cholate + NAD+ | Eggerthella lenta | - |
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+ | - |
? | |
cholate + NADP+ | Clostridium sp. | - |
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+ | - |
? | |
cholate + NADP+ | [Clostridium] leptum | - |
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+ | - |
? | |
cholate + NADP+ | Clostridium sp. ATCC 29733 | - |
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+ | - |
? | |
additional information | Clostridium sp. | multistep conversion of cholic acid into ursodeoxycholic acid. Enzyme sources are 7alpha-HSDH from Clostridium absonum (crude extract), 12alpha-HSDH from Clostridium group P, 7beta-HSDH from Clostridium absonum | ? | - |
? | |
additional information | Clostridium sp. ATCC 29733 | multistep conversion of cholic acid into ursodeoxycholic acid. Enzyme sources are 7alpha-HSDH from Clostridium absonum (crude extract), 12alpha-HSDH from Clostridium group P, 7beta-HSDH from Clostridium absonum | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | - |
- |
- |
Clostridium sp. | - |
group P C 48-50 | - |
Clostridium sp. ATCC 29733 | - |
group P C 48-50 | - |
Eggerthella lenta | - |
formerly Eubacterium lentum | - |
[Clostridium] leptum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholate + NAD+ | - |
Clostridium perfringens | 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+ | - |
? | |
cholate + NAD+ | - |
Eggerthella lenta | 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+ | - |
? | |
cholate + NADP+ | - |
Clostridium sp. | 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+ | - |
? | |
cholate + NADP+ | - |
[Clostridium] leptum | 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+ | - |
? | |
cholate + NADP+ | conversion of CA into 12-oxoursodeoxycholic acid | Clostridium sp. | 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+ | - |
? | |
cholate + NADP+ | conversion of CA into 12-oxoursodeoxycholic acid | Clostridium perfringens | 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+ | - |
? | |
cholate + NADP+ | conversion of CA into 12-oxoursodeoxycholic acid | Eggerthella lenta | 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+ | - |
? | |
cholate + NADP+ | conversion of CA into 12-oxoursodeoxycholic acid | [Clostridium] leptum | 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+ | - |
? | |
cholate + NADP+ | - |
Clostridium sp. ATCC 29733 | 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+ | - |
? | |
cholate + NADP+ | conversion of CA into 12-oxoursodeoxycholic acid | Clostridium sp. ATCC 29733 | 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+ | - |
? | |
additional information | multistep conversion of cholic acid into ursodeoxycholic acid. Enzyme sources are 7alpha-HSDH from Clostridium absonum (crude extract), 12alpha-HSDH from Clostridium group P, 7beta-HSDH from Clostridium absonum | Clostridium sp. | ? | - |
? | |
additional information | multistep conversion of cholic acid into ursodeoxycholic acid. Enzyme sources are 7alpha-HSDH from Clostridium absonum (crude extract), 12alpha-HSDH from Clostridium group P, 7beta-HSDH from Clostridium absonum | Clostridium sp. ATCC 29733 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
12alpha-HSDH | - |
Clostridium sp. |
12alpha-HSDH | - |
Clostridium perfringens |
12alpha-HSDH | - |
Eggerthella lenta |
12alpha-HSDH | - |
[Clostridium] leptum |
NAD+-dependent 12alpha-HSDH | - |
Clostridium perfringens |
NAD+-dependent 12alpha-HSDH | - |
Eggerthella lenta |
NADP+-dependent 12alpha-HSDH | - |
Clostridium sp. |
NADP+-dependent 12alpha-HSDH | - |
[Clostridium] leptum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NAD+-dependent 12alpha-HSDH | Clostridium perfringens | |
NAD+ | NAD+-dependent 12alpha-HSDH | Eggerthella lenta | |
NADP+ | NADP+-dependent 12alpha-HSDH | Clostridium sp. | |
NADP+ | NADP+-dependent 12alpha-HSDH | [Clostridium] leptum |